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In the field of image processing, noise represents an unwanted component that can occur during signal acquisition, transmission, and storage. In this paper, we introduce an efficient method that incorporates redescending M-estimators within the framework of Wiener estimation. The proposed approach effectively suppresses impulsive, additive, and multiplicative noise across varied densities. Our proposed filter operates on both grayscale and color images; it uses local information obtained from the Wiener filter and robust outlier rejection based on Insha and Hampel's tripartite redescending influence functions. The effectiveness of the proposed method is verified through qualitative and quantitative results, using metrics such as PSNR, MAE, and SSIM.
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The voltage-dependent anion channel (VDAC) is the most abundant protein in the outer mitochondrial membrane (OMM) of all eukaryotes, having an important role in the communication between mitochondria and cytosol. The plant VDAC family consists of a wide variety of members that may participate in cell responses to several environmental stresses. However, there is no experimental information about the members comprising the maize VDAC (ZmVDAC) family. In this study, the ZmVDAC family was identified, and described, and its gene transcription profile was explored during the first six days of germination and under different biotic stress stimuli. Nine members were proposed as bona fide VDAC genes with a high potential to code functional VDAC proteins. Each member of the ZmVDAC family was characterized in silico, and nomenclature was proposed according to phylogenetic relationships. Transcript levels in coleoptiles showed a different pattern of expression for each ZmVDAC gene, suggesting specific roles for each one during seedling development. This expression profile changed under Fusarium verticillioides infection and salicylic acid, methyl jasmonate, and gibberellic acid treatments, suggesting no redundancy for the nine ZmVDAC genes and, thus, probably specific and diverse functions according to plant needs and environmental conditions. Nevertheless, ZmVDAC4b was significantly upregulated upon biotic stress signals, suggesting this gene's potential role during the biotic stress response.
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Introduction: Cardiovascular diseases are responsible for a significant morbimortality rate around the world. Due to the characteristics of their work, health care professionals, including veterinarians, are more prone to present this type of pathology. Objectives: To determine the level of cardiovascular risk using different scales in a group of veterinarians. Methods: A descriptive and cross-sectional study of 610 Spanish veterinarians was conducted to assess cardiovascular risk scores, including 14 overweight and obesity scales, six fatty liver scales, six cardiovascular risk scales, four atherogenic indices, and three metabolic syndrome scales. Results: The prevalence of obesity among women was 7.95%, and 17.53% among men. Hypertension was present in 15.23% of women and 24.68% of men. Dyslipidemia affected 45% of women and 58.64% of men. The prevalence of metabolic syndrome according to the International Diabetes Federation criteria was slightly over 10% while 10.90% of women and 14.93% of men showed moderate to high values on the Registre Gironí del Cor scale. Conclusions: There is a moderate to high level of cardiovascular risk among veterinarians in this group.
Introdução: As doenças cardiovasculares são responsáveis por uma taxa de morbimortalidade significativa no mundo. Devido às características do seu trabalho, os profissionais de saúde, incluindo veterinários, são mais propensos a apresentar este tipo de patologia. Objetivos: Determinar o nível de risco cardiovascular utilizando diferentes escalas num grupo veterinários. Métodos: Estudo descritivo e transversal em 610 veterinários em que foram avaliadas diferentes escalas relacionadas com o risco cardiovascular, incluindo 14 escalas de excesso de peso e obesidade, seis de fígado gordo, seis de risco cardiovascular, quatro índices aterogênicos e três de síndrome metabólica. Resultados: A prevalência da obesidade foi de 7,95% nas mulheres e 17,53% nos homens. A hipertensão estava presente em 15,23% das mulheres e 24,68% dos homens. A dislipidemia afetou 45% das mulheres e 58,64% dos homens. A prevalência da síndrome metabólica com critérios da International Diabetes Federation foi ligeiramente superior a 10% enquanto valores moderados ou elevados da escala Registre Gironí del Cor foram encontrados em 10,90% das mulheres e 14,93% dos homens. Conclusões: Neste grupo de veterinários, o nível de risco cardiovascular pode ser considerado de moderado a elevado.
