RESUMEN
Industrial production of plague monoclonal antibodies in mice BALB/c was shown possible. Precipitation of the immunoglobulins with caprylic acid provided the highest purity of the preparations. Maintenance of the specific activity by the monoclonal antibodies was stable. The immunoenzymatic conjugate and erythrocytic diagnosticum on the basis of the monoclonal antibodies are sensitive, specific and may be used in antiplague practice.
Asunto(s)
Anticuerpos Monoclonales , Yersinia pestis/inmunología , Animales , Anticuerpos Monoclonales/aislamiento & purificación , Hibridomas/inmunología , Ratones , Ratones Endogámicos BALB CRESUMEN
It is recommended that the Hottinger agar medium with polymyxin or erythromycin be used for isolation of pure cultures of the plague microbe from specimens containing various species of microorganisms, except Proteus. Such a procedure was used in the Kizil-Kum for the bacteriological examinations with respect to plague. The procedure may be used in the natural foci with the strains resistant to these antibiotics.
Asunto(s)
Eritromicina/farmacología , Polimixinas/farmacología , Yersinia pestis/aislamiento & purificación , Animales , Técnicas Bacteriológicas , Clima Desértico , Roedores/microbiología , Roedores/parasitología , UzbekistánRESUMEN
Investigation of the response to polymyxin of 65 strains of Pasteurella isolated from various foci and 46 their back mutants showed that all of them were usually highly resistant to polymyxin (MIC 200--500 micrograms/ml). The Pasteurella strains isolated in the Gissaro-Darvaz natural focus, Turkey and Congo were highly sensitive to polymyxin (MIC 10--25 micrograms/ml). Single cultures highly sensitive to the antibiotic were detected among the polymyxin-resistant strains. Polymyxin-sensitive mutants of these cultures with lowered requirements in the growth factors were obtained.