RESUMEN
The anomalous origin of the pulmonary trunk in the ascending aorta, defined as arterious hemitruncus, is a rare congenital malformation in dogs, caused by a defect in the spiral septum. Thus, given the unusual occurrence in the canine species, the systemic severity and the high lethality, the aim of this study was to describe this heart disease in a three-month-old male German Spitz puppy, emphasizing clinical changes of the necropsy and microscopics. The animal had cyanosis, dyspnea and weakness and was forwarded for necropsy after sudden death. Numerous changes were detected in the post-mortem examination, including in the heart, as cardiomegaly and absence of the arterial ligament, which was replaced by the complete fusion between the ascending aorta and the pulmonary trunk, after leaving both the left and right ventricles, respectively and, among the microscopic findings, cardiomyocyte hypertrophy stood out. The association of these findings with the history indicated the diagnosis of arterious hemitruncus followed by cardiorespiratory failure, emphasizing the importance of out complementary cardiological exams in young symptomatic patients for the survival of those affected. Arterious hemitruncus, although rare, must be added in the differential diagnosis of other heart diseases that cause similar clinical signs.(AU)
A origem anômala do tronco pulmonar em aorta ascendente, definida como hemitruncus arterioso, é uma malformação congênita rara em cães, causada por defeito no septo espiral. Assim, diante da ocorrência incomum na espécie canina, da gravidade sistêmica e da alta letalidade, o objetivo deste trabalho foi descrever essa doença cardíaca em um filhote de três meses de idade, macho, Spitz Alemão, enfatizando as alterações clínicas, de necropsia e microscópicas. O animal apresentava cianose, dispneia e fraqueza e foi encaminhado para necropsia após morte súbita. Inúmeras alterações foram detectadas no exame post-mortem, inclusive no coração, como cardiomegalia e ausência do ligamento arterioso, o qual foi substituído pela fusão completa entre aorta ascendente e tronco pulmonar, após a saída de ambas dos ventrículos esquerdo e direito, respectivamente, e, dentre os achados microscópicos, destacou-se a hipertrofia de cardiomiócitos. A associação desses achados com o histórico indicou o diagnóstico de hemitruncus arterioso seguido de insuficiência cardiorrespiratória, ressaltando-se a importância de exames complementares cardiológicos em pacientes jovens sintomáticos na sobrevida dos acometidos. O hemitruncus arterioso, apesar de raro, deve ser acrescido no diagnóstico diferencial de outras cardiopatias que causam sinais clínicos similares.(AU)
Asunto(s)
Animales , Perros , Aorta/anomalías , Arteria Pulmonar/anomalías , Defectos de los Tabiques Cardíacos/patología , Defectos de los Tabiques Cardíacos/veterinaria , Anomalías Congénitas/veterinariaRESUMEN
1. Sausages were used to assess the effects of chicken meat replacement by spent laying hen meat on the quality parameters and sensorial properties. 2. Five formulations were developed: control (CON) containing 100% chicken meat and 4 levels of chicken meat replacement with spent laying hen meat: 25% (T25), 50% (T50), 75% (T75) and 100% (T100). 3. Appearance, texture, succulence, flavour and overall consumer acceptance were analysed. Parameters such as moisture, ash, fat and protein content, pH, cooking loss and colour parameters (lightness (L*), redness (a*), yellowness (b*)) were analysed, as well as the texture properties (hardness, elasticity, cohesiveness and chewiness). 4. Statistical analysis did not reveal significant (P > 0.05) differences in chemical composition and colour parameters among formulations. On the other hand, CON treatment showed the lowest (P < 0.05) cooking loss (20.45%) that increased as spent laying hen meat increased in the formulation (24.92% vs. 27.65% vs. 28.12% vs. 33.05%, for T25, T50, T75 and T100 batches, respectively). 5. Regarding textural parameters, T75 and T100 formulations presented higher (P < 0.05) hardness and chewiness compared to the other ones. 6. Concerning to sensorial characteristics, the T100 formulation presented the lowest average scores (P < 0.05) for all attributes studied. However, the other batches (T25, T50 and T75) did not show significant differences for appearance, texture, succulence, flavour and overall acceptability attributes compared with the CON formulation. 7. The results indicated that the substitution of up to 75% of chicken meat by spent laying hen meat did not decrease the sensory acceptance of the sausages by consumers, demonstrating that this level of substitution in sausages allows better use of spent laying hen meat.
