RESUMEN
The emerging biomedical applications of selenium nanoparticles (SeNPs) require facile and efficient strategy to assess its interactions with cell membrane. In this study, an efficient and reproducible microwave assisted method was used to synthesize SeNPs with controllable size distributions. The physical properties of the emergent structures, such as morphology, structure, and size were studied. The antimicrobial applications of SeNPs were assessed by electrochemical analyses that entailed the systematic acquisition of cyclic voltammetry data. Our results demonstrate a straightforward method to predict the integrity of bacterial cell membranes following the administration of SeNP treatments.
RESUMEN
We report the development of effective drug loaded nanocarriers to combat multidrug resistant infection especially in case of osteomyelitis. The hollow mesoporous hydroxyapatite nanoparticles (hmHANPs) and solid/non-hollow hydroxyapatite nanoparticles (sHANPs) were synthesized by core-shell and co-precipitation techniques respectively. High encapsulation of the drug (ciprofloxacin) was observed in hmHANPs as compared to sHANPs, which may be due to the hollow porous structure of hmHANPs. These nanoparticles were characterized by scanning electron microscope (FESEM), N2 adsorption/desorption, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Thermogravimetric analysis (TGA). Approximately 80% of the encapsulated drug was released at pH 4.5 within 5â¯days in case of hmHANPs while at pH 7.4, a sustained drug release profile was obtained and only 48.73% of the drug was released after 9â¯days. The results of kinetic drug release revealed that drug loaded hmHANPs showed fickian diffusion and anomalous drug diffusion mechanism at pH 4.5 and 7.4 respectively. Owing to their porous structure and high drug loading capacity, hmHANPs showed enhanced antibacterial activity against Staphylococcus aureus and Escherichia coli (drug resistant strains of osteomyelitis) in comparison to that with sHANPs. In addition, hmHANPs showed a pH sensitive drug release profile, high surface area (105.33â¯m2/g) with increased pore volume (0.533â¯cm3/g) and superior antimicrobial activity against osteomyelitis as compared to sHANPs.