RESUMEN
We studied immunocorrecting effects of Semax (Met-Glu-His-Phe-Pro-Gly-Pro) on the model of "social" stress caused by sensory contact and intermale confrontation. Functional activity of the immune system of laboratory animals was evaluated in standard immunopharmacological tests: delayed-type hypersensitivity reaction, direct agglutination test, latex test for studying phagocytic activity of peripheral blood neutrophils, changes in differential leukocyte count, and weight of immunocompetent organs. It was found that changes in the immune response caused by "social" stress are multidirectional, which confirms the theory of stress-induced "immune imbalance". Semax acted as effective immune corrector restoring cellular and humoral immunogenesis reactions and phagocytic activity of neutrophils. This attested to the presence of immunomodulating properties in Semax and necessitates further studies in this field.
Asunto(s)
Hormona Adrenocorticotrópica/análogos & derivados , Factores Inmunológicos/farmacología , Fármacos Neuroprotectores/farmacología , Nootrópicos/farmacología , Fragmentos de Péptidos/farmacología , Estrés Psicológico/tratamiento farmacológico , Hormona Adrenocorticotrópica/farmacología , Agresión , Animales , Animales no Consanguíneos , Hipersensibilidad Tardía/tratamiento farmacológico , Hipersensibilidad Tardía/inmunología , Inmunidad Innata/efectos de los fármacos , Pruebas de Fijación de Látex , Recuento de Leucocitos , Masculino , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Fagocitosis/efectos de los fármacos , Estrés Psicológico/inmunología , Estrés Psicológico/fisiopatologíaRESUMEN
The study was aimed to investigate the influence of drug reamberin inclusion in the treatment regimen of patients with acute Epstein-Barr virus (EBV) infection on the effectiveness of therapy. Treatment results were analyzed in a group of 70 children aged 4-15 with a diagnosis of moderate to severe EBV infection. By the method of random sampling distribution, patients were divided into two comparable groups of 35 children, which were representative with respect to gender, age, date of admission, and conducted basic therapy. Patients in the control group were treated by the conventional scheme, while the main group received basic therapy with antibacterial drug (according to indication) and symptomatic agents (antipyretics, desensitizing agents, and local antiseptics for the treatment of rotor and nasopharynx) and, in addition, obtained 1.5% reamberin solution intravenously, 10 mL/kg body weight once a day at a rate of 3-4 mL/min (the treatment course did not exceed 3 days). Treatment efficacy was assessed by a decrease in the duration of intoxication symptoms, relief of their clinical manifestations, and normalization of laboratory data (including, in addition to commonly accepted data, the levels of malonic dialdehyde, ferritin, transferrin and catalase before and after treatment).The inclusion of reamberin in the therapy of acute EBV infection in children favors (in comparison to conventional treatment regimen) more pronounced and rapid decrease the intensity of the oxidative process and improves the functioning of the antioxidant system. This was manifested by normalization of immunobiochemical indicators (reduction of malonic dialdehyde and ferritin and increase in the level of catalase) and decrease in the inflammatory response (leukocytosis, ESR, and the number of atypical mononuclear cells in the blood), This resulted in more rapid relief of the clinical manifestations of infection (sore throat, hyperthermia, lymphadenopathy, and hepatomegaly) and shortened the hospital stay by 38.5% (p < 0.05).
Asunto(s)
Infecciones por Virus de Epstein-Barr/tratamiento farmacológico , Herpesvirus Humano 4 , Meglumina/análogos & derivados , Enfermedades Metabólicas/tratamiento farmacológico , Succinatos/administración & dosificación , Enfermedad Aguda , Adolescente , Niño , Preescolar , Infecciones por Virus de Epstein-Barr/complicaciones , Femenino , Humanos , Masculino , Meglumina/administración & dosificación , Enfermedades Metabólicas/complicacionesRESUMEN
AIM: Development of enzyme immunoassay detection of B and D factors of complement alternative pathway functional activity for solving diagnostic and prognostic problems of patient therapy. Study activity of these factors in blood sera of children with atopic dermatitis before and after therapy for elucidation of the role of complement alternative pathway in pathogenesis of this disease. MATERIALS AND METHODS: Children aged 6 months to 18 years with atopic dermatitis were examined for functional activity of B and D factors in blood sera before and after therapy by the developed methods. RESULTS: The developed enzyme immunoassay methods for determination of functional activity of B and D complement alternative pathway showed high sensitivity and reliability. In children with atopic dermatitis factor B and D activity was significantly lower than normal before treatment. After treatment these activity increased significantly (p < 0.004) and in the case of D factor--up to normal. CONCLUSION: The data obtained in the study indicates the presence of complement alternative pathway activation in atopic dermatitis in children and the possibility of use of factor B and D functional activity analysis for diagnostic and prognostic purposes.
