RESUMEN
OBJECTIVE: To explore the influence of charred Gossamer urocteae (CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing. METHODS: CGU was extracted with different solvents and ethanol extract (EE), ethyl acetate fraction (EF), n-butanol fraction (BF) and aqueous fraction (AF) were obtained. The effects of different fractions on the proliferation, matrix metalloproteinase-2,9 (MMP-2,9) activities, synthesis of collagen and tissue inhibitor of metalloproteinase 1 (TIMP-1) in the mouse oral fibroblasts were determined by MTT, gelatin zymography, chloramine-T method, and enzyme-linked immunosorbent assay (ELISA) respectively. RESULTS: EE, EF and BF at high concentrations could significantly inhibit proliferation of fibroblasts (P<0.05 or P<0.01), and at low concentrations EF and BF could promote proliferation of fibroblasts, and BF and AF could significantly inhibit collagen synthesis (P<0.05 or P<0.01). EE, EF and AF at high concentrations could significantly increase the MMP-9 activity, and BF and AF could significantly inhibit synthesis of TIMP-1. CONCLUSION: CGU at high concentrations can inhibit the proliferations of fibroblasts and synthesis of collagen, and in healing of wound, CGU at high concentrations possibly has the functions of anti-fibrosis and anti-scar, and the mechanism to promote degradation of collagen is possibly related to the increase in MMP-9 activity and the inhibition of TIMP-1 synthesis.
Asunto(s)
Factores Biológicos/farmacología , Fibroblastos/efectos de los fármacos , Medicina Tradicional China , Boca/citología , Arañas/química , Animales , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Colágeno/metabolismo , Fibroblastos/enzimología , Fibroblastos/metabolismo , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Ratones , Ratones Endogámicos BALB C , Boca/efectos de los fármacos , Boca/enzimología , Boca/metabolismo , Inhibidor Tisular de Metaloproteinasa-1/metabolismoRESUMEN
BACKGROUND: Borneol, a widely used food and cosmetics additive, has analgesic, anti-inflammatory, antibacterial and penetration enhancing effects. We try to use it as a penetration enhancer for a formula which we have used to treat oral submucous fibrosis (OSF). To assess its safety, we investigate its effects on primary mice oral fibroblasts. METHODS: Primary mice oral fibroblasts were cultured, the effects of borneol on fibroblasts proliferation, cytotoxicity, collagen production, matrix metalloproteinases-2,9 (MMP-2,9) activities and tissue inhibitors of metalloproteinase 1 (TIMP-1) production were tested by methyl thiazolyl tetrazolium assay, lactic dehydrogenase activity assay, chloramine T method, gelatin zymography and enzyme-linked immunosorbent assay, respectively. RESULTS: Borneol, from 18.75 to 300 microg/ml, could significantly (P < 0.05) decrease the growth of fibroblasts and very significantly (P < 0.01) inhibit collagen production in a concentration dependant manner. From 18.75 to 150 microg/ml, borneol had no cytotoxicity on mice oral fibroblasts. Moreover borneol could significantly (P < 0.05) decrease MMP-2 activity, and very significantly (P < 0.01) inhibit TIMP-1 production. CONCLUSIONS: This study indicates that borneol has anti-fibrosis activity and the mechanism may partly be relevant to its inhibiting effects on fibroblasts mitosis, collagen and TIMP-1 production. It can be safely used as a penetration enhancer for our formula to treat OSF.
Asunto(s)
Canfanos/toxicidad , Portadores de Fármacos/toxicidad , Mucosa Bucal/efectos de los fármacos , Fibrosis de la Submucosa Bucal/tratamiento farmacológico , Perfumes/toxicidad , Animales , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Colágeno/antagonistas & inhibidores , Fibroblastos/efectos de los fármacos , Fibroblastos/enzimología , Inhibidores de la Metaloproteinasa de la Matriz , Ratones , Ratones Endogámicos BALB C , Mucosa Bucal/citología , Mucosa Bucal/enzimología , Permeabilidad/efectos de los fármacos , Inhibidor Tisular de Metaloproteinasa-1/antagonistas & inhibidoresRESUMEN
Endophyte JJ18 from Pseudolarix kaempferi was inoculated on solid medium (A), sterile soil (B), and non-sterile soil (C), respectively. The molluscicidal efficacy of fermented products of soil extract was detected according to the immersion test method recommended by WHO, the survival time of JJ18 was observed. Control groups included non-sterile soil (D), ionic water without chloride ion (E) and niclosamide (F). The acute toxicity of A-D group against Artemia salina was determined with artificial seawater as control. After immersing for 24 h, 48 h and 72 h, the snail mortality of group B [(36.67+/-7.56)%, (63.33+/-4.65)%, and (90+/-0)%] was significantly higher than those of group D [(30+/-6.87)%, (33.33+/-5.68)%, and (56.67+/-8.55)%] (P<0.05, P<0.01). Endophyte JJ18 survived in soil for at least 30 days. The mortality of Artemia salina in group B [(25.24+/-3.74)%] was significantly lower than that of group A[(57.15+/-8.90)%] (P<0.01).