RESUMEN
The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis.
Asunto(s)
Benzoilarginina-2-Naftilamida , Enfermedades Periodontales/diagnóstico , Porphyromonas gingivalis/aislamiento & purificación , Treponema denticola/aislamiento & purificación , Adulto , Distribución de Chi-Cuadrado , Periodontitis Crónica/diagnóstico , Periodontitis Crónica/microbiología , Recuento de Colonia Microbiana , Sondas de ADN , Encuestas de Salud Bucal , Placa Dental/microbiología , Pruebas de Enzimas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Hibridación de Ácido Nucleico/métodos , Enfermedades Periodontales/microbiología , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Factores de TiempoRESUMEN
The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54 percent (T0), 65.18 percent (T1), 65.22 percent (T2) and 50.26 percent (T3). The specificity values were 12.24 percent (T0), 57.38 percent (T1), 46.27 percent (T2) and 53.48 percent (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis.
Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Enfermedades Periodontales/diagnóstico , Porphyromonas gingivalis/aislamiento & purificación , Treponema denticola/aislamiento & purificación , Distribución de Chi-Cuadrado , Recuento de Colonia Microbiana , Periodontitis Crónica/diagnóstico , Periodontitis Crónica/microbiología , Encuestas de Salud Bucal , Sondas de ADN , Placa Dental/microbiología , Pruebas de Enzimas , Hibridación de Ácido Nucleico/métodos , Enfermedades Periodontales/microbiología , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Factores de TiempoRESUMEN
Vários estudos longitudinais têm reportado uma alta taxa de sucesso da utilização de implantes osseointegrados no tratamento reabilitador. Entretanto, outras investigações têm mostrado a perda de implantes osseointegrados por causa de infecções periimplantares ou trauma oclusal. A periimplantite é caracterizada pela presença de reações inflamatórias que afetam o tecido periimplantar sob função. Indivíduos portadores de periimplantes são colonizados por patógenos periodontais como Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum e Treponema denticola, enquanto sítios periimplantares sadios são colonizados por cocos Gram-positivos.
Longitudinal studies have reported a high success rate for rehabilitating treatment through the use of osseointegrated implants. However, other studies have reported osseointegrated implant failure due to peri-implant infections or occlusal overload. Peri-implantitis is characterized by the presence of inflammatory reactions that affect the peri-implant tissue under loading. Subjects with peri-implantitis harbor periodontal pathogens such as Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum and Treponema denticola while healthy peri-implant sites harbor cocos Gram-positives.