RESUMEN
Comparative study of hepatitis C virus (HCV) markers (core protein, RNA, and virus-specific antibodies) was carried out in plasma samples from 80 donors. A method based on sandwich ELISA with monoclonal antibodies to recombinant protein was developed for measuring core protein. Nucleocapsid protein was detected after various treatments of precipitates obtained after concentration of virus-containing material from plasma samples. These treatments allowed differentiation of core protein in virions, free nucleocapsids, and immune complexes circulating in peripheral blood. The minimal detectable concentration was 5 pg/ml, maximal 850 pg/ml. The detection of core protein virtually coincided with the detection of HCV RNA: 94.4% RNA-positive samples contained the virus protein. Other parameters (activities of antibodies to HCV in ELISA and level of SGPT) did not allow differentiation of plasma samples by the presence of actively replicating virus. Assay of nucleocapsid protein in the plasma of subjects infected with HCV in various populations of virus particles is important from practical (for blood service) and theoretical viewpoints (for studies of virus pathogenesis mechanisms).
Asunto(s)
Anticuerpos Antivirales/sangre , Biomarcadores/sangre , Donantes de Sangre , Hepatitis C/genética , Proteínas de la Nucleocápside/sangre , ARN Viral/sangre , Ensayo de Inmunoadsorción Enzimática , Hepatitis C/inmunología , HumanosRESUMEN
Five types of monoclonal antibodies (Mab) to Inkoo virus were obtained. The direction of Mabs to virus proteins was determined and reactions of Mabs with other California group viruses studied by the fluorescent antibody method.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Bunyaviridae/clasificación , Animales , Anticuerpos Monoclonales/biosíntesis , Western Blotting , Bunyaviridae/inmunología , Técnica del Anticuerpo Fluorescente , Hibridomas/inmunología , Ratones , Ratones Endogámicos BALB C , Especificidad de la Especie , Células Tumorales Cultivadas , Proteínas Virales/inmunologíaRESUMEN
Comparative analysis of the characteristics of tick-borne encephalitis (TBE) strain 205 used for the production of vaccine against TBE and of its variants obtained by passages in mouse brain showed the stability of such properties as infective activity, neurovirulence, sensitivity to physical (heating to 50 C) and chemical (sodium deoxycholate treatment) factors. At the same time increased neurovirulence of variants 205/M10 and 205/M20, which undergone through 10 and 20 passages in white mouse brain, for low-sensitive Syrian hamsters was revealed. Use of a panel of 5 monoclonal antibodies to protein E and of 4 monoclonal antibodies to protein E and of 4 monoclonal antibodies to protein NS3 helped differentiate between not only strains 205 and Sofyin, but between variants of strain 205 as well.