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Basketball players need to frequently engage in various physical movements during the game, which puts a certain burden on their bodies and can easily lead to various sports injuries. Therefore, it is crucial to prevent sports injuries in basketball teaching. This paper also studies basketball motion track capture. Basketball motion capture preserves the motion posture information of the target person in three-dimensional space. Because the motion capture system based on machine vision often encounters problems such as occlusion or self occlusion in the application scene, human motion capture is still a challenging problem in the current research field. This article designs a multi perspective human motion trajectory capture algorithm framework, which uses a two-dimensional human motion pose estimation algorithm based on deep learning to estimate the position distribution of human joint points on the two-dimensional image from each perspective. By combining the knowledge of camera poses from multiple perspectives, the three-dimensional spatial distribution of joint points is transformed, and the final evaluation result of the target human 3D pose is obtained. This article applies the research results of neural networks and IoT devices to basketball motion capture methods, further developing basketball motion capture systems.
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Objective@#To explore the mechanisms of oxidative stress-induced damage to TM4 Sertoli cells in the mouse using metabolomics techniques based on gas chromatography-mass spectrometry (GC-MS).@*METHODS@#We established the model of oxidative stress-induced damage to mouse TM4 Sertoli cells by treatment with H₂O₂. Then, we detected the survival rate and apoptosis rate of the TM4 cells by MTT and flow cytometry respectively, measured the concentration of ROS in the TM4 cells with the DCFH-DA fluorescent probe, and determined the levels of endogenous metabolites in the TM4 cells by GC-MS after H₂O₂ intervention.@*RESULTS@#After 2 hours of treatment with H₂O₂ at 600 μmol/L, the survival rate of the TM4 cells was reduced to about 50%, and the total apoptosis rates in the low- (100 μmol/L), medium- (300 μmol/L), and high-dose (600 μmol/L) groups were (19.45 ± 0.53), (20.12 ± 0.58), and (37.13 ± 0.35)%, respectively, increased in a dose-dependent manner as compared with (10.28 ± 0.35)% in the blank control (P 1, P <0.05).@*CONCLUSIONS@#Oxidative stress-induced damage and apoptosis of TM4 Sertoli cells are closely associated with the metabolism of amino acid, glucose, and energy in the cells.
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Animales , Masculino , Ratones , Aminoácidos , Metabolismo , Apoptosis , Supervivencia Celular , Relación Dosis-Respuesta a Droga , Metabolismo Energético , Cromatografía de Gases y Espectrometría de Masas , Glucosa , Metabolismo , Peróxido de Hidrógeno , Farmacología , Metabolómica , Estrés Oxidativo , Especies Reactivas de Oxígeno , Metabolismo , Células de Sertoli , Metabolismo , Factores de TiempoRESUMEN
The association between ambient temperature and acute exacerbation of chronic bronchitis (AECB) was still unknown. Therefore, we performed an epidemiological study in a large hospital of Shanghai to explore the relationship about temperature and outpatient visit for AECB. We adopted a quasi-Poisson generalized additive models and distributed lag nonlinear models to estimate the accumulative effects of temperature on AECB across multiple days. We found significant non-linear effects of cold temperature on hospital visits for AECB, and the potential effect of cold temperature might last more than 2 weeks. The relative risks of extreme cold (first percentiles of temperature throughout the study period) and cold (10th percentile of temperature) temperature over lags 0-14 d were 2.98 [95% confidence intervals (CI): 1.77, 5.04] and 1.63 (95% CI: 1.21, 2.19), compared with the 25th percentile of temperature. However, we found no positive association between hospital visits and hot weather. This study showed that exposure to both extreme cold and cold temperatures were associated with increased outpatient visits for AECB in a large hospital of Shanghai.
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Adolescente , Adulto , Anciano , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Adulto Joven , Contaminantes Atmosféricos , Atención Ambulatoria , Bronquitis Crónica , Epidemiología , Patología , China , Epidemiología , Frío , Tiempo (Meteorología)RESUMEN
<p><b>OBJECTIVE</b>To investigate the short-term association between outdoor air pollution and outpatient visits for acute bronchitis, which is a rare subject of research in the mainland of China.</p><p><b>METHODS</b>A time-series analysis was conducted to examine the association of outdoor air pollutants with hospital outpatient visits in Shanghai by using two-year daily data (2010-2011).</p><p><b>RESULTS</b>Outdoor air pollution was found to be associated with an increased risk of outpatient visits for acute bronchitis in Shanghai. The effect estimates of air pollutants varied with the lag structures of the concentrations of the pollutants. For lag06, a 10 μg/m(3) increase in the concentrations of PM10, SO(2), and NO(2) corresponded to 0.94% (95% CI: 0.83%, 1.05%), 11.12% (95% CI: 10.76%, 11.48%), and 4.84% (95% CI: 4.49%, 5.18%) increases in hospital visits for acute bronchitis, respectively. These associations appeared to be stronger in females (P<0.05). Between-age differences were significant for SO(2) (P<0.05), and between-season differences were also significant for SO(2) (P<0.05).</p><p><b>CONCLUSION</b>Our analyses have provided the first evidence that the current air pollution level in China has an effect on acute bronchitis and that the rationale for further limiting air pollution levels in Shanghai should be strengthened.</p>