RESUMEN
High power output and high conversion efficiency are crucial parameters for microbial fuel cells (MFCs). In our previous work, we worked with microfluidic MFCs to study fundamentals related to the power density of the MFCs, but nutrient consumption was limited to one side of the microchannel (the electrode layer) due to diffusion limitations. In this work, long-term experiments were conducted on a new four-electrode microfluidic MFC design, which grew Geobacter sulfurreducens biofilms on upward- and downward-facing electrodes in the microchannel. To our knowledge, this is the first study comparing electroactive biofilm (EAB) growth experiencing the influence of opposing gravitational fields. It was discovered that inoculation and growth of the EAB did not proceed as fast at the downward-facing anode, which we hypothesize to be due to gravity effects that negatively impacted bacterial settling on that surface. Rotating the device during the growth phase resulted in uniform and strong outputs from both sides, yielding individual power densities of 4.03 and 4.13 W m-2, which increased to nearly double when the top- and bottom-side electrodes were operated in parallel as a single four-electrode MFC. Similarly, acetate consumption could be doubled with the four electrodes operated in parallel.
RESUMEN
The main hurdle in leveraging microfluidic advantages in membraneless MFCs is their low electrode area-normalized power. For nearly a decade, maximum power densities have remained stagnant, while at the same time macrosystems continue to gather pace. To bridge this growing gap, we showcase a strategy that focuses on (i) technology improvements, (ii) establishment of record areal power densities, and (iii) presentation of different normalization methods that complement areal power densities and enable direct comparisons across all MFC scales. Using a pure-culture Geobacter sulfurreducens electroactive biofilm (EAB) in a new membraneless MFC that adheres to the strategy above, we observed optimal anode colonization, resulting in the highest recorded electrode areal power density for a microfluidic MFC of 3.88 W m-2 (24.37 kW m-3). We also consider new power normalization methods that may be more appropriate for comparison to other works. Normalized by the wetted cross-section area between electrodes accounts for constraints in electrode/electrolyte contact, resulting in power densities as high as 8.08 W m-2. Alternatively, we present a method to normalize by the flow rate to account for acetate supply, obtaining normalized energy recovery values of 0.025 kW h m-3. With these results, the performance gap between micro- and macroscale MFCs is closed, and a road map to move forward is presented.