Asunto(s)
Manipulación de Alimentos , Productos Avícolas/análisis , Gusto , Animales , Pollos , Color , Culinaria , Femenino , HumanosRESUMEN
Studies have shown that autologous hematopoietic SCT (HSCT) can be used as an intensive immunosuppressive therapy to treat refractory patients and to prevent the progression of multiple sclerosis (MS). This is a prospective multicentric Brazilian MS trial comparing two conditioning regimens: BEAM/horse ATG and CY/rabbit ATG. Most (80.4%) of the 41 subjects in the study had the secondary progressive MS subtype and the mean age was 42 years. The baseline EDSS score in 58.5% of the subjects was 6.5 and 78% had a score of 6.0 or higher, respectively. The complication rate during the intra-transplantation period was 56% for all patients: 71.4% of the patients in the BEAM/hATG group and 40% in the CY/rATG group (P=0.04). Three subjects (7.5%) died of cardiac toxicity, sepsis and alveolar hemorrhage, all of them in the BEAM/ATG group. EFS was 58.54% for all patients: 47% in the BEAM/hATG group and 70% in the CY/rATG group (P=0.288). In conclusion, the CY/rATG regimen seems to be associated with similar outcome results, but presented less toxicity when compared with the BEAM/hATG regimen. Long-term follow-up would be required to fully assess the differences in therapeutic effectiveness between the two regimens.
Asunto(s)
Trasplante de Células Madre Hematopoyéticas/métodos , Esclerosis Múltiple/terapia , Acondicionamiento Pretrasplante/métodos , Adulto , Animales , Suero Antilinfocítico/administración & dosificación , Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Carmustina/administración & dosificación , Citarabina/administración & dosificación , Etopósido/administración & dosificación , Femenino , Rechazo de Injerto/prevención & control , Movilización de Célula Madre Hematopoyética , Caballos , Humanos , Masculino , Melfalán/administración & dosificación , Persona de Mediana Edad , Calidad de Vida , ConejosRESUMEN
We have previously isolated a Lys49 phospholipase A(2) homolog (BaTX) from Bothrops alternatus snake venom using a combination of molecular exclusion chromatography and reverse phase HPLC and shown its ability to cause neuromuscular blockade. In this work, we describe a one-step procedure for the purification of this toxin and provide further details of its neuromuscular activity. The toxin was purified by reverse phase HPLC and its purity and molecular mass were confirmed by SDS-PAGE, MALDI-TOF mass spectrometry, amino acid analysis and N-terminal sequencing. BaTX (0.007-1.4 microM) produced time-dependent, irreversible neuromuscular blockade in isolated mouse phrenic nerve-diaphragm and chick biventer cervicis preparations (time to 50% blockade with 0.35 microM toxin: 58+/-4 and 24+/-1 min, respectively; n=3-8; mean+/-S.E.) without significantly affecting the response to direct muscle stimulation. In chick preparations, contractures to exogenous acetylcholine (55 and 110 microM) or KCl (13.4 mM) were unaltered after complete blockade by all toxin concentrations. These results, which strongly suggested a presynaptic mechanism of action for this toxin, were reinforced by (1) the inability of BaTX to interfere with the carbachol-induced depolarization of the resting membrane, (2) a significant decrease in the frequency and amplitude of miniature end-plate potentials, and (3) a significant reduction (59+/-4%, n=12) in the quantal content of the end-plate potentials after a 60 min incubation with the toxin (1.4 microM). In addition, a decrease in the organ bath temperature from 37 degrees C to 24 degrees C and/or the replacement of calcium with strontium prevented the neuromuscular blockade, indicating a temperature-dependent effect possibly mediated by enzymatic activity.
