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1.
Iran J Vet Res ; 22(2): 140-145, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34306112

RESUMEN

BACKGROUND: Micro-computed tomography (µCT), a modern imaging technique, provides detailed information on the bone morphology of small animal models. AIMS: The objectives are 1) to produce three dimensional (3D) models from µCT images of femoral and tibial bones of New Zealand rabbits, and 2) to estimate and compare morphometric and volumetric results among genders as well as left and right sides. METHODS: A total of twenty adult New Zealand rabbits (10 females, 10 males, aged 12-18 weeks, weight= 2.5-3 kg) were used for this study. Three dimensional reconstructed models of the femoral and tibial bones of rabbits were created from cross-sectional images of µCT using the 3D Slicer program. Anatomical structures were determined on these 3D bone models. Afterward, morphometric parameters such as length, thickness, and width of various parts of the bones were calculated with volume and volume ratio values of cortical bone, trabecular bone, and medullary cavity. RESULTS: The gender*laterality interaction term was found statistically significant in measurements of femoral diaphysis diameter (FDD), internal femoral diaphysis diameter (IFDD), femoral head diameter (FHD), tibial diaphysis diameter (TDD), tibial distal width (TDH), and tibial proximal width (TPW) (P<0.001). The gender*laterality interaction term was not significant in volume and volume fraction values of cortical bone, trabecular bone, and medullary cavity (P>0.05). CONCLUSION: It is thought that the study will contribute to the orthopedic experimental studies of rabbits for femoral and tibial bones and will bring a modern perspective to the field of veterinary anatomy.

2.
Arq. bras. med. vet. zootec. (Online) ; 73(2): 320-326, Mar.-Apr. 2021. tab, ilus
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1248943

RESUMEN

In this study, fish's morphologic and anatomic lesions caused by motile aeromonad septicemia (MAS) depending on environmental stress in carp, Cyprinus carpio population living in Lake Tödürge were identified. Various morphological and anatomical deformations and lesions were observed in the body of approximately 17% (252 fish specimens) of a total of 1488 carp samples. Bacteria are grown from all wipe samples. Bacterial colonies have a gray-white appearance with round, convex and smooth edges. 15-20 cfu colonies were observed in each aerop culture. As a result of analysis of wet wipe samples from infected fish's skin, gill, kidney and liver, it is determined that the bacteria which causes septicemia is Aeromonas sobria from the Aeromonadaceae family (with 99.2% confidence value). No bacteria were grown in cultures except A. sobria. Some symptoms of the infection are inflammation on different parts of the fish bodies, eruption on skin and scales, dermal necrosis, degeneration at soft rays of the fins, exophthalmos, and purulent liquid accumulation in the abdominal cavity, etc. Infected fish were most commonly encountered in July and August (water temperature above 20ºC), the lowest in October and November (water temperature below 10ºC).(AU)


Neste estudo, foram identificadas lesões morfológicas e anatômicas causadas por septicemia móvel por aeromônios (MPA), dependendo do estresse ambiental da carpa, a população de Cyprinus carpio que vive no lago Tödürge foi identificada. Várias deformações e lesões morfológicas e anatômicas foram observadas no corpo de aproximadamente 17% (252 amostras de peixes) de um total de 1488 amostras de carpa. As bactérias são cultivadas a partir de todas as amostras de limpeza. As colônias bacterianas têm uma aparência branco-acinzentada, com bordas arredondadas, convexas e lisas. Foram observadas 15-20 colônias de UFC em cada cultura de aerop. Como resultado da análise de amostras de lenços umedecidos da pele, brânquias, rins e fígado de peixes infectados, é determinado que a bactéria que causa a septicemia é a Aeromonas sobria, da família Aeromonadaceae (com valor de confiança de 99,2%). Nenhuma bactéria foi cultivada em culturas, exceto A. sobria. Alguns sintomas da infecção são inflamação em diferentes partes dos corpos dos peixes, erupção na pele e escamas, necrose dérmica, degeneração aos raios moles das barbatanas, exoftalmia e acúmulo de líquido purulento na cavidade abdominal, entre outros. Os peixes infectados eram encontrados com maior frequência em julho e agosto (temperatura da água acima de 20ºC), e eram menos comumente encontrados em outubro e novembro (temperatura da água abaixo de 10ºC).(AU)


Asunto(s)
Animales , Cyprinidae/microbiología , Bacteriemia/veterinaria , Aeromonas/aislamiento & purificación , Estrés Fisiológico , Turquía
3.
Rheumatol Int ; 27(4): 335-8, 2007 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-17102942