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Plants being sessile organisms are exposed to various biotic and abiotic factors, thus causing stress. The Pseudomonas aeruginosa bacterium is an opportunistic pathogen for animals, insects, and plants. Direct exposure of Arabidopsis thaliana to the P. aeruginosa PAO1 strain induces plant death by producing a wide variety of virulence factors, which are regulated mainly by quorum sensing systems. Besides virulence factors, P. aeruginosa PAO1 also produces cyclodipeptides (CDPs), which possess auxin-like activity and promote plant growth through activation of the target of the rapamycin (AtTOR) pathway. On the other hand, plant defense mechanisms are regulated through the production of phytohormones, such as salicylic acid (SA) and jasmonic acid (JA), which are induced in response to pathogen-associated molecular patterns (PAMPs), activating defense genes associated with SA and JA such as PATHOGENESIS-RELATED-1 (PR-1) and LIPOXYGENASE2 (LOX2), respectively. PR proteins are suggested to play critical roles in coordinating the Systemic Acquired Resistance (SAR). In contrast, LOX proteins (LOX2, LOX3, and LOX4) have been associated with the production of JA by producing its precursors, oxylipins. The activation of defense mechanisms involves signaling cascades such as Mitogen-Activated Protein Kinases (MAPKs) or the TOR pathway as a switch for re-directing energy towards defense or growth. In this work, we challenged A. thaliana (wild type, mpk6 or mpk3 mutants, and overexpressing TOR) seedlings with P. aeruginosa PAO1 strains to identify the role of bacterial CDPs in the plant immune response. Results showed that the pre-exposure of these Arabidopsis seedlings to CDPs significantly reduced plant infection of the pathogenic P. aeruginosa PAO1 strains, indicating that plants that over-express AtTOR or lack MPK3/MPK6 protein-kinases are more susceptible to the pathogenic effects. In addition, CDPs induced the GUS activity only in the LOX2::GUS plants, indicative of JA-signaling activation. Our findings indicate that the CDPs are molecules that trigger SA-independent and JA-dependent defense responses in A. thaliana; hence, bacterial CDPs may be considered elicitors of the Arabidopsis immune response to pathogens.
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Proteínas de Arabidopsis , Arabidopsis , Animales , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Ciclopentanos/metabolismo , Inmunidad , Oxilipinas/metabolismo , Desarrollo de la Planta , Enfermedades de las Plantas/microbiología , Pseudomonas aeruginosa , Ácido Salicílico/metabolismo , Factores de Virulencia/metabolismo , Factores de Virulencia/farmacologíaRESUMEN
Insulin resistance is a pathological entity that can lead to alterations in lipid metabolism and can increase cardiovascular risk. Objective. The aim of this study was to assess the influence of different sociodemographic variables such as age, sex and social class and healthy habits such as smoking, physical activity and adherence to the Mediterranean diet on the cardiometabolic profile of Spanish workers. Material and methods. A descriptive, cross-sectional study was carried out in 1457 Spanish workers in an attempt to evaluate the effect of healthy habits (physical exercise determined with the IPAQ questionnaire, Mediterranean diet and tobacco consumption) and sociodemographic variables (age, sex and social class) on the values of different insulin resistance scales. Results. The progressive increase in the level of physical activity and high adherence to the Mediterranean diet achieved an improvement in the mean values and in the prevalence of elevated values in all the insulin resistance scales analyzed in this study. Age over 50 years and belonging to the least favored social classes (social classes II-III) were the variables that increased the risk of presenting insulin resistance. Male sex also increased the risk of presenting insulin resistance. Conclusions. The different healthy habits such as vigorous physical exercise and high adherence to the Mediterranean diet improve the values of the different scales that assess insulin resistance(AU)
La resistencia a la insulina es una entidad patológica que puede provocar alteraciones en el metabolismo de los lípidos y puede aumentar el riesgo cardiovascular. Objetivo. En este trabajo se pretende valorar la influencia de diferentes variables sociodemográficas como la edad, el sexo y la clase social y hábitos saludables como el consumo de tabaco, la actividad física y la adherencia a la dieta mediterránea en el perfil cardiometabólico de trabajadores españoles. Material y métodos. Se realizó un estudio descriptivo y transversal en 1457 trabajadores españoles intentando evaluar el efecto de los hábitos saludables (ejercicio físico determinado con el cuestionario IPAQ, dieta mediterránea y consumo de tabaco) y las variables sociodemográficas (edad, sexo y clase social) sobre los valores de diferentes escalas de resistencia a la insulina. Resultados. El aumento progresivo del nivel de actividad física y la alta adherencia a la dieta mediterránea consiguieron una mejoría en los valores medios y en la prevalencia de los valores elevados en todas las escalas de resistencia a la insulina analizadas en este estudio. La edad por encima de los 50 años y la pertenencia a las clases sociales menos favorecidas (clases sociales II-III) fueron las variables que aumentaron el riesgo de presentar resistencia a la insulina. El sexo masculino también incrementó el riesgo de presentar resistencia a la insulina. Conclusiones. Los diferentes hábitos saludables como el ejercicio físico vigoroso y la alta adherencia a la dieta mediterránea mejoran los valores de las diferentes escalas que valoran resistencia a la insulina(AU)
