RESUMEN
The present study was accomplished to examine and compare the effect of specific antioxidant-rich oils on hyperglycemia, dyslipidemia, renal function markers and oxidative renal damage in diabetic rats for four weeks. Papaya (P), olive (O), fenugreek (Fe), bitter gourd (B) and fish (Fi) oils were used for this purpose. Streptozotocin (STZ) was injected intraperitoneally in a single dose to induce diabetes. All oils were given orally at a dose of 3g/kg for four weeks in respective group after induction of diabetes. After treatment with oils, blood was collected, and their kidneys were stored. The level of fasting blood glucose (FBG), glycated hemoglobin (HbA1c), total cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C) and very low-density lipoprotein-cholesterol (VLDL-C) increased while amylase and high-density lipoprotein cholesterol (HDL-C) level decreased in the diabetic rats. These changes were augmented by fenugreek, bitter gourd and olive oils treatment. Diabetic rats showed elevated renal function markers in serum, including, serum creatinine (Scr), blood urea nitrogen (BUN) and alkaline phosphatase (ALP), which were restrained significantly by fenugreek and bitter gourd oil treatment. Moreover, fenugreek and bitter gourd oils treatment significantly modulated the level of thiobarbituric reactive substances (TBARS), malonaldehyde (MDA) and catalase (CAT) in the kidney of diabetic rats. The histopathological examination also showed the protective effect of these oils. The study suggests that vegetable oils are effective in reducing hyperglycemia, dyslipidemia and renal damage related to the side effects of diabetes. Thus they may have therapeutic value for preventing diabetes side effects and may be included in oil diet treatment synergically. Thus, our data suggest that oils as potent antidiabetic agent and beneficial in the control of diabetes-related abnormalities such as hyperglycemia, dyslipidemia and renal damage of STZ induced rat model of type 2 diabetes. Our study also supports the suggestion that synergistic possibilities exist concerning the use of these oils in the treatment of diabetes mellitus.
Asunto(s)
Diabetes Mellitus Experimental/tratamiento farmacológico , Aceites de Pescado/uso terapéutico , Aceites de Plantas/uso terapéutico , Animales , Glucemia/efectos de los fármacos , HDL-Colesterol/sangre , LDL-Colesterol/sangre , Diabetes Mellitus Experimental/sangre , Dislipidemias/sangre , Dislipidemias/tratamiento farmacológico , Hemoglobina Glucada/uso terapéutico , Hiperglucemia/sangre , Hiperglucemia/tratamiento farmacológico , Hipoglucemiantes/uso terapéutico , Riñón/efectos de los fármacos , Riñón/metabolismo , Masculino , Momordica charantia/química , Aceite de Oliva/uso terapéutico , Ratas , Ratas Wistar , Triglicéridos/sangre , Trigonella/químicaRESUMEN
A large variety of ornamental and decorative items are manufactured from bone waste by various unorganized sectors in India. An initial survey indicated that workers were exposed at various phases of final product. The subjects (12 industrial units) were tested for total suspended particulate matter (TSPM), particulate matter <10 microm (PM(10)), and particulate matter <2.5 microm (PM(2.5)). Prevalent levels of TSPM ranged between 2.90 and 5.89 mg m(-3). Respirable fractions of occupational dust as PM(10) and PM(2.5) were found in the range of 0.30-2.08 and 0.26-0.50 mg m(-3), respectively. Cytotoxicity study was conducted using hemolysis as a sensitive marker. In an in vitro study, rat RBCs were exposed to the concentration of 25-1,000 microg/ml for 15-120 min. A considerable variation was observed in the hemolytic activity of samples collected from different areas. At 500 microg/ml concentration, the hemolytic activity (12 h) was found to be in the range of 18-25%. Due to limitation in sample mass of respirable fractions, only one concentration (100 microg/ml/2 h) was used for comparative study on hemolysis of RBCs caused by PM(10) and PM(2.5). Interestingly, the hemolytic activity was more at PM(2.5) than PM(10) and TSPM. These results suggest that the respirable particles are capable of reaching deep into the respiratory system. The finding is significant notably when there are no standards available in occupationally exposed populations. This is the first such study. Data could be of importance to policy makers and regulators.