RESUMEN
BACKGROUND: One of the biggest concerns in transfusion medicine is to avoid red blood cell alloimmunization. We evaluated the rate of alloimmunization in two groups of chronically transfused patients (A - not phenotyped and B - phenotyped for Rh/K antigens before the first transfusion) with primary haematological disorders and its distribution among the main haematological diseases, in order to adopt an efficient transfusional strategy. STUDY DESIGN AND METHODS: As methodology, we used the SIBAS and SAM databases for the retrospective study of all patients with primary haematological disorder between January 2011 and April 2013. RESULTS: A statistical difference in the rate of alloimmunization comparing groups A and B was found (P <0·0001). We also observed that alloimmunization was not homogeneously distributed in all primary haematological diseases. CONCLUSIONS: The Rh/K phenotype should be performed on all patients diagnosed with bone marrow failure, plasma cell dyscrasia and myelodysplastic syndrome in order to avoid alloimmunization. In patients with acute leukaemia and lymphoma it seems not necessary to perform it. In patients with haemoglobinopathy and myeloproliferative disorders, a larger group of patients is needed to decide its efficacy.
Asunto(s)
Bases de Datos Factuales , Transfusión de Eritrocitos/métodos , Enfermedades Hematológicas/sangre , Enfermedades Hematológicas/terapia , Sistema del Grupo Sanguíneo de Kell/sangre , Sistema del Grupo Sanguíneo Rh-Hr/sangre , Anciano , Anciano de 80 o más Años , Transfusión de Eritrocitos/efectos adversos , Femenino , Humanos , Isoantígenos/sangre , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de RiesgoRESUMEN
This study investigated whether a single intra-articular administration (IA) of dexamethasone (DEX) in horses at therapeutic dosage could exert a systemic effect by influencing the hypothalamic-pituitary-adrenal axis activity as a consequence of (limited) absorption and systemic distribution. The results indicated that DEX was detectable in urine collected 12-48 h after IA administration and that injection was accompanied by a reduced urine excretion of cortisol, 6ß-hydroxycortisol (6ßOHF) and two other metabolites of cortisol lasting up to 48 h post-DEX administration. The systemic effects in horses treated with DEX by IA route are similar to those that typically occur with short-term treatment including the reduction in urinary cortisol concentration.
Asunto(s)
Antiinflamatorios/farmacología , Dexametasona/farmacología , Caballos/sangre , Caballos/orina , Animales , Antiinflamatorios/administración & dosificación , Cromatografía Liquida , Dexametasona/administración & dosificación , Ensayo de Inmunoadsorción Enzimática/veterinaria , Hidrocortisona/análogos & derivados , Hidrocortisona/sangre , Hidrocortisona/orina , Inyecciones Intraarticulares , Masculino , Radioinmunoensayo/veterinaria , Espectrometría de Masas en TándemRESUMEN
The analysis of doping agents in biological fluids is of top significance in clinical and forensic toxicology. Herein we describe the study of a screening method for the detection of a mixture of drugs of potential abuse including cocaine and its metabolites. By using matrix-assisted laser desorption/ionization MALDI-TOF/TOF mass spectrometry. This screening procedure to detect the presence of different drugs, avoiding time consuming procedures could be useful in different fields of forensic analytical toxicology, including antidoping analysis.