RESUMEN
Fenoxaprop-p-ethyl (FE), 2-[4-[(6-chloro-2-benzoxazolyl)oxy]phenoxy] propanoate, ethyl ester (R), is an aryloxyphenoxypropionate herbicide for postemergence control of annual and perennial grasses in paddy fields; its site of action is acetyl-coenzyme A carboxylase (ACCase), an enzyme in fatty acids biosynthesis. The possible mechanism(s) of resistance to FE in a resistant biotype of Echinochloa phyllopogon was examined, namely, absorption, translocation, and metabolism of FE and ACCase susceptibility to fenoxaprop acid (FA). Studies of the in vitro inhibition of ACCase discounted any differential active site sensitivity as the basis of resistance to FE. There were differences in absorption rates between biotypes from 3 to 48 h after application (HAA). Biotypes did not differ in either the amounts or the rates of FE translocated; 98% of applied [14C]FE remaining in the treated leaf. However, there was a good correlation between the rate of herbicide metabolism and the plant resistance. The R biotype produced 5-fold less FA and approximately 2-fold more nontoxic (polar) metabolites 48 HAA than the S biotype. Moreover, the higher rate of GSH conjugation in the resistant biotype as compared to the susceptible one indicates that GSH and cysteine conjugation is the major mechanism of resistance of the R biotype against FE toxicity.
Asunto(s)
Echinochloa/efectos de los fármacos , Resistencia a los Herbicidas , Oxazoles , Propionatos , Absorción , Acetil-CoA Carboxilasa/antagonistas & inhibidores , Echinochloa/metabolismo , Inhibidores Enzimáticos/farmacología , Oxazoles/metabolismo , Oxazoles/farmacología , Propionatos/metabolismo , Propionatos/farmacologíaRESUMEN
The objective of this work was to evaluate the reliability of one quick tolerance test that would enable us, in only six days, to quantify tolerance levels of one biotype to one or more herbicides. For this purpose, we evaluated tolerance levels to cyhalofop-butyl of five Echinochloa spp. biotypes: muricata, crus-galli, crus-pavonis, oryzicola and utilis, with unknown records of herbicide treatment. Moreover, two biotypes of Echinochloa phyllopogon were tested, one of them resistant (R) to fenoxaprop-P-ethyl and the other one susceptible (S). The Oryza sativa Puntal var. and Oryza sativa var. (wild rice) were also checked in order to demonstrate cyhalofop-butyl selectivity profiles. The assays were conducted with petri-dishes where, over the following increasing doses, fifty seeds per biotype and dose, were placed in each one: 0, 3, 6, 12, 18, 24, 48 and 60 ppm of cyhalofop-butyl and codacide oil adjuvant. Six days later, the following parameters were evaluated: plumule length, root length, germination (%) and fresh weight reduction (%). E. muricata germination was reduced by 50% with a 6 ppm dose. A non linear relation dose-germination was observed in the rest of the biotypes. The plumule length permitted the ranking of the biotypes into three groups: (1) resistant (E. phyllopogon R) or very tolerant (Oryza sativa Puntal var. and wild rice), those with a reduction of less than 30%, (2) tolerant, between 30 and 60% (E. oryzicola), and (3) susceptible, with a reduction of over 80% (E. muricata, E. crus-galli, E. crus-pavonis, E. utilis and E. phyllopogon S). Only Oryzo sativa and E. phyllopogon R had a minimal reduction of fresh weight (less than 15%). The assays were repeated with whole plants and we checked the accuracy of this test that indicated the resistance level of one biotype in a quick, reliable and economic way.
Asunto(s)
Echinochloa/fisiología , Fungicidas Industriales/toxicidad , Herbicidas/toxicidad , Semillas/microbiología , Tolerancia a Medicamentos , Echinochloa/clasificación , Echinochloa/efectos de los fármacos , Oryza/microbiología , Control de Plagas/métodos , Raíces de Plantas/microbiologíaRESUMEN
A consequence of modern agriculture and its dependence on herbicides, is the appearance of resistant weed populations. The first case of resistance on the Iberian Peninsula was found in Portugal and it was in a population of Alisma plantago-aquatica, resistant to bensulfuronmethyl, an ALS-inhibitor. For the same herbicide, control problems with Cyperus difformis were found in some Spanish rice fields. In the same rice field area, tolerance was observed in some Echinochloa spp. when auxinic herbicides (quinclorac) were used and some resistant Echinochloa spp. populations were found due to the long use of PS II inhibitors herbicides (propanil). The association of active ingredients, rotation of crops and adoption of mechanical control methods are the main strategies to combat resistance to herbicides.