RESUMEN
Xanthine oxidase activity: O2-dependent and NAD+-dependent forms, were carried out in cytosol supernatant of Rat liver homogenat with adjuvant and hepatocytes induced arthritis and hepatitis. Both forms were increased without modification of their ratio. These results suggest that xanthine oxidase was implicated in the inflammatory reaction.
Asunto(s)
Artritis Experimental/enzimología , Artritis/enzimología , Hepatitis/enzimología , Hígado/enzimología , Xantina Oxidasa/metabolismo , Aerobiosis , Animales , Citosol/enzimología , Modelos Animales de Enfermedad , Masculino , NAD/farmacología , Oxígeno/farmacología , Ratas , Ratas EndogámicasRESUMEN
Xanthine oxidase activity: NAD+-dependent form (D) and O2-dependent form (O) were carried out in cytosol supernatants of connective tissue growth (T.C.N.F.), skin tail, liver and plasma of carrageenan induced granuloma in the Rat. The specific activities of skin, liver and plasma were normal in animals with a granuloma. The total specific activity (D + O): 7.53 +/- 0.98 mU/mg protein, and the percentage of form O: 51.6 +/- 5.1 of the granulomatous tissue as compared to the tail are significantly increased. These results suggest the likely function of xanthine oxidase during the inflammatory response.
Asunto(s)
Granuloma/enzimología , NAD/metabolismo , Oxígeno/metabolismo , Xantina Oxidasa/metabolismo , Animales , Carragenina , Granuloma/inducido químicamente , Masculino , Ratas , Ratas EndogámicasAsunto(s)
Dieta Reductora , Obesidad/metabolismo , Errores Innatos del Metabolismo de la Purina-Pirimidina/metabolismo , Purinas/metabolismo , Xantinas/orina , Peso Corporal , Femenino , Humanos , Masculino , Persona de Mediana Edad , Linaje , Errores Innatos del Metabolismo de la Purina-Pirimidina/genéticaAsunto(s)
Gota/genética , Hipoxantina Fosforribosiltransferasa/deficiencia , Síndrome de Lesch-Nyhan/genética , Manifestaciones Neurológicas , Adolescente , Adulto , Niño , Gota/complicaciones , Gota/etiología , Humanos , Síndrome de Lesch-Nyhan/diagnóstico , Masculino , Persona de Mediana Edad , LinajeRESUMEN
The study with labelled compound has shown that in rat, as in man, thiopurinol is primarily and rapidly converted into oxithiopurinol. More than 80% of the radioactivity of plasma was identified as oxithiopurinol less than 10 minutes after intravenous or oral administration. The parameters of oxithiopurinol were estimated from the decline of radioactivity in blood from 5 minutes to 8 hours after intravenous administration of 4 microCi/100 g of thiopurinol (8 animals) or oxithiopurinol (5 animals). Half lives of oxithiopurinol t 1/2 (beta) were 2.52 +/- 0.78 hr and 2.90 +/- 1.00 hr and clearance 2.00 +/- 0.36 ml/min. and 3.38 +/- 0.69 respectively. Estimate of renal clearance for oxithiopurinol in rat was 4,17 ml/min. The rapidity of hydroxylation and of elimination of oxithiopurinol might be assumed to be the cause of the apparent ineffectiveness of thiopurinol in vivo on xanthine oxidase activity.
Asunto(s)
Oxipurinol/metabolismo , Pirimidinas/metabolismo , Animales , Cinética , Masculino , Oxipurinol/análogos & derivados , Ratas , Factores de TiempoAsunto(s)
Xantina Oxidasa/análisis , Animales , Citoplasma/enzimología , Histocitoquímica , Humanos , Hígado/citología , Hígado/enzimología , RatasRESUMEN
A chromatographic technique on anion exchange column was developed. It permets to determine unmetabolized pyrazinamide and three major metabolities: pyrazinoic acid, 5-hydroxypyrazinoic acid and an unidentified compound. In a xanthinuric patient only traces of 5-hydroxypyrazinoic acid were found 12 hours after 3 g pyrazinamide were given. These finding confirms that pyrazinoic acid is oxidised through the the action of xanthine oxidase. Both the clearances of hypoxanthine and of xanthine are as rapid as that of endogenous creatinine in a xanthinuric patient. But the effects of pyrazinamide are different on both purine bases. Urinary excretion of hypoxanthine is slightly but not significantly reduced while excretion of xanthine is decreased by about 75%. Evidence was demonstrated that pyrazinoic acid is likely the agent causing xanthine retention. These results suggest that the mechanisms of renal transport of xanthine and hypoxanthine are different.
Asunto(s)
Hipoxantinas/orina , Errores Innatos del Metabolismo de la Purina-Pirimidina/orina , Pirazinamida/farmacología , Xantinas/orina , Cromatografía por Intercambio Iónico/métodos , Depresión Química , Humanos , Riñón/efectos de los fármacos , Riñón/metabolismo , Pirazinamida/metabolismo , Pirazinamida/orina , Xantina Oxidasa/deficienciaRESUMEN
We have carried out biochemical and clinical studies on a large family in which xanthinuria, xanthine lithiasis, uric acid lithiasis and/or gout were discovered. The analysis of its pedigree has shown that : a) the mode of transmission of xanthinuria is autosomal recessive; b) the occurence of xanthine urolithiasis is likely to be due to the association of a second genetic disorder.
Asunto(s)
Gota/genética , Cálculos Urinarios/genética , Xantinas/orina , Femenino , Gota/complicaciones , Gota/orina , Humanos , Masculino , Linaje , Cálculos Urinarios/complicaciones , Cálculos Urinarios/orinaRESUMEN
New drugs are proposed in an attempt to reduce the overformation of uric acid in man. The procedure is based on the inhibition of human purine nucleoside phosphorylase selectively investigated in its phosphorolytic sequence. But for technical reasons, analogous inactivation of the enzyme in its comparative guanosine arsenolysis is subject to experimentation.
Asunto(s)
Eritrocitos/enzimología , Pentosiltransferasa/antagonistas & inhibidores , Purina-Nucleósido Fosforilasa/antagonistas & inhibidores , Pirimidinas/farmacología , Animales , Arseniatos/metabolismo , Guanosina/metabolismo , Humanos , Ratones , Oxipurinol/análogos & derivados , Purina-Nucleósido Fosforilasa/sangre , Ratas , Relación Estructura-Actividad , Xantina Oxidasa/antagonistas & inhibidoresAsunto(s)
Gota/genética , Cálculos Renales/genética , Xantinas/orina , Adolescente , Adulto , Factores de Edad , Anciano , Niño , Preescolar , Creatinina/orina , Femenino , Genes Recesivos , Gota/orina , Humanos , Masculino , Persona de Mediana Edad , Linaje , Ácido Úrico/sangre , Ácido Úrico/orinaAsunto(s)
Hígado/enzimología , Xantina Oxidasa/aislamiento & purificación , Animales , Histocitoquímica , Métodos , RatasRESUMEN
Oxithiopurinol, [4-thio-6-hydroxypyrazolo(3,4-d)pyrimidine] has been caracterized in urines of patients treated with Thiopurinol : the principle involved is the precipitation of both oxithiopurinol and uric acid in combination with cupric hydroxide. The urinary oxithopurinol content is determined by means of anion and cation exchange resins. 56 to 70 per cent of the daily dose of Thiopurinol are eliminated in urine as oxithiopurinol.