RESUMEN

Leptin is an adypocyte derivated peptide hormone that plays a major role in preventing obesity development by the effects at the hypothalamic level. In our study leptin levels of 41 rheumatoid arthritis (RA) patients and 25 healthy subjects as control group were assessed. Synovial fluid from 21 RA patients were collected to detect leptin levels. Synovial fluid and plasma leptin levels were analysed and correlated with RA duration, ESR, CRP, X ray changes (erosive or non-erosive disease) and negative or positive test for rheumatoid factor. There wasn't any significant difference at plasma leptin levels between RA patients (3.91 +/- 6.15) and control group (4.94 +/- 6.44) (p > 0.05). Plasma leptin levels were correlated with body mass index (BMI) in both healthy subjects and RA patients (r = 0.37; p = 0.018). Therefore in RA patients, plasma and synovial fluid leptin levels were not correlated with disease duration, ESR, CRP, negative or positive test for rheumatoid factor and erosive or non-erosive disease (p > 0.05). In conclusion leptin is correlated with BMI both in RA patients and healthy individuals but no considerable relation with disease activity.


Asunto(s)
Artritis Reumatoide/sangre , Leptina/sangre , Líquido Sinovial/química , Adulto , Artritis Reumatoide/metabolismo , Biomarcadores , Índice de Masa Corporal , Estudios de Casos y Controles , Femenino , Humanos , Leptina/metabolismo , Masculino , Persona de Mediana Edad , Líquido Sinovial/metabolismo
5.
Am J Otolaryngol ; 26(1): 51-3, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-15635582

RESUMEN

OBJECTIVE: The purpose of this study is to compare transient bacteriemia ratios between cold dissection tonsillectomy and bipolar cauterizing tonsillectomy, and also to analyze the bacteria detected with superficial/central tonsillar cultures. METHODS: A total of 86 patients that were grouped as 46 patients of cold dissection tonsillectomy and 40 patients of bipolar cauterizing tonsillectomy were included in this study. Preoperative surface swab cultures, intraoperative central swab cultures of tonsils, and preoperative and postoperative blood culture samples were obtained. Antibiotic sensitivity tests were determined. Fischer exact chi 2 test was performed to compare the results of postoperative bacteriemia of both techniques statistically. RESULTS: Postoperative bacteriemia was detected in 6 (13%) patients of cold dissection tonsillectomy group. In 5 (83.3%) of the 6 postoperative bacteriemia patients of cold dissection tonsillectomy group, isolated microorganism was confirmed both in the blood cultures and in the central swab cultures of tonsils, and resistance to penicillin was established. No patient of bipolar cauterizing tonsillectomy group appeared with postoperative bacteriemia. There was a statistically significant difference (P=.028) for postoperative bacteriemia between cold dissection tonsillectomy group and bipolar cauterizing tonsillectomy group. CONCLUSION: We recommend bipolar cauterizing tonsillectomy for high-risk patients.


Asunto(s)
Bacteriemia/epidemiología , Tonsila Palatina/microbiología , Complicaciones Posoperatorias/epidemiología , Tonsilectomía/métodos , Tonsilitis/cirugía , Preescolar , Enfermedad Crónica , Criocirugía , Electrocoagulación , Femenino , Humanos , Masculino , Complicaciones Posoperatorias/microbiología , Recurrencia , Tonsilitis/microbiología , Resultado del Tratamiento
6.
Microb Drug Resist ; 10(2): 154-9, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15256031

RESUMEN

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial infections in our hospital. Therefore, we aimed to characterize MRSA isolates phenotypically from patients with nosocomial infections at Cumhuriyet University Hospital between December, 1999, and June, 2001, in Sivas by analysis of antibiotic patterns and genotypically using pulsed-field gel electrophoresis (PFGE) and repetitive element sequence-based polymerase chain reaction (rep-PCR). Forty-three nosocomial isolates were collected from various wards. All isolates were resistant to penicillin, tetracycline, oxacillin, and gentamicin. By rep-PCR and by separation of SmaI fragments of genomic DNA using PFGE, one major type (eight subtypes with PFGE) was identified among the strains. This clone was found to be different than some clones such as Iberian, Brazilian, and a major clone that was found in another Turkish University Hospital in Ankara. According to our results, there is a major MRSA clone with a potential to spread in our hospital. Infection control measures should be directed toward restricting the further spread of this clone. Therefore, in accordance with these findings, a surveillance culturing program should be established.


