RESUMEN
In this study, we investigated the antioxidant activities of antheraxanthin-related carotenoids. Antheraxanthin and 9-cis-antheraxanthin were prepared from persimmon and orange fruit, respectively, and converted to other carotenoids under acidic conditions. Resulting carotenoids were purified using preparative silica gel HPLC, and their structures were analyzed in detail by NMR spectra. Both antheraxanthin and 9-cis-antheraxanthin were found to be converted to (8R)- and (8S)-mutatoxanthin at an approximate ratio of 3:2. High antioxidant activities were observed for antheraxanthin, 9-cis-antherxanthin, (8R)-mutatoxanthin, and (8S)-mutatoxanthin, with potent lipid peroxidation inhibitory and moderate 1O2-quenching activities.
Asunto(s)
Antioxidantes , Carotenoides/farmacología , Xantófilas/farmacología , Carotenoides/química , Carotenoides/aislamiento & purificación , Cromatografía Líquida de Alta Presión/métodos , Citrus sinensis/química , Depresión Química , Diospyros/química , Peroxidación de Lípido/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Conformación Molecular , Relación Estructura-Actividad , Xantófilas/química , Xantófilas/aislamiento & purificaciónRESUMEN
Accumulation of advanced glycation end-products (AGEs) is thought to contribute to muscle weakness in a diabetic animal model. Skin autofluorescence is a proposed marker for accumulation of AGEs in the skin. We aimed to investigate the relationship between AGEs accumulation, sarcopenia and muscle function of Japanese patients with type 1 diabetes. A total of 36 patients with type 1 diabetes participated in the present cross-sectional study. Sarcopenia parameters (skeletal muscle mass index and knee extension strength) were compared with subcutaneous AGEs accumulation using skin autofluorescence. The prevalence of sarcopenia and impaired knee extension strength was 16.6% (men 0.0%, women 22.2%) and 47.2% (men 22.2%, women 55.6%), respectively. Knee extension strength was negatively correlated with skin autofluorescence (r² = 0.14, P < 0.05), but not with skeletal muscle mass index. In conclusion, the AGEs accumulation might be one of the reasons of impaired lower limb muscle function in Japanese patients with type 1 diabetes.
Asunto(s)
Diabetes Mellitus Tipo 1/complicaciones , Productos Finales de Glicación Avanzada/metabolismo , Debilidad Muscular/complicaciones , Debilidad Muscular/metabolismo , Sarcopenia/complicaciones , Sarcopenia/metabolismo , Pueblo Asiatico , Estudios Transversales , Diabetes Mellitus Tipo 1/epidemiología , Femenino , Humanos , Japón , Masculino , Persona de Mediana Edad , Debilidad Muscular/epidemiología , Factores de Riesgo , Sarcopenia/epidemiologíaRESUMEN
Violaxanthin and 9-cis-violaxanthin (major epoxycarotenoids in fruit) were prepared from mango fruit, purified, and converted to other carotenoids under acidic conditions. The resulting carotenoid structures were then analyzed in detail. Not only violaxanthin but also 9-cis-violaxanthin were found to be converted to (8S,8'S)-, (8S,8'R)-, and (8R,8'R)-auroxanthin at an approximate ratio of 4:6:1. Antioxidant activities of violaxanthin, 9-cis-violaxanthin, (8S,8'S)-auroxanthin, and (8S,8'R)-auroxanthin were examined. They possessed potent lipid peroxidation inhibitory and very weak 1O2 quenching activities.