RESUMEN
Activation of ATP-sensitive potassium (KATP) channels present on vascular smooth muscle cells causes membrane hyperpolarization and vasodilation. The purpose of this study was to determine whether KATP channels contribute to reactive hyperemia in humans. Accordingly, we studied the effect of tolbutamide, a KATP channel inhibitor, on reactive hyperemic forearm blood flow. Forearm blood flow was measured by venous occlusion plethysmography. Forearm ischemia was produced by inflating a sphygmomanometric cuff on the arm to suprasystolic pressures for 5 min. After cuff release, forearm blood flow was measured during the reactive hyperemic phase for 5 min. Tolbutamide (1 mM blood concentration, n = 6) did not affect basal (2.4 +/- 0.2 to 2.2 +/- 0.1 ml.100 ml-1.min-1) or peak reactive hyperemic forearm blood flow (21.9 +/- 3.8 to 22.6 +/- 2.9 ml.100 ml-1.min-1, each P = NS), but it significantly attenuated total hyperemic volume (12.6 +/- 1.7 vs. 9.2 +/- 1.8 ml/100 ml, P < 0.02). Vehicle (n = 6) did not affect basal flow, peak reactive hyperemic flow, or total hyperemia. To determine whether adenosine or endothelium-derived nitric oxide contribute to reactive hyperemia via KATP channels, adenosine (1.5-500 micro grams/min, n = 6) and acetylcholine (30 micrograms/min, n = 6) were infused before and during tolbutamide coinfusion. Tolbutamide did not significantly alter the forearm blood flow response to either adenosine or acetylcholine. In conclusion, KATP channels contribute to vasodilation during reactive hyperemia in humans.
Asunto(s)
Adenosina Trifosfato/farmacología , Hiperemia/fisiopatología , Canales de Potasio/efectos de los fármacos , Canales de Potasio/fisiología , Acetilcolina/farmacología , Adenosina/farmacología , Adulto , Femenino , Antebrazo/irrigación sanguínea , Humanos , Masculino , Persona de Mediana Edad , Flujo Sanguíneo Regional/efectos de los fármacos , Tolbutamida/farmacología , Vasodilatadores/farmacologíaRESUMEN
OBJECTIVE: The aim was to examine agonist induced and myogenic venular responses after crystalloid cardioplegia in conditions simulating cardiopulmonary bypass. METHODS: Hearts of pigs were arrested with cold hyperkalaemic ([K+] = 25 mM) crystalloid cardioplegic solution for 1 h under conditions of cardiopulmonary bypass. In another group, hearts were arrested and then reperfused with warm blood for 1 h while being separated from cardiopulmonary bypass. In a third group, animals were supported on cardiopulmonary bypass for 75 min without diversion of coronary blood flow. Hearts from non-instrumented animals served as controls. Coronary venules (91-197 microns in internal diameter) were studied in vitro in a pressurised no flow state using video microscopy. Agonist induced responses were assessed in vessels precontracted with the thromboxane A2 analogue U46619. RESULTS: Endothelium dependent relaxations to adenosine diphosphate (ADP, P = 0.11 v control), or serotonin (P = 0.67), and endothelium independent relaxations to the beta adrenergic cyclic AMP mediated agonist isoprenaline (P = 0.20), adenosine (P = 0.98), or the KATP channel opener pinacidil (P = 0.40) were not significantly altered after cold cardioplegia alone. After cardioplegia followed by 1 h of warm blood reperfusion, venular responses to ADP (P = 0.003 v control), isoprenaline (P = 0.013), adenosine (P = < 0.001), and pinacidil (P = 0.005) were reduced compared to the respective control responses, while the response to serotonin (P = 0.97) remained unchanged. Endothelium independent cyclic GMP mediated relaxation to sodium nitroprusside was similar in all groups (P > 0.90). Myogenic reactivity was assessed after incremental increases in the intraluminal pressure from 2-40 mm Hg. As intraluminal pressure was increased, the diameter of control venules increased and reached a plateau. Following cardioplegia, the pressure-diameter relationship of venules was shifted upward (P = 0.04 v control) suggesting impaired myogenic tone. After reperfusion, myogenic tone partially recovered. Extracorporeal circulation without diversion of coronary perfusion did not significantly affect venular responses. CONCLUSIONS: Ischaemic cardioplegia using a cold hyperkalaemic solution under conditions of cardiopulmonary bypass does not significantly alter agonist induced venular responses, whereas myogenic contraction is slightly reduced. After 1 h of reperfusion, agonist induced relaxations of coronary venules are significantly impaired, whereas myogenic contraction recovers. These findings may have implications for the control of myocardial perfusion and diastolic properties of the heart after ischaemic cardioplegia under conditions of extracorporeal circulation.
