RESUMEN
Nuts are highly valued for their properties and are consumed worldwide owing to their health benefits, particularly, for the prevention and treatment of diseases, as well as a source of essential macro- and micronutrients. This study aimed to evaluate the effects of roasting on the nutritional value of nuts. Three types of nuts, raw and roasted almonds, cashews, and walnuts were selected from three well-known companies in Saudi Arabia. To analyze and evaluate the stability of macro- and micronutrients in nuts, modern separation and quantification methods, including inductively coupled plasma-optical emission spectrometry (ICP-OSE), were used to analyze and evaluate the stability of nutrients and heavy metals in nuts that are widely distributed and consumed. The results of this study indicate that nuts have high nutritional value and an inverse roasting effect. Comparing the nutrient and metal contents of raw and roasted nuts provides useful information on the nutritional science of nuts consumed by well-known manufacturers in the Kingdom of Saudi Arabia. Further investigations of nuts are needed to draw firm conclusions regarding their association with the prevention of many different diseases.
RESUMEN
Introduction: Foodborne trichothecene T-2 Toxin, is a highly toxic metabolite produced by Fusarium species contaminating animal and human food, causing multiple organ failure and health hazards. T-2 toxins induce hepatotoxicity via oxidative stress causing hepatocytes cytotoxicity and genotoxicity. In this study, curcumin and taurine were investigated and compared as antioxidants against T-2-provoked hepatotoxicity. Methods: Wistar rats were administrated T-2 toxin sublethal oral dose (0.1 mg/kg) for 2 months, followed by curcumin (80 mg/kg) and taurine (50 mg/kg) for 3 weeks. Biochemical assessment of liver enzymes, lipid profiles, thiobarbituric acid reactive substances (TBARs), AFU, TNF-α, total glutathione, molecular docking, histological and immunohistochemical markers for anti-transforming growth factor-ß1 (TGFß1), double-strand DNA damage (H2AX), regeneration (KI67) and apoptosis (Active caspase3) were done. Results and Discussion: Compared to T-2 toxin, curcumin and taurine treatment significantly ameliorated hepatoxicity as; hemoglobin, hematocrit and glutathione, hepatic glycogen, and KI-67 immune-reactive hepatocytes were significantly increased. Although, liver enzymes, inflammation, fibrosis, TGFß1 immunoexpressing and H2AX and active caspase 3 positive hepatocytes were significantly decreased. Noteworthy, curcumin's therapeutic effect was superior to taurine by histomorphometry parameters. Furthermore, molecular docking of the structural influence of curcumin and taurine on the DNA sequence showed curcumin's higher binding affinity than taurine. Conclusion: Both curcumin and taurine ameliorated T-2 induced hepatotoxicity as strong antioxidative agents with more effectiveness for curcumin.