Asunto(s)
Resistencia a la Meticilina/genética , Staphylococcus aureus/genética , Técnicas de Tipificación Bacteriana , Infección Hospitalaria/epidemiología , Infección Hospitalaria/microbiología , Cartilla de ADN , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Farmacorresistencia Bacteriana/genética , Electroforesis en Gel de Campo Pulsado , Genotipo , Hospitales de Enseñanza , Humanos , Pruebas de Sensibilidad Microbiana , Reacción en Cadena de la Polimerasa , Infecciones Estafilocócicas/epidemiología , Staphylococcus aureus/clasificación , Staphylococcus aureus/aislamiento & purificación , Turquía/epidemiología
7.
Clin Exp Med ; 1(1): 61-6, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11467403

RESUMEN

Bacterial translocation is thought to be responsible for infectious complications after hemorrhagic shock. The aim of this study was to investigate the effects of pentoxifylline treatment on bacterial translocation in animals subjected to hemorrhagic shock. Thirty-one Wistar albino rats (280-360 g) were divided into three groups: sham (n=10), shock (n=11), and shock-pentoxifylline (n=10). Blood was not withdrawn from sham rats. Shock rats were subjected to 30 min of shock followed by reinfusion of shed blood. Shock/pentoxifylline rats received pentoxifylline after reinfusion of shed blood. After hemorrhage and reinfusion (24 h), the mesenteric lymph nodes, liver, spleen, and blood samples were evaluated using quantitative microbiological techniques, and the numbers of colony-forming units were compared between groups. Cecum was removed to evaluate the bacterial population. Ileum and cecum were examined histologically. The incidence of bacterial translocation was higher in the shocked rats (63%) than in the sham shock rats (10%). Pentoxifylline reduced the incidence of shock-induced bacterial translocation to 0%. Cecal bacterial levels were significantly higher in the shock rats than in the sham and shock/pentoxifylline rats. The histological damage caused by hemorrhagic shock was prevented by pentoxifylline treatment. In conclusion, the hemorrhagic shock triggered translocation of bacteria to the mesenteric lymph nodes, spleen, liver, and blood of rats. Pentoxifylline treatment just after shed blood transfusion significantly attenuated this phenomenon.


Asunto(s)
Traslocación Bacteriana/efectos de los fármacos , Mucosa Intestinal/patología , Choque Hemorrágico/fisiopatología , Animales , Infecciones Bacterianas/prevención & control , Traslocación Bacteriana/fisiología , Sangre/microbiología , Transfusión de Sangre Autóloga , Ciego , Citrobacter/aislamiento & purificación , Modelos Animales de Enfermedad , Enterococcus/aislamiento & purificación , Escherichia coli/aislamiento & purificación , Íleon , Mucosa Intestinal/efectos de los fármacos , Hígado/microbiología , Ganglios Linfáticos/microbiología , Masculino , Pentoxifilina , Proteus/aislamiento & purificación , Ratas , Ratas Wistar , Choque Hemorrágico/patología , Bazo/microbiología , Staphylococcus/aislamiento & purificación , Streptococcus/aislamiento & purificación
9.
J Trop Pediatr ; 41(6): 369-71, 1995 12.
Artículo en Inglés | MEDLINE | ID: mdl-8606448

RESUMEN

Serum ferritin, iron, and haemoglobin (Hb) values of 27 pregnant women who did not receive oral iron therapy during pregnancy, and Hb of their normal full-term babies were determined. Maternal blood samples were obtained at 16 and 24 weeks of pregnancy and infants' blood samples were obtained at the first day and 3 months of life. Mothers were divided into two groups according to their serum ferritin values. By analysing the results we were not able to detect any correlation between maternal ferritin, Hb, and newborn gestational age, and Hb and birth weight. The same was found when the groups were compared by a maternal serum ferritin above and below 12 ng/ml.


Asunto(s)
Anemia Ferropénica/complicaciones , Hemoglobinas/metabolismo , Recién Nacido/sangre , Complicaciones del Embarazo , Anemia Ferropénica/sangre , Femenino , Humanos , Lactante , Masculino , Pobreza , Embarazo , Turquía
10.
Clin Pediatr (Phila) ; 33(6): 349-52, 1994 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-8200169

RESUMEN

The purpose of this study was to determine the role of intravenous immunoglobulin (IVIG) administration in preterm neonates with S. typhimurium infection. A randomized trial of 47 preterm neonates with intestinal or extraintestinal S. typhimurium infection was performed. Neonates were randomly divided into two groups: 22 neonates were only given cefoperazone (group 1); 25 neonates were given cefoperazone plus IVIG (group 2). IVIG was given at a dose of 500 mg/kg on days 1, 2, 3, and 8 after entry into the study. Following treatment, bacteremia, complications, mortality rate, recovery time, and duration of antimicrobial therapy were evaluated in two groups. Bacteremia was found in 31.4% in group 1 and 8% in group 2 (P < .05); complications developed in 81.8% in group 1 and 16% in group 2 (P < 0.01); mortality was 40.9% in group 1 and 12% in group 2 (P < .05). Recovery took 15 days in group 1 and 8 days in group 2 (P < .01). The duration of antimicrobial therapy was 20 days in group 1 and 14 days in group 2 (P < .01). We conclude that IVIG treatment in combination with antibiotics in preterm neonates with S. typhimurium infection reduces the complications, mortality rate, and duration of therapy.


