RESUMEN
Following the works of Velazquez et al. (1999), Jo-Seung et al. (2000), Wang et al. (2001), Danscher et al. (2001) and the criteria of Zinc-containing neurons established by Frederickson et al.(2000), we have found the presence and localisation of Zinc in the neurons of the dorsal root ganglia of Wistar rat, by using Timm's thecnique and by studying the autoradiographic uptake of 65Zn. The agreement between the results of both techniques allows us to classify these spinal ganglion neurons as Zinc-containing neurons and also, to confirm some of the results of Velazquez et al. (1999).
Asunto(s)
Ganglios Espinales/metabolismo , Radioisótopos de Zinc/metabolismo , Zinc/metabolismo , Animales , Inmunohistoquímica , Ratas , Ratas WistarRESUMEN
OBJECTIVE: The purpose of this study was to analyze the lipid profiles in children six years of age according to sex and to compare this with other Spanish studies. PATIENTS AND METHODS: A transverse epidemiological study was performed. All six year old children in our area were studied. The following tests were performed in all children: total cholesterol (TC), triglycerides, LDL-C, HDL-C, apoproteins A and B after fasting for ten hours. In addition, TC/HDL-C, LDL-C/HDL-C, and Apo B/A ratios and atherogenic index (ATI) were calculated. RESULTS: A population of 673 subjects (352 males and 321 females) were studied. The value of TC, triglycerides, LDL-C, Apo B, LDL-C/HDL-C and TC/HDL-C were significantly greater in females compared to males. Males had higher levels of HDL-C and ApoA. The values were similar to those obtain in Madrid in 1992, except that the values of HL-C were significantly higher in both sexes. CONCLUSIONS: In six year old children, the lipid profile in girls is worse than in boys. The data from our study do not show that the lipidic risk is getting worse during the past years in Spanish children.
Asunto(s)
Apolipoproteínas A/sangre , Apolipoproteínas B/sangre , Colesterol/sangre , Lípidos/sangre , Arteriosclerosis/prevención & control , Niño , Estudios Transversales , Dieta Aterogénica , Estudios Epidemiológicos , Femenino , Humanos , Masculino , EspañaRESUMEN
Zinc has been located using both histochemical and autoradiographic procedures in the neurons of the nuclei of the hypothalamic medial area and in some adenohypophisary cells. Some suggestions about the functional significance of the presence of Zn in these places are made.
Asunto(s)
Sistema Hipotálamo-Hipofisario/metabolismo , Zinc/metabolismo , Animales , Autorradiografía , Histocitoquímica , Sistema Hipotálamo-Hipofisario/citología , Ratas , Ratas Wistar , Radioisótopos de ZincRESUMEN
Using histochemical and autoradiographical techniques the location of Zn in the hyaline cartilage of the Wistar rat was studied. A triple location was observed in the territorial matrix; in the chondrocyte; and in the perichondrio (cellular area). We believe that the molecules which were observed carrying Zn could be alkaline phosphatase, timidin-kinase and chondroitin-sulphuric acid.
Asunto(s)
Cartílago/metabolismo , Matriz Extracelular/metabolismo , Zinc/metabolismo , Animales , Autorradiografía , Cartílago/citología , Femenino , Histocitoquímica , Masculino , Ratas , Ratas Endogámicas , CostillasRESUMEN
We studied the location of Zn in the testicle of the Wistar rat using the Timm histochemical technique, autoradiography with 65Zn and the dynamic pathway of 65Zn in the organ. Locations of Zn in several cellular components of the spermatogenic chain and in the Leydig cells were observed. This location was proved statistically by a quantitative analysis of the autoradiographs. The presence of Zn in the testicle, prostate and the adrenal gland could be related to the activation of the enzyme beta-hydroxy-steroid dehydrogenase although this fact does not exclude any other function of this metal, as far as we know.