RESUMEN

We aimed at establishing hybridoma cells secreting monoclonal antibodies (mAbs) against E1 synthetic peptide of HCV. BALB/c mice were immunized with HCV E1-synthetic peptide (GHRMAWDMM) and its spleenocytes were fused with the P3NS1 myeloma cell line. Two highly reactive and specific mAbs (10C7 IgG2b mAb, and 10B2 IgG1 mAb) were generated. The target HCV E1 antigen was identified at approximately 38 kDa in serum of infected individuals. A newly developed ELISA detected the target antigen in 90% of sera from HCV RNA infected individuals with a specificity of 84%. So, the generated mAbs may provide promising probes for serodiagnosis of HCV infection.

Asunto(s)

Anticuerpos Monoclonales/inmunología , Hepatitis C/diagnóstico , Hibridomas , Proteínas del Envoltorio Viral/sangre , Proteínas del Envoltorio Viral/inmunología , Adolescente , Adulto , Animales , Anticuerpos Monoclonales/biosíntesis , Anticuerpos Monoclonales/aislamiento & purificación , Western Blotting , Línea Celular , Ensayo de Inmunoadsorción Enzimática , Femenino , Antígenos de la Hepatitis C/sangre , Antígenos de la Hepatitis C/química , Antígenos de la Hepatitis C/inmunología , Humanos , Masculino , Ratones , Ratones Endogámicos BALB C , Persona de Mediana Edad , Péptidos/síntesis química , Péptidos/química , Péptidos/inmunología , Pruebas Serológicas , Proteínas del Envoltorio Viral/química