RESUMEN

We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia.

Asunto(s)

Ixodes/microbiología , Rickettsia/clasificación , Rickettsia/genética , Animales , Proteínas de la Membrana Bacteriana Externa/genética , California , Bases de Datos de Ácidos Nucleicos , Reacción en Cadena de la Polimerasa , ARN , ARN Ribosómico 16S , Rickettsia/aislamiento & purificación , Análisis de Secuencia