RESUMEN

Carnitine Palmitoyl Transferase Type II (CPT II) deficiency is a disorder of fatty acid beta oxidation that causes decreased adenosine triphosphate (ATP) and ketone production during periods of fasting or high energy requirements. Labor and delivery can precipitate attacks for parturients with this disorder, causing hypoglycemia, muscle weakness, rhabdomyolysis, and kidney failure. Anesthetic management considers the delivery mode and anesthetic medications available to reduce these risks. We present the case of a pregnant patient with CPT II deficiency with labor epidural analgesia and a vaginal delivery, with alternative plans had a different delivery mode been required.

RESUMEN

After tooth extraction, alveolar bone resorption and labial bone plate thinning occur due to the lack of periodontal ligaments. The socket shield method was developed to preserve the alveolar ridge. A split-mouth study was performed in which eight patients were treated using alloplast with socket shield on one side (alloplast group, control) and autogenous dentin graft with socket shield on the contralateral side (dentin group, test). After 3 months, a trephine bone core was collected from all sites and evaluated by histological, histomorphometric, and radiographic analysis. Thin bony trabeculae were formed surrounding the residual alloplast, while thicker trabeculae of bone formed and fused to the autogenous dentin. The percentage of newly formed bone was significantly higher in the dentin group when compared to the alloplast group (P = 0.020). Radiographically, there was no significant difference in the mean percentage increase in bone density from preoperative to post-grafting between the two groups. Moreover, when comparing the change in labial bone level from preoperative to 3 months post-grafting between the two groups, there was no significant difference. The autogenous dentin graft combined with socket shield could be a promising technique for socket preservation.

Asunto(s)

Pérdida de Hueso Alveolar , Aumento de la Cresta Alveolar , Implantes Dentales , Humanos , Alveolo Dental/cirugía , Ligamento Periodontal/cirugía , Aumento de la Cresta Alveolar/métodos , Extracción Dental , Pérdida de Hueso Alveolar/diagnóstico por imagen , Pérdida de Hueso Alveolar/cirugía , Dentina/cirugía

RESUMEN

STATEMENT OF PROBLEM: Zirconia has been widely used in restorative dentistry because of its favorable strength and esthetics. However, its polycrystalline structure presents a challenge for resin bonding. Significant research into surface treatments of zirconia to improve bonding has been reported, yet a universally accepted protocol remains elusive. PURPOSE: The purpose of this systematic review and meta-analysis was to evaluate the performance of anterior single-retainer zirconia resin-bonded fixed dental prostheses (RBFDPs) and review the bonding protocols used in the published data. MATERIAL AND METHODS: An electronic search of English language literature was conducted in the PubMed and Ovid (MEDLINE) databases. Following the application of inclusion and exclusion criteria, the research was evaluated to assess the quantitative data. RESULTS: Of the included studies, 1 randomized controlled trial, 3 prospective cohort studies, and 2 retrospective cohort studies reporting on anterior single-retainer zirconia RBFDPs were evaluated. Over a 3- to 10-year period, the success rate of anterior single-retainer zirconia RBFDPs was over 80%. Although there were reports of debonds, this is a minor complication as rebonding is usually possible. Furthermore, catastrophic fractures of the entire prosthesis were rare. CONCLUSIONS: Evidence for the use of single-retainer zirconia RBFDPs as a suitable treatment option for the replacement of anterior missing teeth is considerable. Based on the findings of this systematic review and meta-analysis, a 2-step process comprising micromechanical retention using airborne-particle abrasion and subsequent resin-zirconia adhesion by the application of 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) can be recommended.

RESUMEN

Branchial arches are embryologic structures that develop between the fourth and seventh gestational week. Anomalies may form if these structures fail to develop. The majority of cases are diagnosed during childhood, with surgical excision recommended to prevent risk of infection, growth or malignancy. We report an unusual case of a 72-year-old man with severe cardiac comorbidities who presented with a large second branchial arch cyst extending into the oropharynx. General anaesthesia to facilitate surgical excision was deemed too risky. Therefore, we performed successful ultrasound-guided superficial and deep cervical plexus blocks as a sole mode of anaesthesia. This case highlights how regional anaesthesia can be utilised to facilitate surgery in high-risk patients, as well as presenting an alternative for general anaesthesia.

