RESUMEN
Bivalve mollusks as typical osmoconformers are unable to maintain a constant level of internal osmolarity in conditions of salinity stress. Adaptation to fluctuations of environmental salinity is achieved through cellular osmoregulatory responses, which are accompanied with a substantial shift in functional state of cells. In the present work we investigated the effect of hypersalinity stress on hemolymph cellular composition and morphology of the ark clam (Anadara kagoshimensis) hemocytes. Ark clams were subjected to a gradual increase of environmental salinity from 18 to 35 and 45 and maintained at those conditions for two days. Exposure to hypersalinity 35 induced changes in erythrocyte morphology and led to a decrease of their diameter. At salinity 45 a substantial increase of hemocyte average diameter was observed, whereas the shape of cells did not change (18). Hyperosmotic stress was not associated with changes in hemocyte viability as well as changes in hemolymph cellular composition. The results of the present work demonstrate high tolerance of A. kagoshimensis to short-time exposure to hypersalinic conditions.
Asunto(s)
Bivalvos , Scapharca , Animales , Hemocitos , Hemolinfa , SalinidadRESUMEN
Bivalve mollusks are frequently subjected to fluctuations of dissolved oxygen concentration in the environment which can represent a significant threat to bivalve antioxidant status. In this work the effects of hypoxia on hemocyte reactive oxygen species (ROS) production and level of mitochondrial potential as well as the activity and expression level of catalase (CAT) and superoxide dismutase (SOD) in gills of Crassostrea gigas were investigated after 24 h and 72 h exposure. 24 h hypoxia promoted an increase of mitochondrial membrane potential in agranulocytes and induced ROS accumulation in granulocytes. 72 h exposure substantially decreased hemocyte mitochondrial potential and intracellular ROS level in all hemocyte types. No significant changes in the activity of CAT in gills were observed following both 24 h and 72 h exposure periods compared to control. SOD activity in gills decreased after 72 h exposure to hypoxia but did not change under 24 h hypoxia. Significant up-regulation of SOD gene and no changes in expression level of CAT were observed in all experimental groups. The results indicate an overall shift in antioxidant status in gills and hemocytes of the Pacific oyster that may act as compensatory mechanisms to maintain redox homeostasis after a short-term (24 h) exposure and represent the occurrence of oxidative stress conditions at the end of 72 h hypoxia.
Asunto(s)
Crassostrea , Animales , Antioxidantes , Crassostrea/genética , Branquias , Hemocitos , HipoxiaRESUMEN
Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a role in the regulation of osmotic responses in fish red blood cells is still not fully understood. We found that changes in hemoglobin conformation influence the pattern of the regulatory volume decrease in Carassius carassius red blood cells. In oxygenated cells (96.4 ± 3.7% oxygenated hemoglobin), the volume recovery was completed within 125 min. Deoxygenation of hemoglobin (96.5 ± 2.7% of deoxygenated hemoglobin) inhibited the volume decrease in hyposmotically swollen red blood cells. Reoxygenation restored regulatory volume decrease in cells within 5 min. Induced methemoglobinemia (48.4 ± 1.8% of methemoglobin and 41.3 ± 2.3% of deoxygenated hemoglobin) blocked the process of volume recovery and significantly decreased osmotic stability of red blood cells.
Asunto(s)
Carpas , Tamaño de la Célula , Eritrocitos/citología , Hemoglobinas/química , Metahemoglobinemia , Animales , Presión Osmótica , Oxígeno/sangreRESUMEN
The circulating hemocytes of cultivated marine mussel (Mytilus galloprovincialis) were investigated using light microscopy and flow cytometry. In mussels two cell types, granulocytes and agranulocytes, were identified based on the existence of two subpopulations of cells differing by size and granularity level on light-scattered plots. Light microscopic observation confirmed the presence of cells with cytoplasmic granules and cells without granulation in hemolymph of mussels. The main type of cells in hemolymph were agranular cells amounting 78.4⯱â¯8.9% in mussels. Flow cytometry showed that the agranular hemocytes of the mollusks produce significantly less reactive oxygen species compared to granulocytes. Mussel were exposed for 24â¯h of hypoxia and immune functions including hemocyte mortality, proliferation and reactive oxygen species (ROS) production were analysed using flow cytometric methods. Granulocyte number was higher at low oxygen concentration than that at normoxia; agranulocytes number decreased, in contrast. The ROS production after hypoxic treatment was decreased compared to normoxia level. No significant changes in hemocyte mortality and proliferation were observed.