RESUMEN
Parameters of hepatic free radical oxidation and catalytic properties of NADP-isocitrate dehydrogenase (EC 1.1.1.42; NADP-IDH) have been investigated in normal rats and under conditions of toxic hepatitis. Development of hepatitis was accompanied by the increase of conjugated dienes, malondialdehyde and some chemiluminescence parameters. Toxic hepatitis was also accompanied by the increase of liver NADP-IDH. Homogenous preparations of liver NADP-IDH from normal and toxic rats exhibited different sensitivity to effectors studied (Fe2+, Ca2+, H2O2, reduced glutatione).
Asunto(s)
Intoxicación por Tetracloruro de Carbono/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Isocitrato Deshidrogenasa/metabolismo , Hígado/metabolismo , Animales , Intoxicación por Tetracloruro de Carbono/enzimología , Enfermedad Hepática Inducida por Sustancias y Drogas/enzimología , Activación Enzimática , Radicales Libres , Isocitrato Deshidrogenasa/antagonistas & inhibidores , Cinética , Hígado/enzimología , Masculino , Oxidación-Reducción , RatasRESUMEN
We observed intensification of free radical oxidation, decrease in activity, and changes in catalytic properties of aconitate hydratase in the liver of rats with toxic hepatitis. The total yield and maximum flash intensity of biochemiluminescence increased by 2.2 and 1.7 times, respectively. Differences were revealed in the regulation of aconitate hydratase activity with Fe(2+), Ca(2+), H(2)O(2), and oxidized and reduced glutathione in the liver of rats with toxic hepatitis and control animals.