RESUMEN

The aim of the study was to demonstrate the role of parathyroid hormone related protein (PTHrP) in stimulating aldo-keto reductase (AKR) 1C3 expression in prostate cancer (CaP) cells. CaP cell proliferation and resistance to apoptosis was increased by PTHrP transfection. Conversely, reducing AKR1C3 expression by siRNA decreased cell proliferation. Since these effects could be mediated through AKR1C3-catalyzed reductions of the PPARγ ligand, 15-DeoxyΔ(12,14)-PGJ(2), we treated the cells with prostaglandins (PG). (PG) D(2) inhibited cell proliferation, but its metabolite, 9α,11ß-PGF(2), did not effect CaP cell growth. The AKR1C family members serve as potential therapeutic targets for CaP therapy.

Asunto(s)

3-Hidroxiesteroide Deshidrogenasas/metabolismo , Regulación Neoplásica de la Expresión Génica , Hidroxiprostaglandina Deshidrogenasas/metabolismo , Proteína Relacionada con la Hormona Paratiroidea/metabolismo , Neoplasias de la Próstata/metabolismo , Miembro C3 de la Familia 1 de las Aldo-Ceto Reductasas , Andrógenos/metabolismo , Apoptosis , Línea Celular Tumoral , Proliferación Celular , Humanos , Masculino , Modelos Biológicos , Reacción en Cadena de la Polimerasa/métodos , Prostaglandinas/metabolismo , Transfección , Regulación hacia Arriba