Asunto(s)
Hipopigmentación/diagnóstico , Lentigo/diagnóstico , Nevo/diagnóstico , Neoplasias Cutáneas/diagnóstico , Adulto , Diagnóstico Diferencial , Humanos , Hipopigmentación/congénito , Hipopigmentación/patología , Lentigo/congénito , Lentigo/patología , Masculino , Nevo/congénito , Nevo/patología , Neoplasias Cutáneas/congénito , Neoplasias Cutáneas/patologíaRESUMEN
Cytokeratins (CKs) are expressed specifically in the cytoplasm of epithelial cells. We investigated the expression of CKs immunohistochemically in basal cell carcinomas (BCCs), epidermis overlying tumour, and skin tumor-associated amyloidosis (STA). Twenty cases of BCC, 11 of which had STA were included to the study. The primary antibodies of CK1-8 (AE3), CK10 (DEK-10), CK14 (LL002), CK17 (E3), CK18 (DC10), CK19 (KS19.1), CK 5/6/18 (LP34), CK8/18 (5D3) were applied to the section immunohistochemically. In BCCs without STA, CK1-8, CK14 and CK17 antibodies were expressed by tumour tissue in all biopsy specimens. In the BCCs with STA, tumour tissue was immunoreactive always with CK1-8 and CK17 antibodies, and commonly immunoreactive with anti-CK 14 antibody. In the epidermis overlying tumour tissue, there was positive immunoreactivity with anti-CK 1-8, CK 5/6/18, CK 10 and CK 14 antibodies in all biopsy specimens. Anti-CK 17 antibody was also positive in 17 biopsy specimens. STA is immunoreactive with anti-CK1-8 in all specimens. There was mild staining with anti-CK5/6/18 and with anti-CK19 whereas no immunoreactivity with anti-CK10 and CK18 antibodies was found. In conclusion, we could not find a significant CK expression difference between BCCs with and without STA. Weak positivity and a few number of CKs were shown in STA when compared with those of BCC and epidermis overlying tumour tissue expressing the more variable CKs. Interestingly, although CKs coexpressed in pairs consisting of one basic and one acidic CK, we detected predominantly basic CKs in STA.
Asunto(s)
Amiloidosis/metabolismo , Carcinoma Basocelular/metabolismo , Epidermis/metabolismo , Queratinas/metabolismo , Neoplasias Cutáneas/metabolismo , Células del Estroma/metabolismo , Carcinoma Basocelular/patología , Epidermis/patología , Humanos , Técnicas para Inmunoenzimas , Queratinas/clasificación , Neoplasias Cutáneas/patología , Células del Estroma/patologíaRESUMEN
BACKGROUND: Heat shock proteins (HSPs) are expressed by most living cells and play fundamental roles in many biological processes. Their synthesis increases by a variety of stresses in order to enable cellular survival. Although it is known that they play an important role in immune and inflammatory responses of the skin, the role of HSPs in the pathogenesis of skin diseases has been studied in only limited skin diseases. Lichen planus (LP) is a relatively common papulosquamous dermatosis, and cell-mediated immunity plays an important role in its pathogenesis. Although an altered expression of certain HSPs was reported in oral LP lesions, the expression of HSPs in cutaneous lesions of LP has not been investigated. In this immunohistochemical study, we aimed at investigating the role of HSPs in the pathogenesis of LP by studying whether there is any difference in HSP expression in cutaneous lesions of LP when compared to normal skin and psoriasis vulgaris (PV). METHODS: Formalin-fixed paraffin-embedded skin biopsy specimen blocks from LP patients (n = 39), patients with psoriasis (n = 20), and normal skin controls (n = 20) were used in the study. Antibodies to HSPs 60 and 70 were applied immunohistochemically by using streptavidin-biotin-horseradish peroxidase complex. An immunoreactivity intensity distribution index (IRIDI) was calculated to express the proportion of the immunoreactive cells as well as the staining intensity in different layers of the epidermis. RESULTS: The mean IRIDI scores for HSP60 expression in the basal, suprabasal, and superficial epidermal layers of cutaneous LP were moderately higher than those of normal skin, but not different from those of PV skin. These scores for HSP70 in lesions of LP were moderately lower than those for normal skin in the basal layer, but not significantly different from normal in the other two layers. Scores for HSP70 in PV lesions were markedly lower in all three layers. In the cells of the inflammatory infiltrates (mostly lymphocytes), HSP60 scores for LP were moderately higher, compared to those for PV, whereas scores for HSP70 were much lower for LP and very much lower for PV. CONCLUSIONS: Significantly altered levels of HSP proteins were found in cutaneous LP lesions in comparison with normal skin and psoriasis, suggesting the role of HSPs in the pathogenesis of LP.
