RESUMEN

The mitogenic effect of Cratylia mollis seed lectin preparations containing two (Cramoll 1,4) or one molecular form (Cramoll 1) showed activity similar to the well known T-cell mitogen, concanavalin A (Con A). The effect on human lymphocytes was analyzed through a colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). Inhibition of lymphocyte proliferation with methyl-alpha-d-mannoside (both preparations) indicated that the mitogenic effect involved carbohydrate lectin binding sites.

Asunto(s)

Lectinas/metabolismo , Linfocitos/efectos de los fármacos , Plantas/metabolismo , Sitios de Unión , Carbohidratos/química , División Celular , Colorantes/farmacología , Concanavalina A/química , Relación Dosis-Respuesta a Droga , Humanos , Lectinas/química , Activación de Linfocitos , Linfocitos/metabolismo , Sales de Tetrazolio/farmacología , Tiazoles/farmacología , Factores de Tiempo