RESUMEN
Fatal toxoplasmosis was diagnosed in a captive three-toed sloth (Bradypus tridactylus), an arboreal species of the tropical rainforests of South and Central America. In view of its specialized lifestyle and ecosystem and its low metabolic rate, B. tridactylus would seem to warrant further investigation in respect of its susceptibility to Toxoplasma gondii.
Asunto(s)
Perezosos/parasitología , Toxoplasma/aislamiento & purificación , Toxoplasmosis Animal/patología , Enfermedad Aguda , Animales , Antígenos de Protozoos/análisis , Resultado Fatal , Técnicas para Inmunoenzimas/veterinaria , Hígado/parasitología , Hígado/patología , Pulmón/parasitología , Pulmón/patología , Bazo/parasitología , Bazo/patología , Toxoplasma/inmunología , Toxoplasma/fisiología , Toxoplasmosis Animal/inmunologíaRESUMEN
A peroxidase-antiperoxidase (PAP) technique was used to diagnose bacillary haemoglobinuria in formalin-fixed, paraffin-embedded liver tissues of cattle. The PAP method revealed Clostridium haemolyticum in the zone of liver necrosis characteristic of the disease and also in culture smears of this microorganism, but C. novyi type B, C. chauvoei, C. septicum and C. perfringens types B and C remained unstained by the PAP reaction. The PAP technique performed provides a specific, simple and rapid method to diagnose bacillary haemoglobinuria.
Asunto(s)
Enfermedades de los Bovinos/diagnóstico , Infecciones por Clostridium/veterinaria , Clostridium/aislamiento & purificación , Hemoglobinuria/veterinaria , Técnicas para Inmunoenzimas/veterinaria , Animales , Bovinos , Infecciones por Clostridium/diagnóstico , Hemoglobinuria/diagnóstico , Hígado/microbiologíaRESUMEN
Various methods have been employed for the diagnosis of pseudorabies in Argentina. A large serological survey was carried out by means of enzyme-linked immunosorbent assay (blocking ELISA) and virus neutralisation (VN). An outbreak was studied by virological and immunohistochemical methods and in situ nucleic acid hybridisation.