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Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Anciano , Resistencia a la Insulina , Síndrome Metabólico/complicaciones , Dieta Mediterránea , Metabolismo de los Lípidos , Estilo de Vida Saludable , Factores de Riesgo Cardiometabólico , Clase Social , Pesos y Medidas , Ejercicio Físico , Evaluación Nutricional , Circunferencia Abdominal , Conducta AlimentariaRESUMEN
INTRODUCTION: In 1993, Cuba achieved leprosy elimination according to the World Health Organization's (WHO) indicator of less than one case per 10,000 population. Despite this achievement, detection of new cases occurs every year among all age groups including children. Detection of new cases in children reveals persistent transmission of the infection. OBJECTIVE: To describe the clinical and epidemiological features of leprosy in individuals younger than 15 years (childhood leprosy) reported to the Cuban National Leprosy Control Program (NLCP) between 2012 and 2019. METHODS: We conducted a retrospective descriptive study between 2012 and 2019 to assess the clinical and epidemiologic features of individuals under the age of 15 years with a confirmed diagnosis of leprosy reported to the NLCP. We reviewed the NLCP database and collected data to better define the total number of cases of leprosy in adults, children (younger than 15 years). We assessed socio-demographic variables (age, gender, and province of residence) as well as variables of clinical interest including operational classification and staging at diagnosis, bacillary index, grade of disability by WHO staging. Additionally, we evaluated epidemiological variables including passive versus active surveillance of cases, contact investigation focusing specifically in household transmission, and the degree of kinship as well as standing of the child within the focus of transmission when there were additional cases. RESULTS: We identified fifty children during the study period corresponding to 3% of the overall cases of leprosy comprising all age groups in Cuba. In the age group younger than 15 years, the majorities of cases was from the Granma province and most were between the ages of 10 and 14 years. Clinically, multibacillary/lepromatous forms were the most common type identified with positive bacillary index. The majority of children diagnosed with leprosy during our study period had a history of a relative with a confirmed diagnosis of leprosy. CONCLUSIONS: Detection of cases of leprosy in individuals younger than 15 years of age in Cuba demonstrates ongoing transmission of M. leprae in specific geographic hotspots. Its frequency in the early adolescence, the predominant clinical forms, and the mode of detection associated with sources of suspected familiar infection demonstrated that there is a need for further efforts by the NLCP to conduct active surveillance activities among affected communities to identify cases of leprosy earlier with the goal of preventing further household and community transmission.
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Lepra/epidemiología , Adolescente , Niño , Preescolar , Trazado de Contacto , Cuba/epidemiología , Femenino , Humanos , Lactante , Lepra/diagnóstico , Lepra/microbiología , Masculino , Mycobacterium leprae/genética , Mycobacterium leprae/fisiología , Estudios RetrospectivosRESUMEN
Resumen: Objetivo: Analizar la asociación entre la exposición crónica a contaminantes atmosféricos y la tasa de mortalidad por Covid-19 en ciudades mexicanas. Material y métodos: Estudio ecológico en 25 ciudades mexicanas utilizando el reporte de casos diarios de muertes por Covid-19 (febrero a junio 2020) y datos validados de contaminantes atmosféricos, considerando concentraciones promedio en cada ciudad en el último año. Se utilizaron modelos de regresión Poisson, con modelos aditivos generalizados y variables de ajuste. Resultados: Se encontró un incremento significativo de 3.5% (IC95% 2.3-4.7) en la tasa de mortalidad por Covid-19 por incremento de 1µg/m3 de NO2. La asociación con PM2.5 fue no significativa, con un incremento de 1.8% por cada µg/m3. Conclusiones: Los resultados sugieren una asociación entre la mortalidad por Covid-19 y la exposición a NO2. Esta primera aproximación del riesgo asociado con la contaminación del aire requiere de análisis más precisos, pero es consistente con estudios de otras regiones.
Abstract Objective: To analyze the relationship between chronic exposures to air pollution with Covid-19 death rate in Mexican cities. Materials and methods: Ecological study in 25 Mexican cities using the report of daily Covid-19 deaths (from February to June 2020) and validated data of air pollutants, considering average concentrations in each city for the last year. Poisson regression models using generalized additive models with adjustment variables (GAM) were used. Results: A significant increase of 3.5% (95% CI 2.3-4.7) was found in Covid-19 death rate for each 1µg/m3 in annual concentration of NO2. The association with PM2.5 was not significant, with an increase of 1.8% for each 1µg/m3. Conclusions: Results suggest an association between Covid-19 mortality and chronic exposure to NO2. This first approximation of the risk associated with air pollution requires a more precise analysis, but is consistent with what was observed in other studies.
