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2.
Biokhimiia ; 48(10): 1596-603, 1983 Oct.
Artículo en Ruso | MEDLINE | ID: mdl-6639986

RESUMEN

Thin-layer isoelectric focusing of chymotrypsin modified by stepwise acylation with acryloyl chloride or maleic anhydride revealed a high heterogeneity of modification products, with a maximal number of components near 50% of substituted amino groups. Disc electrophoresis failed to establish the products diversity and could not therefore be used for heterogeneity control. The activity of the modified enzyme towards proteins and low molecular weight substrates depended on the modification reagent and correlated with the electrostatic enzyme--substrate interaction. The low hydrolytic activity towards N-acetyl-L-tyrosine p-nitroanilide was due to the increase in the Michaelis constant; the value of the catalytic constant remained unchanged.


Asunto(s)
Quimotripsina/metabolismo , Acrilatos/farmacología , Acilación , Cinética , Anhídridos Maleicos/farmacología
6.
Probl Endokrinol (Mosk) ; 24(6): 19-22, 1978.
Artículo en Ruso | MEDLINE | ID: mdl-724671

RESUMEN

Kinin system was investigated in 82 patients suffering from diabetes mellitus of various severity. To make pathogenetically substantiated pharmacological correction of the kinin system activity prodectin--kinin antagonist--was used in these patients. A conclusion was drawn that the blood plasma kinin system was activated in the patients with moderately severe and severe diabetes. The activity of total kallikrein and kininogen became normal against the background of prodectin treatment. The blood plasma kinin system activity was normalized simultaneously with the improvement of the diabetic angiopathies course.


Asunto(s)
Carbamatos/uso terapéutico , Diabetes Mellitus/tratamiento farmacológico , Hipoglucemiantes , Cininas/sangre , Piridinolcarbamato/uso terapéutico , Adolescente , Adulto , Anciano , Diabetes Mellitus/sangre , Angiopatías Diabéticas/tratamiento farmacológico , Humanos , Calicreínas/sangre , Quininógenos/sangre , Lisina Carboxipeptidasa/sangre , Persona de Mediana Edad
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