Asunto(s)
Factor B del Complemento/aislamiento & purificación , Factor D del Complemento/aislamiento & purificación , Vía Alternativa del Complemento , Dermatitis Atópica/sangre , Adolescente , Niño , Preescolar , Dermatitis Atópica/patología , Dermatitis Atópica/terapia , Humanos , LactanteRESUMEN
The efficacy of a 1.5% reamberin solution possessing detoxicating, antihypoxant, antioxidant, and membrane-protective properties, used as a component of the complex infusion therapy in children with acute Epstein-Barr virus infection, has been estimated. The dynamics of the inflammatory process in children treated by the drug complex including reamberin is characterized by rapid relief of general symptoms, improvement of state, and reduction in length of the febrile period of disease as compared to patients treated with glucose-saline solutions and basic therapy. The administration of reamberin in children contributed to the early elimination of metabolic shifts by the period of convalescence, as evidenced by improvement in some laboratory parameters, including protein-synthesis function of the liver.
Asunto(s)
Antivirales/administración & dosificación , Infecciones por Virus de Epstein-Barr/tratamiento farmacológico , Herpesvirus Humano 4 , Meglumina/análogos & derivados , Succinatos/administración & dosificación , Adolescente , Niño , Preescolar , Infecciones por Virus de Epstein-Barr/metabolismo , Infecciones por Virus de Epstein-Barr/patología , Femenino , Humanos , Lactante , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Inflamación/patología , Inflamación/virología , Hígado/metabolismo , Hígado/patología , Masculino , Meglumina/administración & dosificación , Biosíntesis de Proteínas/efectos de los fármacosRESUMEN
The results of the cycloferon use in therapy of 80 children at the age of 1 to 15 years with infectious mononucleosis are presented. The children were divided by chance sampling into two comparable groups of 40 subjects each. In the first group the children in addition to the standard therapy were treated with cycloferon for 10 days, intramuscularly or orally depending on the age. The reference group included the patients under the standard therapy alone. The efficacy of the drug was evaluated by reduction and more rapid disappearance of the main clinical symptoms of the disease. The improvement of certain laboratory indices, including those of the liver protein synthesis function were recorded, that allowed to reduce the hospitalization term. No side effects of the cycloferon use were observed.
Asunto(s)
Acridinas/uso terapéutico , Mononucleosis Infecciosa/tratamiento farmacológico , Inductores de Interferón/uso terapéutico , Acridinas/administración & dosificación , Administración Oral , Adolescente , Niño , Preescolar , Esquema de Medicación , Quimioterapia Combinada , Femenino , Humanos , Lactante , Inyecciones Intramusculares , Inductores de Interferón/administración & dosificación , Masculino , Resultado del TratamientoRESUMEN
The clinico-immunological effect and significance of the rectal and inhalation methods of the application of recombinant IFN-alpha2 with complex immunoglobulin preparation (CIP) in the treatment of relapsing bronchitis in children have been determined. The determination of the levels of interferon activity in buccal secretions and in venous blood permits the objective evaluation of the immune responsiveness of the body and the treatment effect. The immunocorrecting action of the combined immunobiological preparation (recombinant IFN-alpha2 and CIP) is manifested by an increased level of endogenic interferon and the stimulation of phagocytic activity.
Asunto(s)
Bronquitis Crónica/terapia , Inmunoglobulinas/uso terapéutico , Interferón Tipo I/uso terapéutico , Administración por Inhalación , Administración Rectal , Aerosoles/uso terapéutico , Bronquitis Crónica/inmunología , Bronquitis Crónica/prevención & control , Niño , Preescolar , Quimioterapia Combinada , Femenino , Humanos , Inmunoglobulinas/administración & dosificación , Interferón Tipo I/administración & dosificación , Interferones/análisis , Recuento de Leucocitos , Masculino , Fagocitos/inmunología , Proteínas Recombinantes , Saliva/inmunología , Prevención Secundaria , Supositorios/uso terapéuticoRESUMEN
Apart from lymphadenopathy types observed in well-known diseases (oncology and hematology pathologies, systemic lesions of connective tissues, tuberculosis, brucellosis, infection mononucleosis, toxoplasmosis, AIDS and other specific processes), there is an extensive group of lymphadenopathy (up to 70% of all disease cases), whose cause cannot be diagnosed by using the routine examination schemes. This was reflected in the diagnosis of "lymphadenopathy of unverified etiology" according to the MKB-1 now in force. Investigations of the role (in etiopathogenesis) of unverified lymphadenopathy types as observed in children with the infection component as well as optimization of their diagnosis and treatment schemes are elucidated in the paper. It is substantiated, on the basis of published data and authors' independent research, that some of lymphadenopathy types of unverified etiology are related with such opportunistic infections, like clamidiosis, mycoplasmosis, herpes simplex and cytomegalovirus infection. The above stated is, primarily, compatible with the tropicity of such infections' agents to the lymphoid tissue and/or with their possible lymphogenic spread as well as with polymorphism of clinical signs conditioned by the discussed infection agents.