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Cold and freezing stresses severely affect plant growth, development, and survival rate. Some plant species have evolved a process known as cold acclimation, in which plants exposed to temperatures above 0 °C trigger biochemical and physiological changes to survive freezing. During this response, several signaling events are mediated by transducers, such as mitogen activated protein kinase (MAPK) cascades. Plasma membrane H+-ATPase is a key enzyme for the plant cell life under regular and stress conditions. Using wild type and mpk3 and mpk6 knock out mutants in Arabidopsis thaliana, we explored the transcriptional, translational, and 14-3-3 protein regulation of the plasma membrane H+-ATPase activity under the acclimation process. The kinetic analysis revealed a differential profiling of the H+-ATPase activity depending on the presence or absence of MPK3 or MPK6 under non-acclimated or acclimated conditions. Negative regulation of the plasma membrane H+-ATPase activity was found to be exerted by MPK3 in non-acclimated conditions and by MPK6 in acclimated conditions, describing a novel form of regulation of this master ATPase. The MPK6 regulation involved changes in plasma membrane fluidity. Moreover, our results indicated that MPK6 is a critical regulator in the process of cold acclimation that leads to freezing tolerance and further survival.
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Aclimatación/fisiología , Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Arabidopsis/fisiología , Membrana Celular/enzimología , Frío , Proteínas Quinasas Activadas por Mitógenos/metabolismo , ATPasas de Translocación de Protón/metabolismo , Congelación , Cinética , Fluidez de la Membrana , Biosíntesis de Proteínas , Transcripción GenéticaRESUMEN
Objetivo. Analizar la asociación entre la exposición crónica a contaminantes atmosféricos y la tasa de mortalidad por Covid-19 en ciudades mexicanas. Material y métodos. Estudio ecológico en 25 ciudades mexicanas utilizando el reporte de casos diarios de muertes por Covid-19 (febrero a junio 2020) y datos validados de contaminantes atmosféricos, considerando concentraciones promedio en cada ciudad en el último año. Se utilizaron modelos de regresión Poisson, con modelos aditivos generalizados y variables de ajuste. Resultados. Se encontró un incremento significativo de 3.5% (IC95% 2.3-4.7) en la tasa de mortalidad por Covid-19 por incremento de 1µg/m3 de NO2. La asociación con PM2.5 fue no significativa, con un incremento de 1.8% por cada µg/ m3. Conclusiones. Los resultados sugieren una asociación entre la mortalidad por Covid-19 y la exposición a NO2. Esta primera aproximación del riesgo asociado con la contami-nación del aire requiere de análisis más precisos, pero es consistente con estudios de otras regiones.
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Objective. To analyze the relationship between chronic exposures to air pollution with Covid-19 death rate in Mexican cities. Materials and methods. Ecological study in 25 Mexican cities using the report of daily Covid-19 deaths (from February to June 2020) and validated data of air pollutants, considering average concentrations in each city for the last year. Poisson regression models using generalized additive models with adjustment variables (GAM) were used. Results. A significant increase of 3.5% (95% CI 2.3-4.7) was found in Covid-19 death rate for each 1µg/m3 in annual concentration of NO2. The association with PM2.5 was not significant, with an increase of 1.8% for each 1µg/m3. Conclusions. Results suggest an association between Covid-19 mortality and chronic exposure to NO2. This first approximation of the risk associated with air pollution requires a more precise analysis, but is consistent with what was observed in other studies.
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Contaminantes Atmosféricos , Contaminación del Aire , COVID-19 , Contaminantes Atmosféricos/análisis , Contaminantes Atmosféricos/toxicidad , Contaminación del Aire/estadística & datos numéricos , Ciudades/epidemiología , Exposición a Riesgos Ambientales/estadística & datos numéricos , Humanos , Mortalidad , Material Particulado/análisis , Material Particulado/toxicidad , SARS-CoV-2RESUMEN
Auxin regulates a plethora of events during plant growth and development, acting in concert with other phytohormones. YUCCA genes encode flavin monooxygenases that function in tryptophan-dependent auxin biosynthesis. To understand the contribution of the YUCCA4 (YUC4) gene on auxin homeostasis, plant growth and interaction with abscisic acid (ABA) signaling, 35S::YUC4 seedlings were generated, which showed elongated hypocotyls with hyponastic leaves and changes in root system architecture that correlate with enhanced auxin responsive gene expression. Differential expression of PIN1, 2, 3 and 7 auxin transporters was detected in roots of YUC4 overexpressing seedlings compared to the wild-type: PIN1 was down-regulated whereas PIN2, PIN3 and PIN7 were up-regulated. Noteworthy, 35S::YUC4 lines showed enhanced sensitivity to ABA on seed germination and post-embryonic root growth, involving ABI4 transcription factor. The auxin reporter genes DR5::GUS, DR5::GFP and BA3::GUS further revealed that abscisic acid impairs auxin responses in 35S::YUC4 seedlings. Our results indicate that YUC4 overexpression influences several aspects of auxin homeostasis and reveal the critical roles of ABI4 during auxin-ABA interaction in germination and primary root growth.
