RESUMEN
Presumptive evidence for functional germ cell chimerism in the marmoset was evaluted by calculating the offspring sex ratio of young derived from animals in which the blood chimerism status was known. In the four possible mating combinations involving chimeric and non-chimeric marmosets, there was no apparent deviation from the anticipated 1:1 sex ratio. Analysis of 2,500 primary spermatocytes from ten known blood chimeras failed to reveal a single cell in which an unequivocal XX bivalent could be identified. The combined breeding and cytogenetic data offer presumptive evidence against functional germ cell chimerism in the marmoset.
Asunto(s)
Callitrichinae/genética , Quimera , Espermatocitos/citología , Espermatozoides/citología , Animales , Callitrichinae/sangre , Femenino , Haplorrinos , Linfocitos/citología , Masculino , Razón de Masculinidad , Cromosoma X , Cromosoma YRESUMEN
A regimen of thiacetarsamide sodium (0.22 ml/kg twice daily for 2 days) plus levamisole phosphate (11 mg/kg/day for 10 days) was effective in eliminating unidentified microfilariae from the blood in seven of eight tamarins. No serious side effects resulted from the treatment. All of the animals were initially freed of circulating microfilariae after treatment, and five have remained microfilaria-negative for 1 year. Two of the tamarins died of causes unrelated to filariasis and were microfilaria-negative before death. One tamarin remained microfilaria-positive after two courses of this treatment.
Asunto(s)
Arsenamida/uso terapéutico , Arsenicales/uso terapéutico , Callitrichinae , Filariasis/veterinaria , Levamisol/uso terapéutico , Enfermedades de los Monos/tratamiento farmacológico , Animales , Filariasis/tratamiento farmacológico , HaplorrinosAsunto(s)
Cruzamiento , Callitrichinae , Reproducción , Animales , Animales Recién Nacidos/fisiología , Callitrichinae/fisiología , Femenino , Haplorrinos , Humanos , Trabajo de Parto , Masculino , Edad Materna , Mortalidad , Embarazo , Preñez , Estaciones del Año , Razón de Masculinidad , GemelosRESUMEN
Marmosets have a high frequency of fraternal twinning, and placental vascular anastomoses between the twin fetuses invariably lead to hemopoietic chimerism. The occasional finding of chimerism in single-born marmosets suggested that in a twin pregnancy one fetus had undergone resorption after contributing hemopoietic stem cells to its twin. In this study non-chimeric single-born marmosets were produced by fallopian tube ligation or surgical relocation of one ovary in breeding females. Further, in an examination of hemopoietic cells from over 50 single-born young from nonoperated females, chimerism occurred less frequently than what one would expect if resorption of a co-twin had occurred after a functional anastomosis had been established.