Asunto(s)
Bothrops , Venenos de Crotálidos/enzimología , Bloqueantes Neuromusculares/farmacología , Unión Neuromuscular/efectos de los fármacos , Fosfolipasas A2/farmacología , Terminales Presinápticos/efectos de los fármacos , Animales , Calcio/química , Embrión de Pollo , Agonistas Colinérgicos/farmacología , Cromatografía Líquida de Alta Presión , Venenos de Crotálidos/química , Venenos de Crotálidos/aislamiento & purificación , Venenos de Crotálidos/farmacología , Diafragma/efectos de los fármacos , Diafragma/inervación , Relación Dosis-Respuesta a Droga , Estimulación Eléctrica , Electroforesis en Gel de Poliacrilamida , Masculino , Ratones , Potenciales Postsinápticos Miniatura , Peso Molecular , Bloqueantes Neuromusculares/química , Bloqueantes Neuromusculares/aislamiento & purificación , Fosfolipasas A2/química , Fosfolipasas A2/aislamiento & purificación , Nervio Frénico/efectos de los fármacos , Análisis de Secuencia de Proteína , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Transmisión Sináptica/efectos de los fármacos , Temperatura , Factores de TiempoRESUMEN
We report here the first six cases of leprosy associated with HLA-identical allogeneic SCT in different phases and with different findings and outcomes. Skin and peripheral nerves may be sites of leprosy associated with SCT, stressing the importance of differential diagnosis between leprosy and GVHD or drug reactions. Clinical manifestations of leprosy before or after transplantation did not influence the outcome of SCT in our cases.
Asunto(s)
Trasplante de Células Madre Hematopoyéticas/efectos adversos , Lepra/etiología , Adulto , Diagnóstico Diferencial , Femenino , Humanos , Lepra/diagnóstico , Lepra/patología , Masculino , Enfermedades del Sistema Nervioso Periférico/diagnóstico , Enfermedades del Sistema Nervioso Periférico/etiología , Enfermedades de la Piel/diagnóstico , Enfermedades de la Piel/etiología , Trasplante Homólogo , Resultado del TratamientoRESUMEN
The intestinal microbiota, a barrier to the establishment of pathogenic bacteria, is also an important reservoir of opportunistic pathogens. It plays a key role in the process of resistance-genes dissemination, commonly carried by specialized genetic elements, like plasmids, phages, and conjugative transposons. We obtained from strains of enterobacteria, isolated from faeces of newborns in a university hospital nursery, indication of phenotypical gentamicin resistance amplification (frequencies of 10(-3) to 10(-5), compatible with transposition frequencies). Southern blotting assays showed strong hybridization signals for both plasmidial and chromosomal regions in DNA extracted from variants selected at high gentamicin concentrations, using as a probe a labeled cloned insert containing aminoglycoside modifying enzyme (AME) gene sequence originated from a plasmid of a Klebsiella pneumoniae strain previously isolated in the same hospital. Further, we found indications of inactivation to other resistance genes in variants selected under similar conditions, as well as, indications of co-amplification of other AME markers (amikacin). Since the intestinal environment is a scenario of selective processes due to the therapeutic and prophylactic use of antimicrobial agents, the processes of amplification of low level antimicrobial resistance (not usually detected or sought by common methods used for antibiotic resistance surveillance) might compromise the effectiveness of antibiotic chemotherapy.
