RESUMEN
It has been shown that biotopes of upper respiratory system and intestine were contaminated with yeast in 44.6% of patients with leukaemia (of 112 examined ones). Their quantity exceeds the boundary value for practically healthy people and is > or = 10(2) KOE/ml in the nasal activity and fauces and < or = 10(4) KOE/g in the intestine. It was established that in patients with leucemia the mycotic complications are mainly caused by anamorphous yeast of ascomycetic affinity. Candida albicans, as well as C. glabrata, C. rugosa and Candida sp. play the leading role. The Candida genus species are mainly sensitive to amphotericine B, clotrisamol and nistatin.
Asunto(s)
Intestinos/microbiología , Leucemia/microbiología , Micosis/prevención & control , Infecciones Oportunistas/prevención & control , Sistema Respiratorio/microbiología , Levaduras/aislamiento & purificación , Adulto , Anciano , Antifúngicos/farmacología , Humanos , Leucemia/complicaciones , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Micosis/complicaciones , Micosis/microbiología , Infecciones Oportunistas/complicaciones , Infecciones Oportunistas/microbiología , Levaduras/efectos de los fármacos , Adulto JovenRESUMEN
A taxonomic state of the collection strains of three marketable species of genus Debaryomyses has been specified. Sources of their isolation have been analyzed and a list of econiches where the yeast occur has been supplemented. The properties have been found which are recommended to be used as additional for differentiation of D. hansenii, D. polymorphus, D. pseudopolymorphus species (that was the ability to assimilate nitrites, growth in the medium with 24 % of NaCl, synthesis of riboflavin, availability of jelatinase activity).
Asunto(s)
Saccharomycetales , Clasificación , Saccharomycetales/clasificación , Saccharomycetales/crecimiento & desarrollo , Saccharomycetales/fisiología , Especificidad de la EspecieRESUMEN
Morphological and physiological properties of 52 strains of yeast Debaryomyces hansenii from the Ukrainian collection of microorganisms has been investigated. The belonging of 40 strains to the given species is confirmed. According to new classification 35 of them are referred to D. hansenii var. hansenii, five strains--to D.hansenii var. fabryi. It is shown, that assimilation of hydrocarbons and antagonistic properties are inherent in a small amount of strains. One half of the investigated debaryomycetes are riboflavin producers. The osmo- and galofility is inherent in all the investigated strains. These characteristics are proposed to be applied as additional ones in identification of these yeast.
Asunto(s)
Saccharomycetales/clasificación , Saccharomycetales/fisiología , Técnicas de Tipificación Micológica , Ósmosis , Filogenia , Riboflavina/biosíntesis , Saccharomycetales/metabolismo , UcraniaRESUMEN
Strains (103) of yeast from Pichia genus: P. anomala, P. guilliermondii and P. membranaefaciens species have been investigated. The researchers have found 87 strains (84.5%) capable to form extracellular and surface lectins with activity to 512 hemagglutinating units. The overwhelming number of strains (75.7-92.6%) synthesized lectins of the both forms. Considerably less quantity of lectin producers of some form--extracellular or surface ones--have been found. Availability of producers of extracellular lectins was characteristic to greater extent of the yeast of P. anomala and P. guilliermondii, and producers of surface lectins--mainly of P. guilliermondii. The most active producers of lectins have been isolated from human and animal organisms, soils and wine.
Asunto(s)
Lectinas/metabolismo , Pichia/aislamiento & purificación , Pichia/metabolismo , Pruebas de Hemaglutinación , Lectinas/análisis , Lectinas/biosíntesisRESUMEN
Hemagglutinating properties of the culture liquid and cells of 451 strains, 24 species, 7 genera of the museum and fresh yeast cultures have been investigated. The researchers have distinguished 333 strains (74%) capable for production of extracellular lectins and those bound to the cell surface with activity about 512-1024 hemagglutinating units. The extracellular lectins were found in 41% of lectin-synthesizing strains; the highest amount of their producers were found among representatives of genera Saccharomyces, Kluyveromyces, Cryptococcus. Strains of Debaryomyces genus mainly synthesized lectins of the bound form, and the yeast of Pichia genus--lectins of the both types. Affinity of the extracellular lectin Saccharomyces cerevisiae UCM Y-530 for D-gluconic acid has been established.
Asunto(s)
Lectinas/metabolismo , Levaduras/metabolismo , Candida/aislamiento & purificación , Candida/metabolismo , Cryptococcus/aislamiento & purificación , Cryptococcus/metabolismo , Medios de Cultivo , Pruebas de Hemaglutinación , Kluyveromyces/aislamiento & purificación , Kluyveromyces/metabolismo , Lectinas/análisis , Lectinas/biosíntesis , Pichia/aislamiento & purificación , Pichia/metabolismo , Rhodotorula/aislamiento & purificación , Rhodotorula/metabolismo , Saccharomyces cerevisiae/aislamiento & purificación , Saccharomyces cerevisiae/metabolismo , Especificidad de la Especie , Levaduras/aislamiento & purificaciónRESUMEN
Hemagglutinating properties of culture liquid and cells of 142 yeast strains of species Saccharomyces cerevisiae, Kluyveromyces marxianus, K. dobzhanskii, K. lactis var. lactis and K. lactis var. drosophilarum, isolated from various sources have been studied. A capacity of yeast (44% of the studied strains) to synthesize both lectins bound to a cell and extracellular lectins has been established; the latter have been found in yeast for the first time.