RESUMEN

This paper describes the construction of equilibrium configurations for smectic-A liquid crystals subjected to nonuniform physical boundary conditions, with two-dimensional dependence on the director and layer normal, and a nonlinear layer function. Euler-Lagrange equations are constructed that describe key properties of liquid crystals confined between two boundaries exhibiting spatial imperfections. The results of the model are shown to be consistent with previous published findings in simple domains while results are obtained on how the structure of the liquid crystals changes in response to boundary perturbations. Domain sizes are considered representing those currently used in applications while predictions in smaller domains at the limit of current technologies are also made. In particular, it is shown that the curvature along a boundary impacts on the liquid crystal's structure distant from the boundary feature and therefore previously developed mathematical models, that essentially reduced the problem to a single spatial dimension, cannot be used in such circumstances. Consequences for practical applications are briefly discussed.

RESUMEN

Although plastic induces environmental damages, almost the consumption of poly(vinyl chloride) never stops increasing. Therefore, this work abstracted by two parts, first, synthesis of Schiff bases 1-4 compounds through the reaction of amino group with appropriate aromatic aldehyde, reaction of PVC with Schiff bases compounds 1-4 in THF to form a new modified PVC-1, PVC-2, PVC-3, and PVC-4. The structures of Schiff bases 1-4 and the modified PVC-1, PVC-2, PVC-3, and PVC-4 have been characterized by different spectroscopic analyses. Second, the influence of introducing 4-amino-1,2,4-triazole as a pendent groups into PVC chain investigated on photostability rules of tests. The modified polymers photostability investigated by observing indices (ICO, Ipo, and IOH), weight loss, UV and morphological studies, and all results obtained indicated that PVC-1, PVC-2, PVC-3 and PVC-4 gave lower growth rate of ICO, IPO, and IOH through UV exposure time. The photostability are given as PVC-4 < PVC-3 < PVC-2 < PVC-1 from different mechanisms which suggested building on existence of 4-amino-1,2,4-triazole moieties in the polymer chain.

Asunto(s)

Polímeros/efectos de la radiación , Cloruro de Polivinilo/efectos de la radiación , Rayos Ultravioleta , Modelos Químicos , Polímeros/química , Cloruro de Polivinilo/química , Exposición a la Radiación

RESUMEN

OBJECTIVES: Injectable urethral bulking agents are commonly used to manage stress urinary incontinence. Urologic or other symptoms may prompt pelvic imaging at a later date, when bulking agents may be visualized and incorrectly interpreted. Our goal was to evaluate the incidence of misdiagnosis and which pathologies were the most common misinterpretations and their frequency. METHODS: All records were reviewed for patients who underwent periurethral injection for stress urinary incontinence for pelvic imaging after treatment from 2005 to 2015. Radiological reports were reviewed for any description potentially related to injection therapy, and descriptive statistics performed. RESULTS: A total of 528 patients underwent injection of a urethral bulking agent. Of these, 79 patients (15%) had a total of 111 additional abdominal or pelvic imaging studies performed with abnormal periurethral findings mentioned. Thirty-nine (35%) of 111 studies were correctly interpreted as urethral bulking agents, and in 72 (65%) of 111 studies, the urethral bulking agents were not correctly identified. The most common misdiagnoses were bladder calcification (26; 23%), urethral diverticulum with stone (12; 11%), periurethral calcification (9; 8%), unknown pelvic density (8; 7%), and mass suspicious for malignancy (6; 5%). CONCLUSIONS: Urethral bulking agents commonly were not mentioned on subsequent imaging but, when commented on, were misinterpreted 65% of the time including worrisome pathologies (diverticulum with stone, unknown mass, and malignancy), requiring subsequent evaluation and potentially procedural/surgical management. It is critical for the ordering clinician to inform the radiologist of this history and for radiologists to consider bulking agents in the differential diagnosis of radiographic findings in this location.