Asunto(s)
Inmunoglobulinas Intravenosas/uso terapéutico , Enfermedades del Prematuro/tratamiento farmacológico , Infecciones por Salmonella/tratamiento farmacológico , Salmonella typhimurium , Cefoperazona/administración & dosificación , Cefoperazona/uso terapéutico , Quimioterapia Combinada , Femenino , Humanos , Inmunoglobulinas Intravenosas/administración & dosificación , Recién Nacido , Recien Nacido Prematuro , Masculino
12.
Mikrobiyol Bul ; 27(4): 352-6, 1993 Oct.
Artículo en Turco | MEDLINE | ID: mdl-8264449

RESUMEN

Yeast infections play an important role in diabetics. Therefore poorly controlled diabetics and diabetics who have high levels of serum glucose carry a high risk. In the present study, 52 diabetics aged between 17-75 years old have been investigated by means of the incidence of oral candida and have been compared with control group of 33 healthy individuals. In poorly controlled diabetics, the rate of candida growth found high. It is also observed that this ratio is higher in type I than type II, and candida growth rate increased as well as serum glucose level. All of these results are found to be statistically important.


Asunto(s)
Candidiasis Bucal/etiología , Diabetes Mellitus Tipo 1/complicaciones , Diabetes Mellitus Tipo 2/complicaciones , Adolescente , Adulto , Anciano , Glucemia/análisis , Candidiasis Bucal/epidemiología , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Turquía/epidemiología
13.
Mikrobiyol Bul ; 27(3): 249-53, 1993 Jul.
Artículo en Turco | MEDLINE | ID: mdl-8361415

RESUMEN

In this study, the sera taken from 34 prostitutes, 34 women having spontaneous abortion and 34 control group were tested for C. trachomatis IgA and IgG antibodies. C. trachomatis antibodies were found positive in 30 (88.2%) of prostitutes, 11 (32.3%) of women having spontaneous abortion, 9 (26.4%) of controls. When the positivity rate of prostitutes and women having spontaneous abortion compared with control groups statistically, it was found significant in prostitutes (p < 0.05), but not found significant in women having spontaneous abortion (p > 0.05).


Asunto(s)
Aborto Espontáneo/microbiología , Chlamydia trachomatis/inmunología , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Trabajo Sexual , Adulto , Anticuerpos Antibacterianos/sangre , Femenino , Humanos , Embarazo
14.
Trop Geogr Med ; 42(3): 238-40, 1990 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-2293433

RESUMEN

In this study, 40 patients with diaper dermatitis and 20 healthy infants were investigated. Mycological and bacteriological cultures were obtained from affected and healthy skin areas and stool specimens. Candida albicans was isolated simultaneously in 37 out of 40 patients subjected to mycological examination of skin lesions and stool specimen cultures. Of 34 Gram-stained smears from the skin lesions, 27 revealed Candida in hyphal form. In the control group, C. albicans was not grown from the diaper area. No significant difference was observed in the growth of bacteria from the skin with or without lesions (p greater than 0.05). In conclusion, C. albicans-infection originating from the gastrointestinal tract, plays a major role in diaper dermatitis. Thus, a Gram-stained smear can be used as a practical method in early diagnosis.


Asunto(s)
Dermatitis del Pañal/microbiología , Heces/microbiología , Enfermedades Cutáneas Infecciosas/microbiología , Candida albicans/aislamiento & purificación , Humanos , Lactante , Recién Nacido , Micología/métodos
15.
Mikrobiyol Bul ; 24(3): 187-92, 1990 Jul.
Artículo en Turco | MEDLINE | ID: mdl-2283969

RESUMEN

The detection of bacteremia in children less than 24 months of age with fever, is very important, since these patients are at serious risk pyogenic complication. The administration of antibiotics in accordance with the results of cultures is too time consuming. So, several laboratory detection bacteremia must be carried out. In this study, blood cultures, the elevation of white blood cell count, toxic granulation and vacuolization in neutrophils were investigated in 56 children with suspicion of bacteremia. 32 of them were males and 24 females. The positive results were obtained in 41.1% in blood cultures. Positive predictive accuracy were 66.6% and 52.3% for vacuolization and toxic granulation respectively. The presence of these were considered as adjunctive procedures in the early diagnosis of bacteremia.


