RESUMEN
The use of various types of stimulation of fish spermatozoa in order to increase the survival of defrosted cells is a promising direction in the field of low-temperature preservation. In this work, the use of acoustic-mechanical effects on the sperm of sturgeons (in particular, sterlet) was studied for the first time. The technical characteristics of the assembled experimental device for acoustic-mechanical effects on fish sperm are given. As a result of this work, the optimal parameters of exposure to increase the preservation of defrosted germ cells were selected.
Asunto(s)
Criopreservación , Semen , Acústica , Animales , Peces , Masculino , Motilidad Espermática/fisiología , Espermatozoides/fisiologíaRESUMEN
The antioxidant and cryoprotective efficiencies of a 3,5-di-tert-butyl-4-hydroxyphenyl)methylenediphosphonic acid (MDPA) differ significantly for sperm cells of various species of sturgeon fish (Russian sturgeon, beluga and Stellate sturgeon). The ability of phosphorus-containing phenol MDPA to decrease the level of lipid peroxidation of sperm, beneficial effect on the activity indicators of the sperm of native sturgeon and of the defrosted one after deep freezing as well as on the fertility of sperm cells was shown.
Asunto(s)
Criopreservación , Preservación de Semen , Animales , Criopreservación/métodos , Crioprotectores/farmacología , Humanos , Masculino , Fenol , Fenoles/farmacología , Fósforo , Federación de Rusia , Motilidad Espermática , EspermatozoidesRESUMEN
A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein's medium. It was shown that the level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in beluga sperm was inversely proportional to the motility time of sperm cells. The fertility of beluga sperm increased 2 times upon the addition of phosphorus-containing phenol to a modified Stein's medium. The prospects of the new antioxidant application to improve cryoresistance of beluga sperm in the conditions of cryopreservation for its efficient protection from the peroxidation processes are discussed.