Asunto(s)

Materiales Biocompatibles/administración & dosificación , Errores Diagnósticos/estadística & datos numéricos , Enfermedades Uretrales/diagnóstico por imagen , Incontinencia Urinaria de Esfuerzo/terapia , Adulto , Anciano , Anciano de 80 o más Años , Materiales Biocompatibles/efectos adversos , Femenino , Humanos , Inyecciones , Imagen por Resonancia Magnética , Masculino , Registros Médicos , Persona de Mediana Edad , Estudios Retrospectivos , Tomografía Computarizada por Rayos X , Ultrasonografía , Uretra/diagnóstico por imagen , Vejiga Urinaria/diagnóstico por imagen , Adulto Joven

RESUMEN

The fabrication of tunable poly(vinyl chloride) porous films containing polyphosphate as an additive was successful. Irradiation of poly(vinyl chloride) films containing polyphosphate at a low concentration (0.5% by weight) with an ultraviolet light (λmax = 313 nm) for 300 h leads to the formation of a honeycomb like structure. The scanning electron microscopy images, at different magnification power, confirmed the production of the PVC honeycomb-like structure. The morphological images of the polymeric film showed a rough surface and a large number of regularly distributed hexagonal pores. The number of pores increased upon irradiation time and it was maximum after 300 h. The honeycomb structure formation could be due to the regular aggregation of polyphosphate among the polymeric chains, the increase in solution intrinsic viscosity and evaluation of hydrogen chloride gas through dehydrochlorination process.

RESUMEN

Polystyrene films containing a low concentration of three highly aromatic Schiff bases were prepared using the casting method. The polystyrene films were irradiated with ultraviolet light (300 h). The polystyrene infrared spectra, weight loss, molecular weight reduction and the surface morphology were examined upon irradiation. The Schiff bases acted as photostabilizers and reduced the photodegradation of polystyrene films to a significant level in comparison to the blank film. The images recorded of the surface of the miscible polystyrene/Schiff base blends showed novel ball-like microspheres with a diameter of 3.4⁻4.3 µm. The Schiff bases were able to endow excellent protection to polystyrene against ultraviolet irradiation.

RESUMEN

Three polyphosphates were used as inhibitors for poly(vinyl chloride) (PVC) photodegradation. The polyphosphates were added to PVC at a concentration of 0.5% by weight. The PVC films (40 µm thickness) were irradiated at room temperature with ultraviolet (UV) light for up to 300 h. The changes in PVC films after irradiation were monitored by Fourier transform infrared spectroscopy, weight loss, viscosity-average molecular weight determination, and atomic force microscopy. These changes were very noticeable in the blank PVC films compared to the ones obtained when additives were used. The polyphosphates can inhibit the PVC photodegradation through direct absorption of UV light, interactions with PVC chains, and acting as radical scavengers.

Asunto(s)

Fotólisis/efectos de los fármacos , Polifosfatos/farmacología , Cloruro de Polivinilo/química , Microscopía de Fuerza Atómica , Estructura Molecular , Peso Molecular , Espectroscopía Infrarroja por Transformada de Fourier , Viscosidad

RESUMEN

Three new tetra-Schiff bases were synthesized and characterized to be used as photostabilizers for poly(vinyl chloride) (PVC) films. The photostability of PVC films (40 µm thickness) in the presence of Schiff bases (0.5 wt %) upon irradiation (300 h) with a UV light (λmax = 365 nm and light intensity = 6.43 × 10-9 ein∙dm-3∙s-1) was examined using various spectroscopic measurements and surface morphology analysis. The changes in various functional groups' indices, weight and viscosity average molecular weight of PVC films were monitored against irradiation time. The additives used showed photostability for PVC films, with Schiff base 1 being the most effective additive upon irradiation, followed by 2 and 3. The atomic force microscopy (AFM) images for the PVC surface containing Schiff base 1 after irradiation were found to be smooth, with a roughness factor (Rq) of 36.8, compared to 132.2 for the PVC (blank). Several possible mechanisms that explain PVC photostabilization upon irradiation in the presence of tetra-Schiff bases were proposed.

Asunto(s)

Membranas Artificiales , Cloruro de Polivinilo/química , Bases de Schiff/síntesis química , Microscopía de Fuerza Atómica , Peso Molecular , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie , Rayos Ultravioleta