Asunto(s)
Agonistas Adrenérgicos beta/farmacología , Puente Cardiopulmonar , Vasos Coronarios/efectos de los fármacos , Paro Cardíaco Inducido , Músculo Liso Vascular/efectos de los fármacos , Vasodilatadores/farmacología , Adenosina/farmacología , Adenosina Difosfato/farmacología , Animales , Endotelio Vascular/efectos de los fármacos , Femenino , Guanidinas/farmacología , Isoproterenol/farmacología , Masculino , Nitroprusiato/farmacología , Pinacidilo , Serotonina/farmacología , PorcinosRESUMEN
RATIONALE AND OBJECTIVES: Iodinated contrast media can cause a number of well-described acute hemodynamic and vascular effects including vascular spasm, hypotension, and arrhythmias. Coronary microvessels were studied in vitro after high-dose exposure to an ionic, high-osmolar contrast agent diatrizoate meglumine in vivo. The aim of this study was to examine the endothelium-dependent and endothelium-independent vasodilator responses of the microvessels after previous contrast media administration in a clinically relevant setting. METHODS: Left coronary angiography was performed on six pigs using a cumulative dose of 60 mL (5 mL/injection) of diatrizoate meglumine. After 1 hour of reperfusion, epicardial coronary microvessels were studied in vitro in a pressurized, no-flow state with video microscopy. The vasodilators bradykinin, calcium ionophore A23187, and sodium nitroprusside were sequentially applied extraluminally after preconstriction. Serotonin and the thromboxane A2 analog U46619 were studied without preconstriction. RESULTS: Microvessels exposed to diatrizoate meglumine had normal relaxation responses to the endothelium-dependent vasodilators bradykinin and calcium ionophore A23187 when compared to control vessels. The vasoconstrictor responses to U46619 and serotonin were not significantly altered compared to control vessels. Responses to the endothelium-independent vasodilator sodium nitroprusside were not reduced or were slightly enhanced after exposure to contrast media. CONCLUSION: Coronary resistance vessels responses to the endothelium-dependent vasodilators bradykinin and calcium ionophore A23187 are not diminished after previous exposure to diatrizoate meglumine. The vasoconstrictor responses to U46619 and serotonin were similarly unaffected by previous exposure to contrast media. This suggests that, when used in clinically relevant amounts, diatrizoate meglumine does not cause functional endothelium or vascular smooth muscle impairment.
Asunto(s)
Vasos Coronarios/efectos de los fármacos , Diatrizoato de Meglumina/farmacología , Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico , Animales , Bradiquinina/farmacología , Calcimicina/farmacología , Endotelio Vascular/efectos de los fármacos , Femenino , Masculino , Microcirculación/efectos de los fármacos , Músculo Liso Vascular/efectos de los fármacos , Nitroprusiato/farmacología , Endoperóxidos de Prostaglandinas Sintéticos/farmacología , Serotonina/farmacología , Porcinos , Tromboxano A2/análogos & derivados , Tromboxano A2/farmacología , Resistencia Vascular/efectos de los fármacos , Vasoconstrictores/farmacología , Vasodilatadores/farmacologíaRESUMEN
Alterations of vascular reactivity may be a cause of reduced myocardial perfusion after cardioplegic arrest. The effects of blood and albumin cardioplegia on endothelium-dependent coronary microvascular function and ultrastructure were examined after cardiopulmonary bypass, ischemic arrest, and reperfusion. During cardiopulmonary bypass, porcine hearts were arrested with either blood, albumin-crystalloid, or crystalloid cardioplegia for 1 hour, followed with reperfusion for 1 hour. Noninstrumented pigs were used as controls. Coronary microarterial vessels (90 to 190 microns in diameter) were studied in a pressurized, no-flow state with video microscopic imaging and electronic dimension analysis. Ischemic arrest with crystalloid cardioplegia markedly reduced endothelium-dependent relaxations to the adenine nucleotide adenosine diphosphate and the calcium ionophore A23187. Enhanced contractile responses were observed to the platelet-derived vasoactive substance serotonin and to the thromboxane A2 analogue U46619. Indomethacin corrected the enhanced contractile responses to serotonin, indicating the enhanced release of a constrictor prostanoid substance. Indomethacin had no effect on the impaired relaxations to adenosine diphosphate or A23187. Endothelium-dependent relaxations to adenosine diphosphate, serotonin, and A23187 were significantly preserved with either blood or albumin-crystalloid cardioplegia, whereas contractile responses to U46619 were normal. Endothelium-independent relaxation to nitroprusside was similar in all groups, indicating normal smooth muscle responsiveness. Electron microscopy revealed minimal alterations of vascular morphology of vessels in both crystalloid and blood cardioplegia groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Albúminas , Sangre , Vasos Coronarios/fisiología , Endotelio Vascular/fisiología , Paro Cardíaco Inducido/métodos , Adenosina Difosfato/farmacología , Aminoácido Oxidorreductasas/antagonistas & inhibidores , Animales , Presión Sanguínea , Calcimicina/farmacología , Vasos Coronarios/efectos de los fármacos , Vasos Coronarios/ultraestructura , Inhibidores de la Ciclooxigenasa/farmacología , Endotelio Vascular/efectos de los fármacos , Endotelio Vascular/ultraestructura , Microcirculación , Microscopía Electrónica , Músculo Liso Vascular/efectos de los fármacos , Músculo Liso Vascular/fisiología , Reperfusión Miocárdica , Óxido Nítrico Sintasa , Serotonina/farmacología , Porcinos , Vasoconstricción/fisiología , Vasodilatación/fisiologíaRESUMEN
