RESUMEN
INTRODUCTION: Nanoparticles are used in various fields such as chemistry, pharmacy, biotechnology, and food science since they provide higher sensitivity than traditional optical detection methods. Recently, synthesis of nanomaterials using green chemistry has become popular. Many phytochemical components are used in the synthesis of nanoparticles, including vitamins, proteins, polysaccharides, glycosides, essential oils and phenolic compounds. OBJECTIVE: A novel green nanotechnology-based method using quince seed mucilage (QSM) was designed for the determination of ascorbic acid in pharmaceutical preparations. QSM, a natural polysaccharide, was used as a bioreducing and stabilizing reagent in the proposed silver nanoparticle (SNP)-based method. METHOD: In the first stage of the developed method, silver(I) is reduced to silver(0) via QSM and spherical, homogeneous SNPs were prepared (QSM-SNPs). In the second stage of the developed method, SNPs nuclei were enlarged with the addition of ascorbic acid. The developed method was validated by performance parameters (linearity, recovery, and precision). Ascorbic acid determination was performed by measuring increase in absorbance at 420 nm. RESULTS: The limit of detection and limit of quantification for ascorbic acid were, respectively, found to be at 0.27 and 0.90 µM. The QSM-SNP-based method was successfully applied to effervescent tablets containing ascorbic acid. The standards of the excipients frequently used in pharmaceutical preparations did not interfere with the developed method. CONCLUSION: The developed QSM-SNP-based method satisfies the requirements of green nanotechnology. The developed QSM-SNP-based method is simple, fast, eco-friendly and low-cost.
Asunto(s)
Nanopartículas del Metal , Rosaceae , Ácido Ascórbico/análisis , Plata/análisis , Plata/química , Nanopartículas del Metal/química , Rosaceae/química , Semillas/química , Polisacáridos/análisis , Preparaciones Farmacéuticas/análisisRESUMEN
In recent years, the determination of 2,4,6-trinitrotoluene (TNT) explosive residues in various matrices has attracted great interest from the perspective of national security and public health. Here, a fluorescent polyethylenimine capped carbon quantum dots (PEI-C-dots) probe was synthesized by a microwave-assisted technique using polyethylenimine and citric acid precursors and used to detect TNT. The sensing mechanism of TNT is based on fluorescence quenching as a result of the donor-acceptor interaction between Meisenheimer anion form of TNT and PEI on the PEI-C-dots surface. The fluorescence quantum yield of the synthesized PEI-C-dots was 54% and the detection limit for TNT was 93 µg/L. It was observed that neither the nitramine group (HMX and RDX) explosives with similar structures nor common soil ions and camouflage agents interfered with the determination of TNT. The interference effect of picric acid was eliminated by removing it with a basic anion exchanger before the determination. This nanosensor allows rapid, simple, selective, and sensitive determination of TNT residues in complex matrices and has the potential to be converted into a kit format.