RESUMEN

Three sequential batch reactors (SBR) were operated to evaluate salt addition's impact on granulation, performance, and biopolymer production in aerobic granular sludge (AGS) systems. System R1 was fed without adding salt (control); system R2 operated with saline pulses, i.e., one cycle with salt (2.5 g NaCl/L) addition followed by another without salt; and R3 received continuous supplementation of 2.5 g NaCl/L. The results indicated that the reactors supplemented with salt presented higher concentrations of mixed liquor volatile suspended solids (MLVSS) and better settleability than R1, showing that osmotic pressure contributed to biomass growth, accelerated granulation, and improved physical characteristics. The faster granulation occurred in R2, thus proving the beneficial effects of intermittent salt addition through alternating pulses. Salt addition did not impair the simultaneous removal of carbon, nitrogen, and phosphorus. In fact, R2 showed better carbon removals. In conclusion, continuous or intermittent (pulsed) supplementation of 2.5 g NaCl/L did not lead to increased production of extracellular polymeric substances (EPS) and alginate-like exopolymers (ALE). This outcome could be attributed to the low saline concentration employed, a higher food-to-microorganism (F/M) ratio observed in R1, and possibly greater endogenous consumption of biopolymers in the famine period in R2 and R3 due to the greater solids retention time (SRT). Therefore, this study brings important results that contribute to a better understanding of the effect of salt in continuous dosing or in pulses as a selection pressure strategy to accelerate granulation, as well as the behavior of the AGS systems for saline effluents.

Asunto(s)

Reactores Biológicos , Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Aguas del Alcantarillado/química , Eliminación de Residuos Líquidos/métodos , Fósforo , Aerobiosis , Biomasa , Nitrógeno , Biopolímeros , Carbono/metabolismo , Estrés Salino , Cloruro de Sodio

RESUMEN

Aerobic granular sludge (AGS) systems have great potential for biopolymers recovery, especially when subjected to adverse conditions. This work aimed to study the production of alginate-like exopolymers (ALE) and tryptophan (TRY) under osmotic pressure in conventional and staggered feeding regimes. The results revealed that systems operated with conventional feed accelerated the granulation, although less resistant to saline pressures. The staggered feeding systems favored better denitrification conditions and long-term stability. Salt addition gradient increase influenced biopolymers' production. However, staggered feeding, despite decreasing the famine period, did not influence the production of resources and extracellular polymeric substances (EPS). Sludge retention time (SRT), which was not controlled, proved to be an important operational parameter with negative influences on biopolymers' production in values greater than 20 days. Thus, the principal component analysis confirmed that the production of ALE at low SRT is related to better-formed granules with good sedimentation characteristics and good AGS performances.

Asunto(s)

Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Eliminación de Residuos Líquidos/métodos , Presión Osmótica , Reactores Biológicos , Aerobiosis , Alginatos , Biopolímeros

RESUMEN

The influence of salt addition to stimulating biopolymers production in aerobic granular sludge (AGS) systems was evaluated. The control systems (R1: acetate and R2: propionate) initially obtained less accumulation of mixed liquor volatile suspended solids (MLVSS), indicating that the osmotic pressure in the salt-supplemented systems (R3: acetate and R4: propionate) contributed to biomass growth. However, the salt-supplemented systems collapsed between days 110 and 130 of operation. R3 and R4 showed better performance regarding nutrients removal due to the greater abundance of nitrifying and denitrifying bacteria and phosphate-accumulating organisms. Salt also contributed to the higher production of biopolymers such as alginate-like exopolymers (ALE) per gram of volatile suspended solids (VSS) (R1: 397 mgALE∙gVSS-1, R2: 140 mgALE∙gVSS-1, R3: 483 mgALE∙gVSS-1, R4: 311 mgALE∙gVSS-1). Amino acids like tyrosine and tryptophan were better identified in extracellular polymeric substances extract from salt-operated reactors. This study brings important results in the context of resource recovery by treating saline effluents.

Asunto(s)

Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Aguas del Alcantarillado/química , Eliminación de Residuos Líquidos/métodos , Reactores Biológicos/microbiología , Propionatos , Biopolímeros , Cloruro de Sodio , Aerobiosis

RESUMEN

This study evaluated the influence of carbon sources on alginate-like exopolymers (ALE) and tryptophan (Trp) biosynthesis in the aerobic granular sludge (AGS). With acetate, the highest biopolymers levels, per gram of volatile suspended solids (VSS) (418.7 mgALE∙g-1 and 4.1 mgTrp∙gVSS-1), were found likely due to biomass loss throughout the operation, which resulted in lower sludge age (4-7 days) and shorter famine period. During granulation, encouraging results on ALE production were obtained with propionate (>250 mgALE∙gVSS-1), significantly higher than those found with glycerol, glucose, and sucrose. Regarding tryptophan production, propionate and glycerol proved to be good substrates, although the content was still lower than acetate (1.6 mgTrp∙gVSS-1). Granules fed with glucose showed the worst results compared to the other substrates (38.5 mgALE∙VSS-1 and 0.6 mgTrp∙gVSS-1) due to the filamentous microorganisms' abundance found. Therefore, this study provides insights to value the production of compounds of industrial interest in AGS systems.

Asunto(s)

Aguas del Alcantarillado , Aguas Residuales , Aerobiosis , Alginatos , Reactores Biológicos , Carbono , Glucosa , Glicerol , Propionatos , Aguas del Alcantarillado/química , Triptófano , Eliminación de Residuos Líquidos/métodos