RESUMEN
Ten biochemical parameters total protein, albumin, glucose, cholesterol, urea, creatinine, total bilirubin, ALT, AST, APh were determined during long-term light mode changes in serum of rats. Changing the light mode, a number of parameters revealed unreliable 24-hour rhythms. An ultradian 12-hour reliable rhythm has been for serum total protein of rats exposed to constant darkness during 1 month. The light-modified model can be used to study the effects of the chemical factor in combination with the physical factor of the external environment, as well as in preclinical toxicity tests of medicinal substances in different light modes.
Asunto(s)
Fenómenos Biomecánicos , Animales , Colesterol , Creatinina , Glucosa , RatasRESUMEN
This article describes models for the study of acute desynchronosis: jetlag syndrome and acute desynchronosis under physical stress for possible pharmacological correction of these disorders. The cosinor analysis allowed assessing significance of changes in biological rhythms in 2 biological models: the jetlag-type diurnal rhythm shift model and the model with changed light mode. The revealed changes in the rhythms of biochemical parameters in the blood serum of animals with acute desynchronosis indicate significant changes in the intensity of carbohydrate-lipid metabolism, which affected the processes of cell bioenergetics. These changes are most pronounced in the group of animals that were kept under conditions of constant darkness, which can serve as a marker of the initial stage of pathological desynchronosis. The jetlag-type model can be used to evaluate the effectiveness of the pharmacological correction of physiological desynchronosis. The model with modified light regimen can be used for evaluation of the effectiveness of pharmacological correction of pathological desynchronosis.
Asunto(s)
Ritmo Circadiano/fisiología , Oscuridad , Síndrome Jet Lag/sangre , Síndrome Jet Lag/fisiopatología , Alanina Transaminasa/metabolismo , Albúminas/metabolismo , Animales , Metabolismo de los Hidratos de Carbono , Creatinina/metabolismo , Hipotálamo/metabolismo , Luz , Metabolismo de los Lípidos , Masculino , Periodicidad , Ratas , Ratas Wistar , Programas Informáticos , Núcleo Supraquiasmático/metabolismo , Factores de TiempoRESUMEN
Expression of genes encoding the individual subunits of ionotropic GABAA receptor was assessed after acute and chronic intoxication of rats with ethanol. The chronic 1-month-long exposure to ethanol signifi cantly decreased (by 38%) expression of Gabrb1 gene in the hippocampus. Acute exposure to ethanol elevated expression of genes Gabrb1 (by 1.7 times), Gabra1 (by 3.8 times), and Gabra4 (by 6.5 times), although it diminished expression of Gabra2 gene by 1.4 times. In preliminarily alcoholized rats, acute intoxication with ethanol enhanced expression of genes Gabrb1 and Gabra5 by 1.7 and 8.7 times, respectively. There was neither acute nor chronic effect of ethanol on expression of gene Gabra3.
Asunto(s)
Etanol/efectos adversos , Regulación de la Expresión Génica/efectos de los fármacos , Expresión Génica/efectos de los fármacos , Receptores de GABA-A/biosíntesis , Intoxicación Alcohólica/metabolismo , Animales , Hipocampo/metabolismo , Masculino , Ratas , Receptores de GABA-A/genéticaRESUMEN
We performed immunohistochemical analysis of the expression of caspases 3, 9 and bcl-2 protein in rat brain at various terms after administration of LD50 of sodium thiopental. Expression of the specified apoptosis markers was found in the sensorimotor cortex and hippocampus (dentate gyrus and CA2 region).