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MAIN CONCLUSION: A MAPK module, of which MPK6 kinase is an important component, is involved in the coordination of the responses to Pi and Fe in the primary root meristem of Arabidopsis thaliana. Phosphate (Pi) deficiency induces determinate primary root growth in Arabidopsis through cessation of cell division in the meristem, which is linked to an increased iron (Fe) accumulation. Here, we show that Mitogen-Activated Protein Kinase6 (MPK6) has a role in Arabidopsis primary root growth under low Pi stress. MPK6 activity is induced in roots in response to low Pi, and such induction is enhanced by Fe supplementation, suggesting an MPK6 role in coordinating Pi/Fe balance in mediating root growth. The differentiation of the root meristem induced by low Pi levels correlates with altered expression of auxin-inducible genes and auxin transporter levels via MPK6. Our results indicate a critical role of the MPK6 kinase in coordinating meristem cell activity to Pi and Fe availability for proper primary root growth.
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Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Hierro/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Fosfatos/metabolismo , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Proteínas de Arabidopsis/genética , Transporte Biológico , División Celular , Genes Reporteros , Ácidos Indolacéticos/metabolismo , Meristema/enzimología , Meristema/genética , Meristema/crecimiento & desarrollo , Proteínas Quinasas Activadas por Mitógenos/genética , Reguladores del Crecimiento de las Plantas , Raíces de Plantas/enzimología , Raíces de Plantas/genética , Raíces de Plantas/crecimiento & desarrollo , Estrés FisiológicoRESUMEN
BACKGROUND: Seed germination is a crucial process in the plant life cycle when a dramatic variation of type and sugar content occurs just as the seed is hydrated. The production of hexose 6 phosphate is a key node in different pathways that are required for a successful germination. Hexokinase (HXK) is the only plant enzyme that phosphorylates glucose (Glc), so it is key to fueling several metabolic pathways depending on their substrate specificity, metabolite regulatory responses and subcellular localization. In maize, the HXK family is composed of nine genes, but only six of them (ZmHXK4-9) putatively encode catalytically active enzymes. Here, we cloned and functionally characterized putative catalytic enzymes to analyze their metabolic contribution during germination process. RESULTS: From the six HXKs analyzed here, only ZmHXK9 has minimal hexose phosphorylating activity even though enzymatic function of all isoforms (ZmHXK4-9) was confirmed using a yeast complementation approach. The kinetic parameters of recombinant proteins showed that ZmHXK4-7 have high catalytic efficiency for Glc, fructose (Fru) and mannose (Man), ZmHXK7 has a lower Km for ATP, and together with ZmHXK8 they have lower sensitivity to inhibition by ADP, G6P and N-acetylglucosamine than ZmHXK4-6 and ZmHXK9. Additionally, we demonstrated that ZmHXK4-6 and ZmHXK9 are located in the mitochondria and their location relies on the first 30 amino acids of the N-terminal domain. Otherwise, ZmHXK7-8 are constitutively located in the cytosol. HXK activity was detected in cytosolic and mitochondrial fractions and high Glc and Fru phosphorylating activities were found in imbibed embryos. CONCLUSIONS: Considering the biochemical characteristics, location and the expression of ZmHXK4 at onset of germination, we suggest that it is the main contributor to mitochondrial activity at early germination times, at 24 h other ZmHXKs also contribute to the total activity. While in the cytosol, ZmHXK7 could be responsible for the activity at the onset of germination, although later, ZmHXK8 also contributes to the total HXK activity. Our observations suggest that the HXKs may be redundant proteins with specific roles depending on carbon and ATP availability, metabolic needs, or sensor requirements. Further investigation is necessary to understand their specific or redundant physiological roles.
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Citosol/fisiología , Germinación/fisiología , Hexoquinasa/metabolismo , Semillas/fisiología , Zea mays/enzimología , Zea mays/fisiología , Citosol/enzimología , Citosol/metabolismo , Germinación/genética , Hexoquinasa/genética , Mitocondrias/enzimología , Mitocondrias/metabolismo , Semillas/enzimología , Semillas/metabolismo , Zea mays/metabolismoRESUMEN
Abstract Introduction: Alcohol consumption has a high prevalence in society and its chronic consumption is one of the main factors related to health condition in an individual, along with other aspects concerning lifestyle. Regarding the health-disease correlation, it is one of the main determinants of health, from an epidemiological point of view, and has been a traditional object of study from different perspectives and in diverse population groups. Objective: To compare consumption patterns and related variables in two different groups: patients in mental health units and workers in general. Materials and methods: Cross-sectional study in a population of 1 180 service workers and 304 patients in a mental health unit. Sociodemographic and work aspects, as well as pattern of alcohol consumption were analyzed for both populations. Results: Differences in both groups were observed regarding the number of consumers, quantity of consumption and type of beverages consumed, differences that disappear, in part, when comparing groups of workers of both population samples. Conclusions: Differences in the consumption pattern of both populations lead to different preventive-assistance strategies and to the need for implementing coordinated actions by specific programs among those affected.
