RESUMEN
AIM: This study aimed to assess an ex situ model of biofilm-associated wounds on porcine skin for the study of Staphylococcus aureus and Pseudomonas aeruginosa biofilms in a host-like environment, after 48 to 120 h of incubation. MATERIAL AND RESULTS: Ex situ and in vitro biofilms were comparatively analysed. Overall, CFU-counts and matrix quantification yielded significantly (P < 0·05) higher results for ex situ than in vitro biofilms. Confocal microscopy revealed greater (P < 0·05) biomass and thickness at 48-72 h and greater (P < 0·05) robustness at 72 h of growth. S. aureus ex situ biofilms produced less (P < 0·05) siderophore and proteases than in vitro biofilms, while P. aeruginosa ex situ biofilms produced more (P < 0·05) siderophores and less proteases than in vitro biofilms. CONCLUSIONS: Biofilms grown ex situ present a greater amount of bacterial cells and polymeric matrix than their in vitro counterparts, reaching maturity at 72 h of growth. Moreover the production of virulence factors differs between ex situ and in vitro biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings emphasize the importance of using ex situ biofilm models, once they mimic in vivo conditions. The use of these models brings perspectives for the pursuit of therapeutic alternatives, as tests may be performed in a host-like environment.
Asunto(s)
Biopelículas , Pseudomonas aeruginosa , Piel/microbiología , Staphylococcus aureus , Animales , Técnicas In Vitro , Infecciones por Pseudomonas , Pseudomonas aeruginosa/crecimiento & desarrollo , Pseudomonas aeruginosa/patogenicidad , Infecciones Estafilocócicas , Staphylococcus aureus/crecimiento & desarrollo , Staphylococcus aureus/patogenicidad , Porcinos , Heridas y Lesiones/microbiologíaRESUMEN
Severely immunocompromised patients are at increased risk for uncommon infectious diseases with atypical presentations. Fusarium sp., has been reported in patients with hematological malignancies and prompt diagnosis is necessary due to high mortality. We report a myelodysplastic syndrome (MDS) patient who presented Fusarium solani infection associated with granulocytic sarcoma as an initial presentation of acute myeloid leukemia (AML) transformation. We performed histological examination, immunohistochemistry analysis, culture of the biopsy tissue and DNA sequencing to make a conclusive diagnosis of F. solani and granulocytic sarcoma, reinforcing the necessity of performing complete evaluation of skin lesions in immunocompromised patients.
Asunto(s)
Fusariosis/diagnóstico , Fusarium/aislamiento & purificación , Síndromes Mielodisplásicos/microbiología , Azacitidina/uso terapéutico , Biopsia , Diagnóstico Diferencial , Femenino , Fusarium/efectos de los fármacos , Fusarium/genética , Humanos , Huésped Inmunocomprometido , Leucemia Mieloide Aguda/complicaciones , Persona de Mediana Edad , Micelio/ultraestructura , Sarcoma Mieloide/diagnóstico , Sarcoma Mieloide/microbiología , Sarcoma Mieloide/radioterapia , Análisis de Secuencia de ADN , Piel/microbiología , Piel/patologíaRESUMEN
Dermatophytosis is the most common skin infectious disturbance in the world. In this research 2,297 patients were evaluated with suspected clinical lesions of dermatophytosis. It was observed that, 534 (23.2%) patients tested positive for dermatophytes. T. rubrum was the most prevalent specie (49.6%; p < or = 0.05), followed by T. tonsurans (34.4%), M. canis (7%) and T. mentagrophytes (6.2%). When the species isolated was correlated with the respective anatomical localization, it was observed that T. tonsurans was the most frequent isolated in scalp lesions (73.9%; p < or = 0.01). On the other hand, T. rubrum was the main specie involved in body lesions (72.8%; p < or = 0.05). Therefore, in scalp infections it was observed that, there was an absolute prevalence of T. tonsurans. This evidence is different from the statistical data collected in the southeast and south of Brazil, as well as from other areas of the world, which still show M. canis as the most frequent microorganism isolated in Tinea capitis.