Coronary arteriolar spasm may occur following cardiopulmonary bypass and ischemic arrest, resulting in impaired cardiac function. Because myocardial perfusion is principally regulated by the microcirculation, the in vitro effects of various clinically used vasodilating drugs on porcine coronary microvessels less than 200 microns in diameter were examined following cardioplegic arrest and reperfusion. After 1 hour of ischemic arrest using either crystalloid or blood cardioplegia solutions followed by 1 hour of reperfusion, microvessels were studied in a pressurized (40 mmHg), no-flow state, and imaged with a video tracking device. Vessels were preconstricted by 30% to 60% of their resting diameter using acetylcholine, and various vasodilatory agents were applied extraluminally. Responses to the beta-adrenergic receptor agonist isoproterenol and the nitrovasodilator sodium nitroprusside were minimally altered by either cardioplegia solution as compared to control. In contrast, relaxation responses to both the calcium channel antagonist nifedipine and nitroglycerin were diminished after ischemic arrest and reperfusion. Relaxation responses were similar with crystalloid or blood cardioplegia for all drugs tested. Despite its somewhat attenuated response, nifedipine remained the most potent vasodilator of those studied. It is concluded that (1) following ischemic arrest with either crystalloid or blood cardioplegia solutions, responses to sodium nitroprusside and isoproterenol were minimally altered, while responses to nifedipine and nitroglycerin were attenuated; (2) relaxation responses of coronary arterioles were not significantly different with either blood or crystalloid cardioplegia; and (3) despite a slightly decreased response after cardioplegia, nifedipine was the most potent vasodilator of coronary arterioles, and may be the best choice for treating postoperative coronary arteriolar spasm.
Asunto(s)
Puente Cardiopulmonar , Vasos Coronarios/fisiología , Paro Cardíaco Inducido , Isoproterenol/farmacología , Nifedipino/farmacología , Nitroglicerina/farmacología , Nitroprusiato/farmacología , Compuestos de Potasio , Vasodilatación/fisiología , Animales , Sangre , Presión Sanguínea/fisiología , Soluciones Cardiopléjicas/uso terapéutico , Vasoespasmo Coronario/prevención & control , Vasos Coronarios/efectos de los fármacos , Vasos Coronarios/ultraestructura , Soluciones Hipertónicas/uso terapéutico , Microcirculación/efectos de los fármacos , Microcirculación/fisiología , Microcirculación/ultraestructura , Reperfusión Miocárdica , Complicaciones Posoperatorias/prevención & control , Potasio/uso terapéutico , Porcinos , Vasodilatación/efectos de los fármacosAsunto(s)
Bulimia/psicología , Cloruro de Potasio , Trastornos Relacionados con Sustancias/psicología , Adulto , Peso Corporal , Bulimia/complicaciones , Femenino , Humanos , Hiperpotasemia/inducido químicamente , Cloruro de Potasio/administración & dosificación , Cloruro de Potasio/efectos adversos , Automedicación , Trastornos Relacionados con Sustancias/complicacionesRESUMEN
STUDY OBJECTIVE: The aim was to investigate the role of the endothelium in modulating the acetylcholine response in porcine coronary microvessels and compare the results with simultaneously studied large coronary arteries. DESIGN: Coronary microvessels [104 (SEM 3.3) microns; range 38-150] were removed from fresh porcine hearts and studied in vitro during no flow constant pressure conditions. Endothelium derived relaxing factor (EDRF) activity and the role of the endothelium in modulating the acetylcholine response in microvessels was assessed by measuring changes in intraluminal diameter using a video tracking device. Large coronary arteries were simultaneously studied using conventional isometric ring techniques. EXPERIMENTAL MATERIAL: Fresh porcine hearts were obtained from a local slaughterhouse. MEASUREMENTS AND MAIN RESULTS: Acetylcholine was a potent vasoconstrictor (EC50 = 0.17 microM) of passively distended microvessels. The effects of EDRF were studied by either inactivation with haemoglobin or inhibition of EDRF synthesis with N-omega-nitro-L-arginine. Preconstricted microvessels exposed to either N-omega-nitro-L-arginine or haemoglobin constricted further, consistent with basal release of EDRF. Neither drug affected passively distended microvessels. The acetylcholine vasoconstrictor response was potentiated after exposure of microvessels to either drug. Atropine, but not indomethacin, blocked the acetylcholine response in microvessels. As with microvessels, acetylcholine was a vasoconstrictor (EC50 = 0.3 microM) of large coronary arteries. In contrast to microvessels, indomethacin antagonised acetylcholine vasoconstriction in vessels with intact endothelium. Bioassay experiments using indomethacin-treated large epicardial donor artery segments showed basal release of EDRF but no EDRF release in response to acetylcholine. CONCLUSIONS: The results show the microvessels and large coronary arteries are similar in their vasoconstrictor response to acetylcholine, that both release EDRF basally, and that vasoconstriction to acetylcholine is importantly modulated by the endothelium. In large arteries, acetylcholine does not stimulate EDRF release and, in contrast to microvessels, a cyclo-oxygenase product influences the vasoconstrictor action of acetylcholine.