Asunto(s)
Heces/microbiología , Gentamicinas/farmacología , Klebsiella pneumoniae/efectos de los fármacos , Farmacorresistencia Microbiana/genética , Amplificación de Genes , Hospitalización , Humanos , Recién Nacido , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/aislamiento & purificación , Células MadreRESUMEN
Since the identification of phylloxera in Brazil, the search for adequate rootstocks became important in viticultural research activities in the State of São Paulo. Increased actions on breeding and selection, led to a reasonable number of grape rootstocks combining a number of advantageous characteristics. From these, however, only a few are used in the major areas of grape production. The expansion of viticulture to different regions has increased the need of new rootstocks to attend diversification. The aim of this research was to evaluate grapevine hybrids growing at the Centro Experimental de Campinas (Instituto Agronômico - IAC, 23o08'S and 46o55'W), with respect to their potential as new rootstocks. Twenty nine hybrids were selected for vigor and vegetative exuberance. These were compared with four rootstocks commonly used in vineyards of São Paulo: IAC 766, IAC 572, Ripária do Traviú and Kober 5BB. Cuttings were planted in outdoor conditions, in plastic bags with a mixture of soil, superphosphate and castor bean meal. Five trials were carried out to study the initial budding velocity, rooting (percentage of rooted cuttings, root length and dry weight), and vegetative development (shoot dry weight). The following groups of hybrids were selected: a) quick-budding and best rooting: 183-16 (Black July X IAC 21-14), 204-5 (Niagara Rosada X IAC 544-14) and 202-2 (Niagara Rosada X Vitis rupestris S. George); b) best rooting and best vegetative development: 202-21, 202-12 (Niagara Rosada X Vitis rupestris S. George), and 224-10 (Niagara Rosada X IAC 584-53); c) best performance in the three characteristics: 237-24 (Niagara Rosada X Seibel 1077), 202-6, 202-18, 202-1, 202-10, 202-7 e 202-9, (Niagara Rosada X Vitis rupestris S. George).
A expansão da viticultura e sua difusão em regiões que apresentam diversidade de condições, bem como a possível utilização de cultivares que apresentem diferentes níveis de afinidade, torna indispensável a viabilização de novos porta-enxertos que possam atender a essas finalidades. O objetivo deste trabalho foi avaliar genótipos resultantes de hibridações efetuadas no Instituto Agronômico de Campinas (IAC), quanto à possibilidade de se tornarem novos porta-enxertos para videira. O trabalho foi conduzido na Seção de Viticultura do IAC, localizada no Centro Experimental de Campinas, SP. Foram utilizados 29 genótipos resultantes de cruzamentos realizados entre 1983 e 1985, escolhidos pelo seu vigor e exuberância vegetativa. Estes genótipos foram comparados com quatro porta-enxertos comumente utilizados nas regiões vitícolas do Estado de São Paulo: IAC 766, IAC 572, Ripária do Traviú e Kober 5BB. As estacas foram plantadas, ao ar livre, em sacos de polietileno contendo substrato composto de solo, superfosfato simples e torta de mamona. Foram implantados cinco experimentos e observados os seguintes parâmetros: velocidade de brotação, enraizamento (percentagem de estacas enraizadas, comprimento e peso da matéria seca de raízes) e desenvolvimento vegetativo (peso da matéria seca da parte aérea). Os resultados obtidos permitiram destacar os seguintes grupos de genótipos: a) os que apresentaram maior velocidade de brotação e melhor enraizamento: 183-16 (Black July X IAC 21-14), 204-5 (Niagara Rosada X IAC 544-14) e 202-2 (Niagara Rosada X Vitis rupestris S. George); b) os que apresentaram melhor enraizamento e melhor desenvolvimento vegetativo: 202-21, 202-12 (Niagara Rosada X Vitis rupestris S. George) e 224-10 (Niagara Rosada X IAC 584-53); c) aqueles que demonstraram melhor desempenho nas três características, consideradas conjuntamente: 237-24 (Niagara Rosada X Seibel 1077), 202-6, 202-18, 202-1, 202-10, 202-7 e 202-9 (Niagara Rosada X Vitis rupestris S. George).
