Asunto(s)
Articulación Atlantoaxoidea/lesiones , Inestabilidad de la Articulación/diagnóstico por imagen , Apófisis Odontoides/lesiones , Fracturas de la Columna Vertebral/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Adolescente , Articulación Atlantoaxoidea/diagnóstico por imagen , Articulación Atlantoaxoidea/cirugía , Femenino , Humanos , Inestabilidad de la Articulación/cirugía , Apófisis Odontoides/diagnóstico por imagen , Apófisis Odontoides/cirugía , Cuadriplejía/diagnóstico por imagen , Cuadriplejía/cirugía , Fracturas de la Columna Vertebral/cirugía , Fusión VertebralRESUMEN
BACKGROUND: Term and preterm neonates experience quantitative and qualitative neutrophil deficiencies resulting in part from decreased production of granulocyte colony-stimulating factor (G-CSF). In adults, G-CSF improves neutrophil function by up-regulating adhesion molecules. PATIENTS AND METHODS: To evaluate the effects of G-CSF on neonatal neutrophil adhesive phenotypes, cord blood samples were incubated with G-CSF or phosphate-buffered saline and stimulated with N-formyl-methionyl-leucyl-phenylalanine (FMLP), and adhesion molecules were evaluated by flow cytometry. RESULTS: In term and preterm neutrophils, G-CSF incubation increased beta2-integrin expression significantly compared with baseline and to a greater extent than observed in adult neutrophils. With FMLP stimulation, beta2-integrin expression increased even more in the G-CSF group. L-selectin expression decreased after G-CSF incubation and decreased even more with FMLP stimulation in the G-CSF group compared with the phosphate-buffered saline group in term and preterm samples, but not in adult samples. CONCLUSIONS: The data show that G-CSF increases expression of beta2-integrin and decreases expression of L-selectin on unstimulated and stimulated term and preterm neonatal neutrophils in vitro. Further study is required to determine whether G-CSF improves neonatal neutrophil function.