RESUMEN

Promotion is any factor which results in the increased cellular replication of initiated or transformed cells. We argue that cytotoxicity is not a necessary component of promotion and that, therefore, the existence of a threshold for promotion is unlikely.

Asunto(s)

Neoplasias/inducido químicamente , Animales , Supervivencia Celular/efectos de los fármacos , Humanos , Mitosis , Mutagénesis

RESUMEN

Consideration must be exercised in determination of buffers and solutions used when carrying out enzyme-linked immunosorbent assays (ELISAs). A commercial monoclonal antibody kit for abscisic acid (Idetek, Inc.) gives significant false-positives with tricarboxylic acid cycle intermediates. The organic acids or contaminants interfered with ELISA assays for ABA as indicated by deviations in the slopes of standard curves of ABA in the organic acids. The interference, in the case of alpha-ketoglutarate, was caused by a contaminant. Of the organic buffers tested-Tris, Tricine, and Hepes-only Hepes showed false-positive ABA. In addition, we present data indicating the presence of ABA in commercial mannitol and provide a simple procedure for removal of the ABA.