RESUMEN

Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC.

Asunto(s)

Lactobacillus/aislamiento & purificación , Vagina/microbiología , Excreción Vaginal/microbiología , Vaginosis Bacteriana/microbiología , Análisis de Varianza , Brasil/epidemiología , Candidiasis Vulvovaginal/microbiología , Distribución de Chi-Cuadrado , ADN Bacteriano/aislamiento & purificación , ADN Espaciador Ribosómico/aislamiento & purificación , Electroforesis en Gel de Campo Pulsado , Femenino , Humanos , Peróxido de Hidrógeno/metabolismo , Lactobacillus/clasificación , Lactobacillus/metabolismo , Reacción en Cadena de la Polimerasa , Vaginosis Bacteriana/epidemiología