RESUMEN
The envelope glycoprotein complex (Env) of human immunodeficiency virus-1 (HIV-1) can induce apoptosis by a cornucopia of distinct mechanisms. A soluble Env derivative, gp120, can kill cells through signals that are transmitted by chemokine receptors such as CXCR4. Cell surface-bound Env (gp120/gp41), as present on the plasma membrane of HIV-1-infected cells, can kill uninfected bystander cells expressing CD4 and CXCR4 (or similar chemokine receptors, depending on the Env variant) by at least three different mechanisms. First, a transient interaction involving the exchange of lipids between the two interacting cells ('the kiss of death') may lead to the selective death of single CD4-expressing target cells. Second, fusion of the interacting cells may lead to the formation of syncytia which then succumb to apoptosis in a complex pathway involving the activation of several kinases (cyclin-dependent kinase-1, Cdk1; checkpoint kinase-2, Chk2; mammalian target of rapamycin, mTOR; p38 mitogen-activated protein kinase, p38 MAPK; inhibitor of NF-kappaB kinase, IKK), as well as the activation of several transcription factors (NF-kappaB, p53), finally resulting in the activation of the mitochondrial pathway of apoptosis. Third, if the Env-expressing cell is at an early stage of imminent apoptosis, its fusion with a CD4-expressing target cell can precipitate the death of both cells, through a process that may be considered as contagious apoptosis and which does not involve Cdk1, mTOR, p38 nor p53, yet does involve mitochondria. Activation of some of the above- mentioned lethal signal transducers have been detected in patients' tissues, suggesting that HIV-1 may indeed trigger apoptosis through molecules whose implication in Env-induced killing has initially been discovered in vitro.
Asunto(s)
Apoptosis , Proteína gp120 de Envoltorio del VIH/farmacología , VIH-1 , Receptores de Quimiocina/efectos de los fármacos , Animales , Antígenos CD4/efectos de los fármacos , Células Cultivadas , Productos del Gen vpr/farmacología , Células Gigantes/efectos de los fármacos , Células Gigantes/metabolismo , Proteína gp120 de Envoltorio del VIH/fisiología , VIH-1/patogenicidad , Humanos , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Receptores CCR5/efectos de los fármacos , Receptores CXCR4/efectos de los fármacos , Receptores de Quimiocina/metabolismo , Transducción de Señal , Productos del Gen vpr del Virus de la Inmunodeficiencia HumanaRESUMEN
The third reading frame of the envelope gene from HIV-1 codes for a protein homologous to the human selenoprotein glutathione peroxidase (GPX). Cells stably or transiently transfected with a HIV-1 GPX construct are protected against the loss of the mitochondrial transmembrane potential and subsequent cell death induced by exogenous reactive oxygen species (ROS) as well as mitochondrion-generated ROS. However, HIV-1 GPX does not confer a general apoptosis resistance, because HIV-1 GPX-transfected cells were not protected against cell death induced by staurosporine or oligomycin. The inhibition of cell death induced by the ROS donor tert-butylhydroperoxide was also observed in cells depleted from endogenous glutathione (GSH), suggesting that GSH is not the sole electron acceptor for HIV-1 GPX. Clinical HIV-1 isolates from long-term non-progressors (untreated patients with diagnosed HIV-1 infection for > 10 years, with CD4 T cell count of > 500 cells/mm3) mostly possess an intact GPX gene (with only 18% of loss-of-function mutations), while HIV-1 isolates from patients developing AIDS contain non-functional GPX mutants in 9 out of 17 cases (53%). Altogether, these data suggest that HIV-1 GPX possesses a cytoprotective, pathophysiologically relevant function.
Asunto(s)
Apoptosis/fisiología , Glutatión Peroxidasa/metabolismo , VIH-1/enzimología , Secuencia de Aminoácidos , Secuencia Conservada , Glutatión/metabolismo , Glutatión Peroxidasa/genética , VIH-1/genética , VIH-1/metabolismo , Humanos , Mitocondrias/metabolismo , Datos de Secuencia Molecular , Especies Reactivas de Oxígeno/metabolismo , TransfecciónRESUMEN
Previous biochemical studies suggested that HIV-1-encoded Vpr may kill cells through an effect on the adenine nucleotide translocase (ANT), thereby causing mitochondrial membrane permeabilization (MMP). Here, we show that Vpr fails to activate caspases in conditions in which it induces cell killing. The knock-out of essential caspase-activators (Apaf-1 or caspase-9) or the knock-out of a mitochondrial caspase-independent death effector (AIF) does not abolish Vpr-mediated killing. In contrast, the cytotoxic effects of Vpr are reduced by transfection-enforced overexpression of two MMP-inhibitors, namely the endogenous protein Bcl-2 or the cytomegalovirus-encoded ANT-targeted protein vMIA. Vpr, which can elicit MMP through a direct effect on mitochondria, and HIV-1-Env, which causes MMP through an indirect pathway, exhibit additive (but not synergic) cytotoxic effects. In conclusion, it appears that Vpr induces apoptosis through a caspase-independent mitochondrial pathway.
