RESUMEN
Foraging provides a multitude of individual, social, and environmental benefits. With green spaces decreasing in the United Kingdom, there is an opportunity for roadside verges to become valuable foraging resources; however, there is public concern over the safety of roadside forage. Human ingestion of heavy metal contaminants, such as copper (Cu), lead (Pb), and cadmium (Cd), originating from traffic activity, induces toxic effects in the body. Therefore, maximum Cu, Pb, and Cd guideline limits for human consumption in small fruits were established. However, studies of heavy metal concentrations in roadside forage and the effects of surface washing on concentrations are limited. This study examined Cu, Pb, and Cd in washed and unwashed wild blackberries (Rubus fruticose L.) along a main road in Kent, UK, and compares the values with maximum guideline limits. In all 44 samples, Cu, Pb and Cd concentrations were well below the maximum guideline limit or daily reference intake (RI) value. Cu and Pb concentrations were below the maximum daily intake in every sample if foragers eat one portion (80 g) of berries a day, but consuming a larger quantity of berries per day (e.g., 1 kg) could lead to an intake above the guideline limit for Cu (1 mg) and Pb (0.1 mg), but not Cd (0.03 mg). Washing did not significantly reduce the concentrations of Cu (p = 0.174) or Cd (p = 0.752) in blackberries, but did significantly reduce the Pb concentration (p < 0.001). However, Pb concentration was below maximum guideline limits for every sample regardless of washing treatment. Thus, wild blackberries collected from the roadside were suitable for human consumption, although the findings are not representative of all foraged berries or road networks. Integr Environ Assess Manag 2024;00:1-9. © 2024 The Author(s). Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
RESUMEN
This study reports the first complete genome sequence of nerine yellow stripe virus (NeYSV, GenBank MT396083). The genome consists of 10,165 nucleotides, excluding the 3'-terminal poly(A) tail. A single open reading frame encodes a large polyprotein of 3294 amino acids with typical potyvirus features. The nuclear inclusion b and coat protein region shares 95% identity with a previously reported partial NeYSV sequence (NC_043153.1). Phylogenetic analysis of the polyprotein amino acid sequence showed that NeYSV clustered with hippeastrum mosaic virus (HiMV YP_006382256.1).