RESUMEN
OBJECTIVE: To investigate the effect of Euro-Collins and Belzer solutions in a sequential preservation of the pancreas. METHODS: Forty-five Wistar-EPM rats were divided into four groups, according to the solution used during preservation: (1) saline solution (SF): animals perfused and preserved with saline solution; (2) Euro-Collins group (C): animals perfused and preserved with Euro-Collins solution; (3) Belzer group (B): animals perfused and preserved with Belzer solution; (4) Euro-Collins/Belzer group (CB): animals perfused with equal parts of Euro-Collins and Belzer solutions sequentially and preserved with Belzer solution. After perfusion, the animals underwent pancreas resection and preservation with the respective substance at 4 degrees C. Amylase was measured in the preservation solution after 12, 24, 36, or 48 hours. Finally, the pancreas was analyzed histologically, and a statistical analysis was performed. RESULTS: Groups SF and C showed the highest amylase levels in the preservation solution during all periods. The levels were higher than in groups C and CB (P = .05). Amylase levels were similar in groups B and CB to 24 hours (P = .05). Histological analysis was significant for analysis of pancreas islet cells and edema. Groups B and CB were histologically similar (P = .001) and different from groups SF and C. CONCLUSION: Sequential perfusion using Euro-Collins and Belzer solutions was effective for pancreas preservation in rats up to 24 hours.
Asunto(s)
Soluciones Hipertónicas , Soluciones Preservantes de Órganos , Preservación de Órganos/métodos , Páncreas , Adenosina/farmacología , Alopurinol/farmacología , Animales , Glutatión/farmacología , Soluciones Hipertónicas/farmacología , Inflamación , Insulina/farmacología , Modelos Animales , Soluciones Preservantes de Órganos/farmacología , Páncreas/efectos de los fármacos , Páncreas/fisiopatología , Rafinosa/farmacología , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
Assumptions that Shannon's concepts cannot extend to social systems are challenged by showing that social information measures the reduced uncertainties individuals have about the systemic effects of each other's behavior. The 'meaning' of individual actions is their collective consequences, which also measure reductions in systemic uncertainties about collective environments. Meaning is communicated through messages using media such as values, ethics and morals, which encourage individuals to act predictably and replicate the improbable behaviors characterizing particular societies. When individuals internalize meaning, their personal identities communicate societal priorities to future generations.