Asunto(s)
Apoptosis/fisiología , Productos del Gen vpr/metabolismo , VIH-1/metabolismo , Mitocondrias/metabolismo , Proteínas Virales , Factor Inductor de la Apoptosis , Caspasas/metabolismo , Línea Celular , Citomegalovirus/metabolismo , Flavoproteínas/fisiología , Productos del Gen env/metabolismo , Humanos , Proteínas Inmediatas-Precoces/metabolismo , Metaloproteinasas de la Matriz/metabolismo , Proteínas de la Membrana/fisiología , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Células Madre , Productos del Gen vpr del Virus de la Inmunodeficiencia HumanaRESUMEN
In lymphocytes, glucocorticoids (GC)- and interleukin-4-signaling pathways are known to interact, as evidenced by inhibition of IL-4-mediated proliferation by dexamethasone or suppression of GC-induced apoptosis by IL-4. In this study, we characterized the molecular basis for this reciprocal interference. We report that, in murine CTLL-2 cells, IL-4 inhibits GC-induced MMTV (mouse mammary tumor virus) promoter transactivation, and that GC suppress IL-4-induced transactivation of a STAT6 (signal transducers and activators of transcription 6)-responsive promoter without affecting IL-4-stimulated STAT6 DNA-binding. Moreover, we evidenced a physical association between GC receptor and STAT6, which proved to be functionally relevant, since STAT6 overexpression increased the IL-4 inhibitory effect on GC-induced MMTV transactivation.
Asunto(s)
Dexametasona/farmacología , Interleucina-4/farmacología , Proteínas de la Leche , Receptores de Glucocorticoides/fisiología , Linfocitos T Citotóxicos/fisiología , Transactivadores/metabolismo , Animales , Apoptosis/efectos de los fármacos , Apoptosis/fisiología , Línea Celular , Proteínas de Unión al ADN/metabolismo , Humanos , Virus del Tumor Mamario del Ratón/genética , Ratones , Regiones Promotoras Genéticas , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacología , Factor de Transcripción STAT5 , Factor de Transcripción STAT6 , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Linfocitos T Citotóxicos/citología , Linfocitos T Citotóxicos/efectos de los fármacos , Transactivadores/genética , Activación Transcripcional/efectos de los fármacos , Activación Transcripcional/fisiología , TransfecciónRESUMEN
Synthetic lipids were examined for their ability to mimic or to antagonize lipopolysaccharide (LPS) action in murine B-lymphocytes. Several recognized effects of LPS were analyzed: prevention of spontaneous apoptosis, expression of alkaline phosphatase (ALP) and stimulation of proliferation. Three synthetic lipids were used for that purpose: a lipopeptide (compound MTPP) which carries non-hydroxylated fatty acids, and is thus unrelated to LPS, and two glycolipids with hydroxylated fatty acids (compounds D2 and PPDm2-B), structurally related to the lipid A region of enterobacterial and Rhodopseudomonas LPS, respectively. We found that the ability of these lipids to induce LPS-like responses was not correlated with their structural analogy with LPS. Thus, the lipopeptide, MTPP, mimicked LPS in the three activities, whereas the glycolipid, D2, did not. In contrast, the ability of synthetic lipids to block LPS effects was correlated with their structural analogy with LPS. We thus observed that compound D2 selectively blocked LPS-induced ALP expression and that PPDm2-B selectively inhibited LPS-induced prevention of apoptosis. These synthetic lipids could therefore be useful for studying the LPS-mediated signals involved in B-cell activation and apoptosis.
Asunto(s)
Fosfatasa Alcalina/efectos de los fármacos , Apoptosis/efectos de los fármacos , Linfocitos B/efectos de los fármacos , Lípidos/farmacología , Fosfatasa Alcalina/biosíntesis , Animales , Linfocitos B/citología , Linfocitos B/enzimología , División Celular/efectos de los fármacos , ADN/efectos de los fármacos , ADN/metabolismo , Femenino , Citometría de Flujo , Lípidos/síntesis química , Lipopolisacáridos/química , Lipopolisacáridos/farmacología , Ratones , Ratones Endogámicos C57BLRESUMEN
Cycloheximide (CHX) is known to stimulate or to prevent apoptosis, according to the cell type used. We found that CHX, in a dose-dependent way, exerted the two opposite effects in B lymphocytes. CHXhigh (2.5 microg/mL) inhibited protein synthesis (>90%) and greatly increased B cell apoptosis but failed to prevent apoptosis induction by dexamethasone (DEX) or dibutyryl-cAMP (dbcAMP), which is in opposition with CHX activity in thymocytes. On the contrary, CHXlow (0.05 microg/mL), modestly inhibited protein synthesis (<15%) and reduced spontaneous as well as drug-induced apoptosis and further augmented the protection conferred by interleukin-4 or lipopolysaccharide. To examine the role of caspases in CHX effects, we used the broad spectrum peptide caspase inhibitor Z-VAD-fmk: it totally abrogated spontaneous as well as drug- and CHXhigh-induced cell death. Apoptosis was associated with CPP32/caspase-3 activation, since cleavage of CPP32/caspase-3 and caspase-3 activity were increased by DEX, dbcAMP as well as by CHXhigh treatment. Meanwhile, caspase-3 activity was reduced by CHXlow treatment. Therefore, CHX exerts opposite effects on B lymphocyte apoptosis which are associated with a dual action on caspase-3 activation.
