Asunto(s)
Enfermedad de Hodgkin/inmunología , Adulto , Anticuerpos Antivirales/análisis , Antígenos Virales/inmunología , Cápside/inmunología , Femenino , Herpesvirus Humano 4/inmunología , Enfermedad de Hodgkin/epidemiología , Enfermedad de Hodgkin/genética , Humanos , Masculino , Federación de RusiaRESUMEN
Lymphoblastoid cultures established from haematopoietic organs of baboons with lymphoma belong to the B-cell type. Cytoplasmic antigen is revealed in 2--6% of suspension culture cells established from lymphomatous baboons by indirect immunofluorescence reaction using serum from human Burkitt's lymphoma patients with antibody titer to capsid antigen of Epstein-Barr virus 1: 320. There is a correlation between the number of antigen-containing cells in these cultures and the number of cells in which herpes virus is revealed electron microscopically. Sera of baboons with lymphoma contain high titers of antibodies to Epstein-Barr (EBV) virus. Specific differences between antigens being produced in cultures by HBV (herpes virus of baboon) and EBV (Epstein-Barr virus) are minimal, that indicates their immunologic similarity.
Asunto(s)
Sistema Hematopoyético/inmunología , Linfoma/inmunología , Animales , Antígenos Virales/aislamiento & purificación , Células Cultivadas , Epítopos , Técnica del Anticuerpo Fluorescente , Haplorrinos , Herpesviridae/inmunología , Herpesvirus Humano 4/inmunología , Técnicas In Vitro , Papio , Receptores de Antígenos de Linfocitos B/aislamiento & purificaciónRESUMEN
The cytoplasmic antigen was determined by the indirect immunofluorescence test using serum from a Burkitt lymphoma patient with a titer of antibody to Epstein-Barr virus capsid antigen of 1.320, in the cytoplasm of 2--6% of cells of suspension cultures obtained from baboons with lymphoma. There was a correlation between the number of antigen-containing cells in this cultures and the number of cells in which herpes virus was determined by electron microscope studies. Sera from baboons with lymphoma from which the suspensions cultures had been prepared contained high titers of antibody to herpes virus of baboons (HVB) and to Epstein-Barr virus (EBV). Specific differences between the antigens produced in the cultures by HVB and EBV were minimal, indicating their immunological similarity. Lymphoblastoid cultures prepared from hemopoietic organs of baboons with lymphoma belong to B cell type.