RESUMEN

Three phosphate esters 1⁻3 were successfully synthesized from the reaction of 2-, 3- and 4-hydroxybenzaldehyde with phosphoryl chloride. Reactions of 1⁻3 with benzidine in the presence of glacial acetic acid gave the corresponding novel phosphorus organic polymers 4⁻6 containing the azomethane linkage. The structures of the synthesized compounds were confirmed by Fourier transform infrared spectroscopy, nuclear magnetic resonance and elemental analysis. Interesting physiochemical properties for the polymeric materials 4⁻6 were observed using a combination of several techniques such as gel permeation chromatography, scanning electron microscopy, Brunauer⁻Emmett⁻Teller and nitrogen adsorption⁻desorption isotherm, Barrett⁻Joyner⁻Halenda and H-sorb 2600 analyzer. The mesoporous polymers 4⁻6 exhibit tunable porosity with Brunauer⁻Emmett⁻Teller surface area (SABET = 24.8⁻30 m²·g⁻1), pore volume (0.03⁻0.05 cm³·g⁻1) and narrow pore size distribution, in which the average pore size was 2.4⁻2.8 nm. Polymers 4⁻6 were found to have high gas storage capacity and physico-chemical stability, particularly at a high pressure. At 323 K and 50 bars, polymers 4⁻6 have remarkable carbon dioxide uptake (up to 82.1 cm³·g⁻1) and a low hydrogen uptake (up to 7.4 cm³·g⁻1). The adsorption capacity of gasses for polymer 5 was found to be higher than those for polymers 4 and 6.

RESUMEN

In the crystal structure of the title compound, 2C(10)H(7)NO(4)·C(14)H(13)N(3)O(2), the two independent acid mol-ecules are connected through strong O-H⋯N and O-H⋯O hydrogen bonds to the central mol-ecule of the anti-tubercular drug N'-[(E)-4-meth-oxy-benzyl-idene]pyridine-4-carbohydrazide. Two such trimolecular units related by an inversion centre inter-act through a pair of N-H⋯O hydrogen bonds, forming a 3 + 3 mol-ecular aggregate. The dihedral angle between the aromatic rings of the hydrazone mol-ecule is 1.99 (12)°. The crystal packing features weak C-H⋯O and π-π stacking inter-actions, with centroid-centroid distances of 3.8460 (19) and 3.8703 (13) Å.

RESUMEN

Long-term potentiation in sympathetic ganglia (gLTP) is an activity-dependent unique form of synaptic plasticity in that it is serotonin-dependent and can be completely inhibited by 5-HT3 receptor antagonists. Long lasting enhancement of the basal tone of ganglionic transmission seen with gLTP results in a sustained increase in peripheral resistance that leads to elevated blood pressure. We examined the possibility that, in sympathetic ganglia, stress-induced gLTP may be responsible for the expression of stress hypertension. Chronic treatment of male and female Wistar rats with a 5-HT3 receptor antagonist, tropisetron (ICS; 5 mg/kg/day) or ondansetron (0.5 mg/kg/day), prevented or reversed psychosocial stress-induced increases in blood pressure in stressed rats with no significant effect on blood pressure of unstressed control rats. Pharmacological and electrophysiological evidence that supports the presence of gLTP in ganglia isolated from stressed hypertensive rats includes inhibition of basal synaptic transmission by 5-HT3 antagonists, failure to induce gLTP with repetitive stimulation indicating occlusion of gLTP due to saturation and a left hand shift of the input/output curve. We suggest that a sustained stress-induced increase in central sympathetic efferent impulses to ganglia may provide the repeated high frequency presynaptic activity required to induce gLTP in sympathetic ganglia, thereby enhancing sympathetic tone to blood vessels resulting in hypertension.

Asunto(s)

Enfermedades del Sistema Nervioso Autónomo/psicología , Ganglios Simpáticos/fisiopatología , Hipertensión/psicología , Potenciación a Largo Plazo/fisiología , Estrés Psicológico/complicaciones , Animales , Enfermedades del Sistema Nervioso Autónomo/etiología , Enfermedades del Sistema Nervioso Autónomo/fisiopatología , Vasos Sanguíneos/inervación , Vasos Sanguíneos/fisiopatología , Modelos Animales de Enfermedad , Femenino , Ganglios Simpáticos/efectos de los fármacos , Ganglios Simpáticos/metabolismo , Hipertensión/etiología , Hipertensión/fisiopatología , Potenciación a Largo Plazo/efectos de los fármacos , Masculino , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Técnicas de Cultivo de Órganos , Ratas , Ratas Wistar , Receptores de Serotonina 5-HT3/metabolismo , Serotonina/metabolismo , Antagonistas del Receptor de Serotonina 5-HT3 , Antagonistas de la Serotonina/farmacología , Estrés Psicológico/fisiopatología , Transmisión Sináptica/efectos de los fármacos , Transmisión Sináptica/fisiología , Vasoconstricción/fisiología

RESUMEN

We present a case of abrupt hemodynamic and mental status changes that occurred during shoulder surgery. During interscalene anesthesia for rotator cuff repair, there was abrupt onset of altered mental status and hemodynamic changes, which had a variety of possible contributing causes. Complete recovery occurred during care in the post-anesthesia care unit. A variety of physiologic changes can occurred during interscalene anaesthesia for shoulder surgery, which require prompt identification and management.