Asunto(s)
Apoptosis , Linfocitos B/efectos de los fármacos , Caspasas/metabolismo , Cicloheximida/farmacología , Animales , Linfocitos B/enzimología , Linfocitos B/patología , Caspasa 3 , Caspasas/fisiología , División Celular/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Femenino , Ratones , Ratones Endogámicos C57BL , Biosíntesis de Péptidos/efectos de los fármacos , Inhibidores de la Síntesis de la Proteína/farmacologíaRESUMEN
The susceptibility to induction of apoptosis by the synthetic glucocorticoid, dexamethasone (Dex), was analysed at different stages of B cell maturation. Cells of the 70Z/3 pre-B cell line, expressing cytoplasmic mu chains, and LPS-stimulated 70Z/3 cells, expressing surface IgM, were used as a model of differentiation of pre-B cells into immature B cells. Cell proliferation and cell cycle progression were similarly inhibited by Dex (100 nM) in both naive 70Z/3 pre-B cells and in LPS-stimulated 70Z/3 cells. In contrast, Dex failed to affect apoptosis of naive 70Z/3 cells while it increased that of LPS-stimulated 70Z/3 cells. Splenic mature B lymphocytes were highly susceptible to Dex-induced apoptosis since subphysiological doses (5 nM) increased the frequency of apoptotic cells to more than 80%. On the other hand, the treatment of B lymphocytes with LPS, which led to proliferation and differentiation into immunoblasts, decreased the susceptibility to Dex-induced apoptosis. These effects were mediated by the glucocorticoid receptor since they were abrogated by the RU 486 antagonist. The response of B cells to glucocorticoids is thus dependent on their stage of differentiation.
Asunto(s)
Apoptosis/efectos de los fármacos , Linfocitos B/efectos de los fármacos , Dexametasona/farmacología , Glucocorticoides/farmacología , Animales , Diferenciación Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Línea Celular , Femenino , Citometría de Flujo , Lipopolisacáridos , Ratones , Ratones Endogámicos C57BL , Bazo/citología , Bazo/efectos de los fármacosRESUMEN
We have investigated the influence of age on B-cell responsiveness. The present study showed that the B-cell mitogen, lipopolysaccharide (LPS), similarly stimulated the proliferation of purified B lymphocytes obtained from either young mice (3 months) or old mice (24 months). In contrast, expression of the differentiation marker, alkaline phosphatase (ALP), was about fourfold higher in young mice than in older mice upon stimulation with LPS or with dextran sulfate (DXS) and interleukin-5 (IL-5). The occurrence of apoptosis during aging was then studied: unexpectedly, spontaneous cell death was double in B lymphocytes from young mice compared to older animals. Stimulation with DXS with or without IL-5 rescued B lymphocytes from cell death in young mice but protection decreased with aging, and no longer occurred in 24-month-old mice B cells. Meanwhile, the protective activity conferred by IL-4 was maintained at similar levels throughout aging. However, B cells from old mice were more responsive to apoptosis induction with cycloheximide, dibutyryl cAMP and dexamethasone. Together, the present results indicate an age-associated alteration in apoptosis and activation of B lymphocytes which could contribute to the age-related decline of the immune response.
Asunto(s)
Envejecimiento/inmunología , Apoptosis/inmunología , Linfocitos B/inmunología , Fosfatasa Alcalina/metabolismo , Animales , Apoptosis/efectos de los fármacos , División Celular/inmunología , Femenino , Ratones , Ratones EndogámicosRESUMEN
The role of caspases in B lymphocyte cell death was investigated by using two broad spectrum inhibitors of the caspase family, Z-Asp-cmk and Z-VAD-fmk. They totally prevented spontaneous and drug-induced apoptosis and inhibited the CPP32/caspase-3-like activity exhibited by apoptotic cells. However, the suppression of apoptosis was not associated with a long-term increase of cell survival, but conversely, with a switch from apoptotic death to the necrotic form. These results strongly suggest that apoptosis and necrosis share common initiation pathways, the final issue being determined by the presence of an active caspase.