RESUMEN
Since the identification of phylloxera in Brazil, the search for adequate rootstocks became important in viticultural research activities in the State of São Paulo. Increased actions on breeding and selection, led to a reasonable number of grape rootstocks combining a number of advantageous characteristics. From these, however, only a few are used in the major areas of grape production. The expansion of viticulture to different regions has increased the need of new rootstocks to attend diversification. The aim of this research was to evaluate grapevine hybrids growing at the Centro Experimental de Campinas (Instituto Agronômico - IAC, 23o08'S and 46o55'W), with respect to their potential as new rootstocks. Twenty nine hybrids were selected for vigor and vegetative exuberance. These were compared with four rootstocks commonly used in vineyards of São Paulo: IAC 766, IAC 572, Ripária do Traviú and Kober 5BB. Cuttings were planted in outdoor conditions, in plastic bags with a mixture of soil, superphosphate and castor bean meal. Five trials were carried out to study the initial budding velocity, rooting (percentage of rooted cuttings, root length and dry weight), and vegetative development (shoot dry weight). The following groups of hybrids were selected: a) quick-budding and best rooting: 183-16 (Black July X IAC 21-14), 204-5 (Niagara Rosada X IAC 544-14) and 202-2 (Niagara Rosada X Vitis rupestris S. George); b) best rooting and best vegetative development: 202-21, 202-12 (Niagara Rosada X Vitis rupestris S. George), and 224-10 (Niagara Rosada X IAC 584-53); c) best performance in the three characteristics: 237-24 (Niagara Rosada X Seibel 1077), 202-6, 202-18, 202-1, 202-10, 202-7 e 202-9, (Niagara Rosada X Vitis rupestris S. George).
A expansão da viticultura e sua difusão em regiões que apresentam diversidade de condições, bem como a possível utilização de cultivares que apresentem diferentes níveis de afinidade, torna indispensável a viabilização de novos porta-enxertos que possam atender a essas finalidades. O objetivo deste trabalho foi avaliar genótipos resultantes de hibridações efetuadas no Instituto Agronômico de Campinas (IAC), quanto à possibilidade de se tornarem novos porta-enxertos para videira. O trabalho foi conduzido na Seção de Viticultura do IAC, localizada no Centro Experimental de Campinas, SP. Foram utilizados 29 genótipos resultantes de cruzamentos realizados entre 1983 e 1985, escolhidos pelo seu vigor e exuberância vegetativa. Estes genótipos foram comparados com quatro porta-enxertos comumente utilizados nas regiões vitícolas do Estado de São Paulo: IAC 766, IAC 572, Ripária do Traviú e Kober 5BB. As estacas foram plantadas, ao ar livre, em sacos de polietileno contendo substrato composto de solo, superfosfato simples e torta de mamona. Foram implantados cinco experimentos e observados os seguintes parâmetros: velocidade de brotação, enraizamento (percentagem de estacas enraizadas, comprimento e peso da matéria seca de raízes) e desenvolvimento vegetativo (peso da matéria seca da parte aérea). Os resultados obtidos permitiram destacar os seguintes grupos de genótipos: a) os que apresentaram maior velocidade de brotação e melhor enraizamento: 183-16 (Black July X IAC 21-14), 204-5 (Niagara Rosada X IAC 544-14) e 202-2 (Niagara Rosada X Vitis rupestris S. George); b) os que apresentaram melhor enraizamento e melhor desenvolvimento vegetativo: 202-21, 202-12 (Niagara Rosada X Vitis rupestris S. George) e 224-10 (Niagara Rosada X IAC 584-53); c) aqueles que demonstraram melhor desempenho nas três características, consideradas conjuntamente: 237-24 (Niagara Rosada X Seibel 1077), 202-6, 202-18, 202-1, 202-10, 202-7 e 202-9 (Niagara Rosada X Vitis rupestris S. George).