Asunto(s)
Chaperonina 60/biosíntesis , Epidermis/metabolismo , Proteínas HSP70 de Choque Térmico/biosíntesis , Liquen Plano/metabolismo , Psoriasis/metabolismo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Inmunohistoquímica , Queratinocitos/metabolismo , Queratinocitos/patología , Liquen Plano/patología , Masculino , Persona de Mediana Edad , Psoriasis/patologíaRESUMEN
Melkersson-Rosenthal (MRS) syndrome is characterized by a classical triad of recurrent or persistent orofacial swelling, peripheral facial nerve paralysis and lingua plicata. Granulomatous cheilitis (GC) is regarded as a monosymptomatic form of MRS. The exact aetiologies of MRS and GC are unknown. In this study we investigated the possible role of mycobacteria in these two conditions. A ribosomal RNA amplification-based Gen-Probe amplified Mycobacterium tuberculosis direct test was used to investigate the presence of M. tuberculosis complex in paraffin-embedded skin biopsy specimens from five patients with MRS and one patient with GC. Three of the six specimens were shown to be positive using this system; one of the positive specimens also showed positive Ziehl-Neelsen staining. These results suggest a possible mycobacterial aetiology for MRS and GC.
Asunto(s)
Síndrome de Melkersson-Rosenthal/microbiología , Mycobacterium tuberculosis/fisiología , Adolescente , Adulto , Biopsia , Sondas de ADN , Edema/patología , Femenino , Estudios de Seguimiento , Amplificación de Genes , Humanos , Leucocitos Mononucleares/patología , Masculino , Síndrome de Melkersson-Rosenthal/patología , Persona de Mediana Edad , Mycobacterium tuberculosis/genética , ARN Ribosómico 16S/análisis , Piel/microbiología , Piel/patologíaRESUMEN
Both clinically and histopathologically, melanoma of childhood is a rarely encountered lesion. In addition, it has particular histopathologic diagnostic problems. Differential diagnosis of this lesion and Spitz nevus is at times very problematic, in that distant metastases and death of the patient may be the only diagnostic criteria for some cases. We present a 4-year-old girl with an atypical melanocytic neoplasm with Spitzoid features on the left subscapular region.
Asunto(s)
Melanoma/patología , Nevo de Células Epitelioides y Fusiformes/patología , Neoplasias Cutáneas/patología , Preescolar , Diagnóstico Diferencial , Femenino , Humanos , Metástasis de la NeoplasiaRESUMEN
Nevoid hyperkeratosis of the nipple and areola, which is characterized by verrucous thickening and pigmentation of the nipple or areola, is a rare condition. Different therapeutic options have been used with varying results, but there is no uniformly effective treatment. We describe two patients with hyperkeratosis of the nipple and areola who responded well to topical calcipotriol ointment.
Asunto(s)
Antineoplásicos/uso terapéutico , Calcitriol/análogos & derivados , Calcitriol/uso terapéutico , Nevo Pigmentado/tratamiento farmacológico , Neoplasias Cutáneas/tratamiento farmacológico , Administración Cutánea , Adulto , Antineoplásicos/administración & dosificación , Calcitriol/administración & dosificación , Femenino , Humanos , Masculino , Nevo Pigmentado/patología , Pezones , Neoplasias Cutáneas/patologíaRESUMEN
AIM: The aim of this study is to have a further insight into this vascular tumor both clinically and histopathologically. METHODS: We examined 6 cases of acral arteriovenous tumor. Histochemically Masson trichrom, elastic van Gieson and immunohistochemically Desmin, Factor VIII, S100 and Mast cell tryptase were applied to the paraffin sections to evaluate the content of the vessels and mast cell distribution within the lesion. RESULTS: Vessels had smooth muscle and laminae elastica in most of the lesions. Arteriovenous anastomosis and glomus cells were not detected in any of the lesions. Mast cell count in the lesion was 215.17+/-42.2 whereas in control cases it was 89.16+/- 28.44. (p<0.001) CONCLUSIONS: We suggest that acral arteriovenous tumor is an arterial and venous originated neoplasm, not an embryonic remnant or an aneurysm.