RESUMEN

Comparative analysis of amino acid and nucleotides sequences of ORFs located in extended segments of the terminal variable regions in variola virus genome detected a promising locus for viral genotyping according to the geographic origin. This is ORF O1L of VARV. The primers were calculated for synthesis of this ORF fragment by PCR, which makes it possible to distinguish South America-Western Africa genotype from other VARV strains. Subsequent RFLP analysis reliably differentiated Asian strains from African strains (except Western Africa isolates). This method has been tested using 16 VARV strains from various geographic regions. The developed approach is simple, fast and reliable.

Asunto(s)

Reacción en Cadena de la Polimerasa/métodos , Viruela/virología , Virus de la Viruela/clasificación , Virus de la Viruela/aislamiento & purificación , África , Secuencia de Aminoácidos , Repetición de Anquirina/genética , Asia , Cartilla de ADN/genética , Variación Genética , Genoma Viral/genética , Humanos , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Polimorfismo de Longitud del Fragmento de Restricción , Sensibilidad y Especificidad , Alineación de Secuencia , América del Sur , Virus de la Viruela/genética