RESUMEN
Two packing types and exponsure to ethylene, in room and refrigerator conditions, were studied for fruits of sweet pepper Agronomico 10G. After 5 day intervals the fruits were weighed. It was concluded that the best temperature range for sweet pepper preservation is 7 to 9°C; at these temperatures, the best packaging was aluminium trays covered with 0,0165 mm PVC film. Potassium permanganate in perforated capsules showed no evident effect as ethylene absorbent. After 30 days fruit losses were very high due to senescence and/or disease incidence.
Com o objetivo de verificar a conservação de frutos de pimentão, foi estudada a utilização de dois tipos de embalagem e um seqüestrador de etileno, em condições ambientais e em geladeira. As avaliações foram realizadas a cada cinco dias, obtendo-se as seguintes conclusões: a melhor temperatura para conservação de pimentões situa-se entre 7 e 9°C; o armazenamento de pimentões em bandejas envolvidas com filme de PVC, constitui-se na melhor alternativa para sua conservação, naquela faixa de temperatura. O permanganato de potássio, colocado em cápsulas perfuradas no interior das embalagens, não apresentou efeito evidente como absorvente de etileno. Após 30 dias, foram verificadas perdas de frutos por senescência e/ou pela incidência de doenças.
RESUMEN
Two packing types and exponsure to ethylene, in room and refrigerator conditions, were studied for fruits of sweet pepper Agronomico 10G. After 5 day intervals the fruits were weighed. It was concluded that the best temperature range for sweet pepper preservation is 7 to 9°C; at these temperatures, the best packaging was aluminium trays covered with 0,0165 mm PVC film. Potassium permanganate in perforated capsules showed no evident effect as ethylene absorbent. After 30 days fruit losses were very high due to senescence and/or disease incidence.
Com o objetivo de verificar a conservação de frutos de pimentão, foi estudada a utilização de dois tipos de embalagem e um seqüestrador de etileno, em condições ambientais e em geladeira. As avaliações foram realizadas a cada cinco dias, obtendo-se as seguintes conclusões: a melhor temperatura para conservação de pimentões situa-se entre 7 e 9°C; o armazenamento de pimentões em bandejas envolvidas com filme de PVC, constitui-se na melhor alternativa para sua conservação, naquela faixa de temperatura. O permanganato de potássio, colocado em cápsulas perfuradas no interior das embalagens, não apresentou efeito evidente como absorvente de etileno. Após 30 dias, foram verificadas perdas de frutos por senescência e/ou pela incidência de doenças.
RESUMEN
Two clinical cases of acute respiratory failure during post-partum are presented. Clinical diagnostic, procedures and lab methods are emphasized. The rise of this pathology in the pregnant woman is pointed out.
Asunto(s)
Complicaciones del Trabajo de Parto/diagnóstico , Complicaciones del Embarazo/diagnóstico , Trastornos Puerperales/diagnóstico , Insuficiencia Respiratoria/diagnóstico , Enfermedad Aguda , Adulto , Cesárea , Femenino , Humanos , EmbarazoRESUMEN
Durante um periodo de dois anos foram estudados, de maneira prospectiva, as malformacoes congenitas passiveis de diagnostico pelo exame clinico no bercario em uma populacao de 14.020 nascidos vivos. Encontrou-se nessa populacao 235 recem-nascidos com malformacoes (1,67%). Essa incidencia aproxima-se muito da de dados publicados em 1966 para a cidade de Sao Paulo e e ligeiramente inferior a de varias outras publicacoes nacionais e estrangeiras. Foi estudada a correlacao de malformacoes com idade dos pais, idade gestacional, usos de raios X durante a gestacao, presenca de malformacao na familia, paridade, existencia de abortos espontaneos e induzidos, historia de natimortos, drogas durante a gestacao e doencas e intercorrencias incidindo no 1o trimestre da gravidez. Foi tambem estudada a distribuicao sazonal das malformacoes e a distribuicao geografica do domicilio de 141 maes com filhos malformados e de igual numero de controles com filhos normais