Resumen Introducción. El consumo de alcohol tiene una elevada prevalencia en la sociedad y su consumo crónico es uno de los principales factores relacionados con el estado de salud de los individuos, junto a otros aspectos concernientes al estilo de vida. Debido a su importancia, este tema ha sido objeto tradicional de estudio desde diferentes perspectivas epidemiológicas y en diversos colectivos poblacionales. Objetivo. Realizar una comparativa de patrones de consumo y variables relacionadas en dos colectivos diferenciados: pacientes de unidades de salud mental y trabajadores en general. Materiales y métodos. Estudio transversal realizado en una población de 1 180 trabajadores del sector servicios de la administración pública y 304 pacientes de una unidad de salud mental. Se analizaron variables sociodemográficas y laborales y el patrón de consumo de alcohol. Resultados. Existen diferencias en ambos colectivos en cuanto al número de consumidores, cantidad de consumo y tipo de bebidas consumidas, diferencias que desaparecen en parte cuando se comparan colectivos de trabajadores de ambas muestras poblacionales. Conclusiones. Las diferencias en el patrón de consumo de ambas poblaciones orientan hacia actuaciones preventivo-asistenciales distintas en ambos grupos y hacia una necesidad de implementar actuaciones coordinadas entre todos los afectados mediante programas específicos.
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INTRODUCCIÓN: El riesgo cardiovascular es una de las principales pre ocupaciones de los servicios de salud laboral. MATERIAL Y MÉTODOS: Se realiza un estudio retrospectivo y transversal en 2944 varones conductores profesionales (487 taxistas, 908 con ductores de autobús y 1549 conductores de camión) y se valoran diferentes variables relacionadas con riesgo cardiovascular. RESULTADOS: El 27,4% de los conductores son obesos, el 25% hiper tensos, un 48% presentan valores elevados de colesterol y un 29,5% triglicéridos altos, mientras la hiperglucemia aparece en el 6,5%. Un 15,8% cumplen los criterios de síndrome metabólico, un 2,6% presentan valores elevados en la escala REGICOR y un 7,9% en la escala SCORE. El riesgo elevado de padecer diabetes tipo 2 con la escala Findrisk aparece en el 13% de los conductores. CONCLUSIONES: Globalmente, el colectivo de taxistas es el que presenta peores resultados en todas las variables relacionadas con riesgo cardiovascular.
INTRODUCTION: Cardiovascular risk is one of the main concerns of occupational health services. MATERIAL AND METHODS: A retrospective and cross-sectional study was conducted in 2944 professional male drivers (487 taxi drivers, 908 bus drivers and 1549 truck drivers) and different variables related to cardiovascular risk were assessed. RESULTS: 27.4% of the drivers are obese, 25% hypertensive, 48% have high cholesterol and 29.5% high triglycerides, while hyperglycemia appears in 6.5%. 15.8% met the metabolic syndrome criteria, 2.6% presented high values on the REGICOR scale and 7.9% on the SCORE scale. The high risk of type 2 diabetes with the Findrisk scale appears in 13% of drivers. CONCLUSIONS: Globally the group of taxi drivers is the one that pres ents worse results in all the variables related to cardiovascular risk.
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Humanos , Masculino , Adulto , Conducción de Automóvil , Enfermedades Cardiovasculares/etiología , Medición de Riesgo/métodos , España , Transportes , Triglicéridos/análisis , Enfermedades Cardiovasculares/epidemiología , Colesterol/análisis , Estudios Transversales , Análisis Multivariante , Estudios Retrospectivos , Factores de Riesgo , Diabetes Mellitus Tipo 2 , Estilo de Vida Saludable , Hiperglucemia , Hipertensión/epidemiología , Obesidad/epidemiologíaRESUMEN
KEY MESSAGE: The function and components of L-glutamate signaling pathways in plants have just begun to be elucidated. Here, using a combination of genetic and biochemical strategies, we demonstrated that a MAPK module is involved in the control of root developmental responses to this amino acid. Root system architecture plays an essential role in plant adaptation to biotic and abiotic factors via adjusting signal transduction and gene expression. L-Glutamate (L-Glu), an amino acid with neurotransmitter functions in animals, inhibits root growth, but the underlying genetic mechanisms are poorly understood. Through a combination of genetic analysis, in-gel kinase assays, detailed cell elongation and division measurements and confocal analysis of expression of auxin, quiescent center and stem cell niche related genes, the critical roles of L-Glu in primary root growth acting through the mitogen-activated protein kinase 6 (MPK6) and the dual specificity serine-threonine-tyrosine phosphatase MKP1 could be revealed. In-gel phosphorylation assays revealed a rapid and dose-dependent induction of MPK6 and MPK3 activities in wild-type Arabidopsis seedlings in response to L-Glu. Mutations in MPK6 or MKP1 reduced or increased root cell division and elongation in response to L-Glu, possibly modulating auxin transport and/or response, but in a PLETHORA1 and 2 independent manner. Our data highlight MPK6 and MKP1 as components of an L-Glu pathway linking the auxin response, and cell division for primary root growth.
