RESUMEN
We undertook analysis of clinical and instrumental features of gallbladder pathology in patients with a weight deficit for the elucidation of peculiarities of eating behavior, blood leptin level, and cytokine content of gastric biopsies. Underweight patients with inflammatory and dysfunctional diseases of gallbladder more frequently than others presented with abdominal pain syndrome. All patients enrolled in the study showed every type of eating disorders with the predominance of the limiting behavior. Weight deficit in patients with chronic cholecystitis was associated with hyperleptinemia and increased production of proinflammatory cytokines.
Asunto(s)
Trastornos de Alimentación y de la Ingestión de Alimentos/patología , Enfermedades de la Vesícula Biliar/patología , Delgadez/patología , Adulto , Citocinas/biosíntesis , Citocinas/sangre , Trastornos de Alimentación y de la Ingestión de Alimentos/sangre , Trastornos de Alimentación y de la Ingestión de Alimentos/diagnóstico por imagen , Femenino , Enfermedades de la Vesícula Biliar/sangre , Enfermedades de la Vesícula Biliar/diagnóstico por imagen , Humanos , Leptina/biosíntesis , Leptina/sangre , Masculino , Delgadez/sangre , Delgadez/diagnóstico por imagen , UltrasonografíaAsunto(s)
Proteínas de Escherichia coli/metabolismo , Escherichia coli/metabolismo , Fosfolípidos/fisiología , Secuencia de Aminoácidos , Secuencia de Bases , Membrana Celular/química , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/genética , Datos de Secuencia Molecular , Operón , Fosfolípidos/química , Regiones Promotoras Genéticas , Transducción de SeñalRESUMEN
The secretion of alkaline phosphatase (PhoA) and peculiarities of biogenesis of the cell envelope were studied in Escherichia coli strains HD30/pHD 102 and HDL11 with controlled synthesis of anionic phospholipids, phosphatidylglycerol, and cardiolipin. Inactivation of the pgsA gene encoding the synthesis of anionic phospholipids or changes in the regulation of its expression by an environmental factor caused changes in the metabolism and composition of membrane phospholipids, which resulted in a decrease in the secretion of alkaline phosphatase through the cytoplasmic membrane and an increase in PhoA secretion from the periplasm into the culture medium. A conforming increase was observed in exopolysaccharide secretion, as well as a decrease in the contents of lipopolysaccharide and lipopolyprotein of the outer membrane that determine the membrane barrier properties. The results obtained testify that anionic phospholipids play a significant role in protein secretion and are probably involved in the interrelation between the protein secretion and biogenesis of cell envelope components.
Asunto(s)
Proteínas de la Membrana Bacteriana Externa/biosíntesis , Membrana Celular/metabolismo , Proteínas de Escherichia coli/biosíntesis , Escherichia coli/metabolismo , Fosfolípidos/metabolismo , Fosfatasa Alcalina/biosíntesis , Cardiolipinas/biosíntesis , Membrana Celular/química , Pared Celular/metabolismo , Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica , Periplasma/metabolismo , Fosfatidilgliceroles/biosíntesis , Fosfolípidos/química , Mutación Puntual , Transferasas (Grupos de Otros Fosfatos Sustitutos)/genéticaRESUMEN
Activity of phosphate PPHO or arabinose PBAD promoter of Escherichia coli has been studied depending on the content of zwitter-ionic phosphatidylethanolamine (PE) and anionic phospholipids in membranes. In the absence of PE or under significant decrease in the content of anionic phospholipids, there is a significant decline of PPHO promoter activity but not PBAD promoter. Since the PPHO promoter belongs to the Pho-regulon--a member of the family of two-component regulatory systems of signal transduction having membrane sensors, the regulation of gene expression by phospholipids is presumed to be realized through a membrane sensor.
Asunto(s)
Escherichia coli/metabolismo , Lípidos de la Membrana/metabolismo , Fosfolípidos/metabolismo , Regiones Promotoras Genéticas , Secuencia de Bases , ADN Bacteriano , Escherichia coli/genética , Datos de Secuencia Molecular , Transducción de SeñalRESUMEN
Inactivation of pgsA, which is responsible for biosynthesis of anionic phospholipid phosphatidylglycerol (PG), was shown to affect biosynthesis and secretion of alkaline phosphatase (PhoA) in Escherichia coli. A decrease in PG, but not in total anionic phospholipids, correlated with reduction of PhoA secretion, suggesting the role of PG in this process. A dramatic decrease in PG (from 18 to 3, but not 8, percent of the total phospholipids) inhibited not only secretion, but also synthesis of PhoA. In addition, pgsA inactivation expedited repression of PhoA synthesis by exogenous orthophosphate.