RESUMEN
Many blood-feeding arthropods use volatile organic compounds (VOCs) to detect their vertebrate hosts. The role of chemical interactions in mediating the behavior of hematophagous insects and ticks has been investigated before but remains poorly understood in hematophagous mesostigmatic mites. The poultry red mite Dermanyssus gallinae is an obligatory blood-sucking mesostigmatic mite that feeds on birds and causes damage in poultry farms. We characterized the attractive response of D. gallinae to candidate VOCs previously reported from the odor emitted by living hens. We performed in-vitro choice-test bioassays as well as semi-field and field trials using baited and unbaited traps, in the presence and absence of hens. Among different tested combinations of VOCs, a blend of 5 VOCs (mix1.0) was significantly attractive to our reference population of D. gallinae in vitro, whereas the same individual compounds tested alone were not attractive. Ammonia was attractive on its own and increased the mix1.0 attractiveness. The attractiveness of mix1.0 was confirmed at 'natural' spatial scales in the absence of hens both at the lab and on the farm that provided the reference population. The presence of hens inhibited the mix1.0 attractiveness. The attractive power of mix1.0 was not found in other farms. This research is an important step to advance our understanding of host-parasite interactions in hematophagous mesostigmatic mites and paves the way for developing alternative control tools against D. gallinae by interfering with chemical interactions. Moreover, it underlines the importance of assessing kairomonal activity on different pest populations when developing attract-and-kill systems.
Asunto(s)
Infestaciones por Ácaros , Ácaros , Enfermedades de las Aves de Corral , Compuestos Orgánicos Volátiles , Animales , Femenino , Aves de Corral/parasitología , Infestaciones por Ácaros/epidemiología , Infestaciones por Ácaros/parasitología , Infestaciones por Ácaros/veterinaria , Pollos/parasitología , Enfermedades de las Aves de Corral/epidemiología , Enfermedades de las Aves de Corral/parasitología , Ácaros/fisiología , Compuestos Orgánicos Volátiles/farmacologíaRESUMEN
The pollination of two oil palm species, Elaeis guineensis Jacquin and Elaeis oleifera Cortés (Arecales: Arecaceae), depends on a mutualistic relation with insects, which use male inflorescences as a brood site, and visits female inflorescences lured by the emitted odor, which is similar to that of males. Although the activity of visiting the inflorescences by these insects is critical for the adequate natural pollination of the host plant, their activity is poorly documented. In the present study, we determine the diel activity of two specialized pollinator weevils (Coleoptera: Curculionidae) on inflorescences of their respective host-palm: Elaeidobius kamerunicus Faust specialized on E. guineensis, and Grasidius hybridus O'Brien and Beserra specialized on E. oleifera. The average timing of activity was studied by using passive interception traps. Then the pattern and the duration were refined by using aspiration trapping within the active period for each insect species at the male and female inflorescences. All the experiments were conducted in an Ecuadorian oil palm plantation, located close to Amazonian forest. El. kamerunicus and G. hybridus were found to be the pollinators of E. guineensis and E. oleifera, respectively. The two species differed in their diel pattern of activity: E. kamerunicus was active in the morning and G. hybridus during a short period at dusk. For both palm species, insect visits were synchronous on both male and female inflorescences. The synchronicity is discussed as a strategy to maintain the relation mutualistic between partners. These findings increase our understanding of the oil palm pollination system.