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Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Arabidopsis/fisiología , Ácido Glutámico/farmacología , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Raíces de Plantas/enzimología , Proteínas Tirosina Fosfatasas/metabolismo , Arabidopsis/crecimiento & desarrollo , Proteínas de Arabidopsis/biosíntesis , Proliferación Celular/efectos de los fármacos , Inducción Enzimática/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Ácidos Indolacéticos/farmacología , Proteínas de Transporte de Membrana/metabolismo , Meristema/efectos de los fármacos , Meristema/enzimología , Proteínas Quinasas Activadas por Mitógenos/biosíntesis , Mutación/genética , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Proteínas Tirosina Fosfatasas/biosíntesis , Factores de Transcripción/metabolismoRESUMEN
In Spanish, the concepts of discapacidad (disability leave) and incapacidad (sick leave) jointly refer to the impairment of a person due to injuries, diseases or deficiencies that limit their activity in a social, personal or occupational field. However, this common link does not imply that both concepts are the same. Statistical data from INE (Instituto Nacional de Estadística: Statistic National Institute) show that Spain had in 2015 3.85 million persons with a disability (59.8% were women). Statistical data from 2015 from INSS (Instituto Nacional de Seguridad Social: Social Security National Institute) show high levels in the number of processes and in workers affected by temporary sick leave, with social costs to the social security system. Both concepts have been updated: about disability leave, Law 39/2006 adjusted terminology by avoiding the use of concepts with discriminating or pejorative connotation. Regarding sick leave, the Ley General de Seguridad Social (General Social Security Law)has been amended and came into effect in January, 2016. It is necessary to know and distinguish these aspects for a better administrative management, and a more oriented information to the affected patient.
Los conceptos de discapacidad e incapacidad hacen referencia conjuntamente al menoscabo de una persona por lesiones, enfermedades o deficiencias que limitan su actividad en el ámbito social, personal o laboral. Pero este nexo común no implica equiparación entre ambos. Datos estadísticos del 2015 del Instituto Nacional de Estadística (INE) muestran en España 3.85 millones de personas con alguna discapacidad, el 59.8% mujeres. Datos estadísticos del 2015 del Instituto Nacional de la Seguridad Social (INSS), tanto en número de procesos, como de trabajadores afectados por incapacidad muestran cifras elevadas, con costos sociales en prestaciones a cargo de la seguridad social. Ambos conceptos han sido objeto de actualización legislativa: en discapacidad, la Ley 39/2006 ajusta la terminología y evita el uso de conceptos con connotación peyorativa o discriminativa. En incapacidad, la Ley General de la Seguridad Social ha sido modificada y ha entrado en vigor en enero de 2016. Es necesario conocer y diferenciar estos aspectos para una mejor gestión administrativa, e información más orientada al paciente afectado.