Asunto(s)
Acetilcolina/farmacología , Vasos Coronarios/fisiología , Endotelio Vascular/fisiología , Vasoconstricción/efectos de los fármacos , Animales , Arginina/análogos & derivados , Arginina/farmacología , Vasos Coronarios/efectos de los fármacos , Hemoglobinas/farmacología , Técnicas In Vitro , Indometacina/farmacología , Microcirculación/efectos de los fármacos , Microcirculación/fisiología , Nitroarginina , Nitroprusiato/farmacología , PorcinosRESUMEN
Canine coronary resistance vessels were studied in vitro to examine the role of the endothelium in modulating responses to acetylcholine, vasopressin, and thrombin and to compare these responses to those found in large epicardial vessels. Acetylcholine had no effect on passively distended microvessels; however, after preconstriction with the thromboxane analogue, U 46619 caused dose-dependent vasodilation [50% effective concentration (EC50), 0.05 microM; maximum response, 97.9 +/- 2.1% relaxation]. Large epicardial arterial rings studied in organ chambers similarly relaxed to acetylcholine (EC50, 0.07 microM; maximum response, 79 +/- 5% relaxation). Hemoglobin was utilized to inactivate endothelium-derived relaxing factor (EDRF), resulting in reversal of acetylcholine vasodilation in both the microvessels (92 +/- 3.2% reversal) and the large epicardial vessels (117 +/- 9%). Hemoglobin had no effect on passively distended or preconstricted microvessels. Vasopressin constricted resistance vessels by 22.3 +/- 5.9 microns at 500 microU/ml. Hemoglobin potentiated this response by 100%, suggesting that vasopressin elicited EDRF release. In large coronary arteries, however, vasopressin elicited endothelium-dependent dilation with maximal relaxation of 36 +/- 9% at 3,000 microU/ml. Thrombin produced endothelium-dependent relaxation of large epicardial arterial rings but only constricted coronary microvessels. The response to thrombin was not altered by hemoglobin. This study demonstrates that the endothelium of coronary microvessels, like that of larger vessels, importantly modulates vascular reactivity to selected agents. Furthermore, major differences exist between large and small coronary arteries in their response to vasopressin and thrombin.
Asunto(s)
Vasos Coronarios/fisiología , Endotelio Vascular/fisiología , Músculo Liso Vascular/fisiología , Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico , Acetilcolina/farmacología , Animales , Atropina/farmacología , Factores Biológicos/farmacología , Presión Sanguínea/efectos de los fármacos , Vasos Coronarios/efectos de los fármacos , Vasos Coronarios/ultraestructura , Perros , Endotelio Vascular/ultraestructura , Femenino , Técnicas In Vitro , Indometacina/farmacología , Masculino , Microscopía Electrónica , Músculo Liso Vascular/efectos de los fármacos , Músculo Liso Vascular/ultraestructura , Óxido Nítrico , Potasio/farmacología , Endoperóxidos de Prostaglandinas Sintéticos/farmacología , Quinacrina/farmacología , Trombina/fisiología , Resistencia Vascular/efectos de los fármacos , Vasoconstricción , Vasodilatación/efectos de los fármacos , Vasopresinas/farmacologíaRESUMEN
A 40-year-old man with a history of renal stone formation was evaluated for left flank pain. An excretory urogram failed to show any renal calculi. However, renal arteriography and computerized tomography studies demonstrated a primary dissection of the left renal artery with resulting decreased perfusion to the lower pole of the left kidney.