Asunto(s)
Neutrófilos/ultraestructura , Sepsis/diagnóstico , Gránulos Citoplasmáticos/ultraestructura , Femenino , Humanos , Lactante , Recuento de Leucocitos , Masculino , Valor Predictivo de las Pruebas , Sepsis/sangre , Vacuolas/ultraestructura
16.
Mikrobiyol Bul ; 22(1): 1-7, 1988 Jan.
Artículo en Turco | MEDLINE | ID: mdl-3078787

RESUMEN

The bacteria from the urine and renal biopsy specimens of 40 patients undergoing renal surgery were isolated and their relations with renal histology investigated. The urine cultures were positive in 14 patients, the same organisms being isolated from the renal tissue in 7 cases. In 6 patients with negative urine cultures, bacteria were isolated from renal tissues. Of the 28 cases pathologically diagnosed as chronic pyelonephritis, bacteria were isolated from the renal tissue in 13 cases, the urine cultures being positive in only 11 cases. E. coli was the most commonly encountered bacteria in both the urine and renal tissues.


Asunto(s)
Bacterias/aislamiento & purificación , Bacteriuria/microbiología , Riñón/microbiología , Pielonefritis/microbiología , Escherichia coli/aislamiento & purificación , Humanos , Riñón/patología
17.
Mikrobiyol Bul ; 20(2): 67-75, 1986 Apr.
Artículo en Turco | MEDLINE | ID: mdl-3773798

RESUMEN

In fifty newborn babies, gastric aspirate fluids obtained during the one-hour post partum period were examined from the stand point of PML and cultures. A susceptibility to infections (especially lung infections and sepsis) were established in 8 (30.8%) out of 26 cases with EMR more than twelve hours. PML counts of these cases were 13 and more than 13 per high power field. Significant differences were found between the babies with and without EMR, from the point-of-view of PML count in gastric aspirate fluid and the increased incidence of infections (p less than 0.05).


Asunto(s)
Infecciones Bacterianas/etiología , Rotura Prematura de Membranas Fetales/complicaciones , Líquidos Corporales/citología , Líquidos Corporales/microbiología , Femenino , Humanos , Recién Nacido , Recuento de Leucocitos , Neutrófilos , Embarazo , Infecciones del Sistema Respiratorio/etiología
18.
Mikrobiyol Bul ; 19(4): 235-7, 1985 Oct.
Artículo en Turco | MEDLINE | ID: mdl-3938520

RESUMEN

An outbreak of food poisoning was observed in Sivas on March 27, 1985 involving 25 patients who work at the same place. Clinical picture was characterized with nausea, abdominal pain and watery diarrhea. B. cereus was isolated from the stool of 4 patients. No other enteropathogens were found.


Asunto(s)
Bacillus cereus/aislamiento & purificación , Enfermedades Transmitidas por los Alimentos/microbiología , Diarrea/microbiología , Heces/microbiología , Humanos , Turquía
19.
Mikrobiyol Bul ; 19(2): 65-72, 1985 Apr.
Artículo en Turco | MEDLINE | ID: mdl-3892248

RESUMEN

In pregnant women, the main reservoirs of group B streptococci are rectum and urethra. The mother's birth canal and the newborn infant easily contact the organisms from these sites. We studied 40 women and their newborn babies to determine the relation between the maternal carriage and the neonatal group B streptococcal colonization. Vaginal, urethral and rectal swabs obtained from all pregnant women during labor. Within a few minutes after birth and on day 4 of life swab specimens were also taken from the external auditory canal, throat and umbilicus of the infants. The overall maternal carriage rate was found to be 10.0 percent. The frequency of transmission to the neonates was found to be 25.0 percent among maternal carriers. In early neonatal period, the colonization rate of group B streptococci was found to be 2,5 percent among the newborn population.


Asunto(s)
Portador Sano/microbiología , Recto/microbiología , Infecciones Estreptocócicas/microbiología , Streptococcus agalactiae/aislamiento & purificación , Uretra/microbiología , Vagina/microbiología , Adulto , Oído Externo/microbiología , Femenino , Humanos , Recién Nacido , Masculino , Faringe/microbiología , Embarazo , Ombligo/microbiología , Sistema Urinario/microbiología
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