Asunto(s)
Evaluación de la Discapacidad , Ausencia por Enfermedad/legislación & jurisprudencia , Femenino , Humanos , Masculino , Ausencia por Enfermedad/economía , España , Terminología como AsuntoRESUMEN
KEY MESSAGE: Arabidopsis med12 and med13 mutants exhibit shoot and root phenotypes related to an altered auxin homeostasis. Sucrose supplementation reactivates both cell division and elongation in primary roots as well as auxin-responsive and stem cell niche gene expression in these mutants. An analysis of primary root growth of WT, med12, aux1-7 and med12 aux1 single and double mutants in response to sucrose and/or N-1-naphthylphthalamic acid (NPA) placed MED12 upstream of auxin transport for the sugar modulation of root growth. The MEDIATOR (MED) complex plays diverse functions in plant development, hormone signaling and biotic and abiotic stress tolerance through coordination of transcription. Here, we performed genetic, developmental, molecular and pharmacological analyses to characterize the role of MED12 and MED13 on the configuration of root architecture and its relationship with auxin and sugar responses. Arabidopsis med12 and med13 single mutants exhibit shoot and root phenotypes consistent with altered auxin homeostasis including altered primary root growth, lateral root development, and root hair elongation. MED12 and MED13 were required for activation of cell division and elongation in primary roots, as well as auxin-responsive and stem cell niche gene expression. Remarkably, most of these mutant phenotypes were rescued by supplying sucrose to the growth medium. The growth response of primary roots of WT, med12, aux1-7 and med12 aux1 single and double mutants to sucrose and application of auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) revealed the correlation of med12 phenotype with the activity of the auxin intake permease and suggests that MED12 acts upstream of AUX1 in the root growth response to sugar. These data provide compelling evidence that MEDIATOR links sugar sensing to auxin transport and distribution during root morphogenesis.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Carbohidratos/farmacología , Genes de Plantas , Ácidos Indolacéticos/farmacología , Raíces de Plantas/genética , Raíces de Plantas/fisiología , Proteínas Represoras/genética , Arabidopsis/efectos de los fármacos , Arabidopsis/fisiología , Proteínas de Arabidopsis/metabolismo , División Celular/efectos de los fármacos , División Celular/genética , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Proteínas de Transporte de Membrana/genética , Proteínas de Transporte de Membrana/metabolismo , Mutación/genética , Fenotipo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Raíces de Plantas/citología , Raíces de Plantas/crecimiento & desarrollo , Proteínas Represoras/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Nicho de Células Madre/efectos de los fármacos , Sacarosa/farmacologíaRESUMEN
BACKGROUND: The Finnish Diabetes Risk Score (FINDRISC) questionnaire has been used to assess the risk of type 2 diabetes and metabolic syndrome. The objetive was to assess the relationship between different scales related to cardiovascular risk and FINDRISC questionnaire. METHODS: Values of different anthropometric and clinical parameters (body mass index, waist circumference, waist to height ratio, blood pressure), analytical parameters (lipid profile, blood glucose) and scales related to cardiovascular risk (atherogenic index, metabolic syndrome, REGICOR, SCORE, heart age and vascular age) were determined on the basis of the value of the FINDRISC questionnaire. RESULTS: All analyzed parameters related to cardiovascular risk were getting worse at the same time that the value of the FINDRISC questionnaire increased. CONCLUSIONS: There is a close relationship between FINDRISC questionnaire values and those obtained in the different parameters by which cardiovascular risk was measured directly or indirectly.
Introducción: el Finnish Diabetes Risk Score (test de FINDRISC) se ha empleado para valorar el riesgo de padecer diabetes tipo 2 y síndrome metabólico. El objetivo consistió en valorar la relación entre diferentes escalas relacionadas con el riesgo cardiovascular y el test de FINDRISC. Métodos: se determinaron los valores de difentes parámetros antropométricos, clínicos (índice de masa corporal, perímetro de cintura, índice cintura altura, tensión arterial), analíticos (perfil lipídico y glucemia) y escalas relacionadas con riesgo cardiovascular (indices aterogénicos, síndrome metabólico, REGICOR, SCORE, edad del corazón y edad vascular) según el valor del test de FINDRISC. Resultados: todos los parámetros relacionados con riesgo cardiovascular analizados fueron empeorando a medida que aumentaba el valor del test de FINDRISC. Conclusión: existe una estrecha relación entre los valores del test de FINDRISC y los obtenidos en los diferentes parámetros y escalas que valoran de forma directa o indirecta el riesgo cardiovascular.
Asunto(s)
Enfermedades Cardiovasculares/diagnóstico , Diabetes Mellitus Tipo 2/diagnóstico , Indicadores de Salud , Adulto , Enfermedades Cardiovasculares/etiología , Estudios Transversales , Diabetes Mellitus Tipo 2/etiología , Femenino , Humanos , Masculino , Región Mediterránea , Persona de Mediana Edad , Medición de Riesgo , Factores de Riesgo , EspañaRESUMEN
INTRODUCTION: The impaired fasting glucose (IFG) is an intermediate clinical situation between normal glucose and type 2 diabetes, it is known that 25% of people with IFG progress to type 2 diabetes. METHODS: Descriptive study in 60,798 Spanish workers. The IFG was determined using the American Diabetes Association (ADA) criteria: 100-125 mg/dl and the World Health Organization (WHO) criteria: 110-125 mg/dl. The influence of sociodemographic variables (age, sex, social class, studies class) and healthy habits (tobacco, alcohol consumption, physical activity, feeding) were evaluated in the IFG prevalence. RESULTS: The IFG prevalence was 3.3% (1.8% in women and 4.5% in men) using the WHO criteria and 11.8% (7% in women and 15.4% in men) using the ADA criteria. The IFG is more common in men and increases with age. People with low socioeconomic status (social classes IV and V, blue collar and primary studies) and poor health habits (smokers, heavy alcohol consumption and low physical activity) have a higher prevalence. CONCLUSIONS: The sociodemographic variables and the healthy habits, except consumption of fruits and vegetables, influence the prevalence of IFG.