Asunto(s)

Plexo Braquial , Complicaciones Intraoperatorias/etiología , Bloqueo Nervioso/efectos adversos , Hombro/cirugía , Femenino , Hemodinámica , Humanos , Persona de Mediana Edad

Asunto(s)

Anafilaxia/etiología , Hipersensibilidad al Látex/patología , Adulto , Cesárea , Femenino , Sufrimiento Fetal/etiología , Humanos , Exposición por Inhalación , Embarazo

RESUMEN

Endochondral ossification (EO) occurs in the growth plate where chondrocytes pass through discrete stages of proliferation, maturation, hypertrophy, and calcification. We have developed and characterized a novel bovine cell culture model of EO that mirrors these events and will facilitate in vitro studies on factors controlling chondrocyte differentiation. Chondrocytes derived from the epiphyses of long bones of fetal calves were treated with 5-azacytidine (aza-C) for 48 h. Cultures were maintained subsequently without aza-C and harvested at selected time points for analyses of growth and differentiation status. A chondrocytic phenotype associated with an extensive extracellular matrix rich in proteoglycans and collagen types II and VI was observed in aza-C-treated and -untreated cultures. aza-C-treated cultures were characterized by studying the expression of several markers of chondrocyte differentiation. Parathyroid hormone-related protein (PTHrP) and its receptor, both markers of maturation, were expressed at days 5-9. Type X collagen, which is restricted to the stage of hypertrophy, was expressed from day 11 onward. Hypertrophy was confirmed by a 14-fold increase in cell size by day 15 and an increased synthesis of alkaline phosphatase during the hypertrophic period (days 14-28). The addition of PTHrP to aza-C-treated cultures at day 14 led to the down-regulation of type X collagen by 6-fold, showing type X collagen expression is under the control of PTHrP as in vivo. These findings show that aza-C can induce fetal bovine epiphyseal chondrocytes to differentiate in culture in a manner consistent with that which occurs during the EO process in vivo.

Asunto(s)

Calcificación Fisiológica , Condrocitos/citología , Modelos Biológicos , Animales , Azacitidina/farmacología , Secuencia de Bases , Huesos/embriología , Bovinos , Células Cultivadas , Condrocitos/efectos de los fármacos , Cartilla de ADN , Inmunohistoquímica , Proteína Relacionada con la Hormona Paratiroidea , Fenotipo , Proteínas/farmacología

RESUMEN

There are two forms of procollagen type II (IIA and IIB), both of which are expressed during chondrogenesis. Procollagen type IIA also is present at sites of developmental epithelial-mesenchymal interaction. Malignant transformation is associated with disturbed epithelial-mesenchymal interaction and the reappearance of fetal characteristics. This study aims to determine whether or not procollagen type IIA is re-expressed in oral squamous cell carcinoma (OSCC). Immunoperoxidase techniques were applied to frozen and paraffin sections of OSCC (n = 30) and normal oral mucosa (n = 5). In the carcinoma group, strong cytoplasmic staining for collagen type II was present (25/30). Staining was weak or absent in the stroma, and absent from the normal oral mucosa. Frozen sections from 10 of the carcinoma cases which showed positive staining were incubated with antibodies specific for procollagen type IIA and visualised using immunofluorescence. Staining was evident in each case and was particularly strong in the region of the basement membrane. Slot-blot analysis of collagen extracts from OSCC supported the immunohistochemical findings. We conclude, therefore, that procollagen type IIA is re-expressed in OSCC.

