RESUMEN

Studies on sensory information processing typically focus on whisker-related tactile information, overlooking the question of how sensory inputs from other body areas are processed at cortical levels. Here, we present a protocol for stimulating specific rodent limb receptive fields while recording in vivo somatosensory-evoked activity. We describe steps for localizing cortical-hindlimb coordinates using acute peripheral stimulation, electrode placement, and the application of electrical stimulation. This protocol overcomes the challenge of inducing a reproducible and consistent stimulation of specific limbs. For complete details on the use and execution of this protocol, please refer to Miguel-Quesada et al.1.

Asunto(s)

Estimulación Eléctrica , Potenciales Evocados Somatosensoriales , Corteza Somatosensorial , Animales , Potenciales Evocados Somatosensoriales/fisiología , Estimulación Eléctrica/métodos , Corteza Somatosensorial/fisiología , Ratas , Ratones , Extremidades/fisiología , Roedores , Miembro Posterior/fisiología , Vibrisas/fisiología