Asunto(s)

Carcinoma de Células Escamosas/metabolismo , Neoplasias de la Boca/metabolismo , Proteínas de Neoplasias/metabolismo , Procolágeno/metabolismo , Humanos , Técnicas para Inmunoenzimas

RESUMEN

Type VI collagen appears central to the maintenance of tissue integrity. In adult articular cartilage, type VI collagen is preferentially localised in the chondron where it may be involved in cell attachment. In actively remodelling developing cartilage, the distribution is less certain. We have used confocal immunohistochemistry and in situ hybridisation to investigate type VI collagen distribution in third trimester bovine proximal femoral epiphyses. In general, type VI collagen immunofluorescence was concentrated in the chondrocyte pericellular matrix, with staining intensity strongest in regions which persist to maturity and weakest in regions that remodel during development. Type VI collagen was also present in cartilage canals. In the growth plate and around the secondary centre of ossification, the intensity of type VI collagen stain rapidly decreased with chondrocyte maturation and was absent at hypertrophy, except where canal branches penetrated the growth plate and stain was retained around the adjacent chondrocytes. In situ hybridisation confirmed the presence of type VI collagen mRNA in cartilage canal mesenchymal cells but the signal was low in chondrocytes, suggesting minimal levels of synthesis and turnover. The results are consistent with a role for type VI collagen in stabilising the extracellular matrix during development.

Asunto(s)

Colágeno/metabolismo , Cabeza Femoral/embriología , Animales , Cartílago Articular/embriología , Cartílago Articular/metabolismo , Bovinos , Colágeno/genética , Cabeza Femoral/metabolismo , Placa de Crecimiento/embriología , Placa de Crecimiento/metabolismo , Humanos , Técnicas para Inmunoenzimas , Hibridación in Situ , Conejos

RESUMEN

PURPOSE: In a previous investigation into corneal autoimmunity, it was demonstrated that a putative autoantigen, a protein of 66 kDa, present in bovine corneal epithelium, binds circulating autoantibodies in approximately 60% of patients with Wegener's granulomatosis (WG). The aim of the present study was to characterize and identify the 66-kDa protein. METHODS: A purification protocol was established for the 66-kDa protein using standard chromatography techniques. During the purification procedure it became clear that the 66-kDa protein detected in patients' sera was in fact two proteins, both running at 66 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, that eluted in different fractions on DE-52 chromatography columns. These two proteins have been labeled bovine corneal epithelial antigen-A and -B (BCEA-A and BCEA-B). Further investigations of antibody binding have demonstrated that patients' sera bind to either one or the other of these proteins with no cross-reactivity between them. Separated BCEA-A and BCEA-B protein extracts were immunoblotted with 27 WG patients' sera, 10 Churg-Strauss syndrome (CSS) patients' sera, 31 rheumatoid arthritis (RA) patients' sera, and 40 healthy control subjects' sera from the blood bank. RESULTS: Forty-six percent of WG patients' sera had antibodies to one of the 66-kDa antigens, whereas none of the healthy control subjects' sera had 66-kDa antibodies (P < 10(-5)). In the WG group, 31% were positive to BCEA-A (versus controls, P = 0.0023), and 15% were positive to BCEA-B. WG patients with peripheral ulcerative keratitis (PUK) had a significant association with anti-BCEA-A antibodies when compared with healthy control subjects (50%, P < 10(-6)). However, in the RA group with no eye disease there was an association with BCEA-A (25%, P = 0.011) but not in the RA group with PUK. The frequency of anti-BCEA-B antibodies was significantly increased in patients with CSS (60%, P < 10(-7)). CONCLUSIONS: In summary, it has been shown that vasculitis patients have antibodies to two 66-kDa corneal antigens and that autoantibodies to these antigens are mutually exclusive. It has also been shown that antibodies to BCEA-B are associated with CSS, whereas BCEA-A antibodies are associated with WG and RA.

Asunto(s)

Autoanticuerpos/análisis , Autoantígenos/metabolismo , Conjuntivitis/metabolismo , Úlcera de la Córnea/metabolismo , Epitelio Corneal/metabolismo , Vasculitis/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Animales , Artritis Reumatoide/inmunología , Artritis Reumatoide/metabolismo , Artritis Reumatoide/patología , Autoantígenos/inmunología , Autoantígenos/aislamiento & purificación , Bovinos , Conjuntivitis/inmunología , Conjuntivitis/patología , Úlcera de la Córnea/inmunología , Úlcera de la Córnea/patología , Reacciones Cruzadas , Electroforesis en Gel Bidimensional , Epitelio Corneal/química , Técnica del Anticuerpo Fluorescente Indirecta , Humanos , Immunoblotting , Persona de Mediana Edad , Peso Molecular , Conejos , Vasculitis/inmunología , Vasculitis/patología