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The production of keratinases was evaluated in submerged fermentation with Aspergillus niger and by pigs' swine hair in a batch bioreactor. Experimental planning was performed to assess the interaction between different variables. The enzyme extract produced was characterized at various pH and temperatures and subjected to enzyme concentration using a biphasic aqueous system and salt/solvent precipitation techniques. In addition, the substrate's potential in reducing hexavalent chromium from synthetic potassium dichromate effluent with an initial concentration of 20 mg L-1 of chromium was evaluated. The resulting enzyme extract showed 89 ± 2 U mL-1 of keratinase. The enzyme concentration resulted in a purification factor of 1.3, while sodium chloride/acetone and ammonium sulfate/acetone resulted in a purification factor of 1.9 and 1.4, respectively. Still using the residual substrate of swine hair from the fermentation, a 94% reduction of hexavalent chromium concentration occurred after 9 h of reaction. Thus, the study proved relevant for producing keratinases, with further environmental applicability and the possibility of concentrating the extract via low-cost processes.

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Brazil is one of the world's largest consumers of pesticides. This intense use impacts the environment and exposes a wide range of individuals to pesticides, including rural workers who are occupationally exposed and rural residents who are environmentally exposed. We aimed to evaluate the effects of occupational exposure to pesticides on the health of rural workers and rural residents. We conducted an epidemiological study with 104 farmers and 23 rural residents of Casimiro de Abreu (Rio de Janeiro, Brazil). A comparison group (urban residents) comprised 103 residents of the urban area of the same city. We determined the activity of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) using a modified version of Ellman's method to evaluate exposure. In addition, we performed genotoxic and mutagenic analyses with the comet assay and the cytokinesis-block micronucleus (CBMN) assay. There was a reduction in cholinesterase activity, mainly BChE, in rural workers and rural residents compared with urban residents (p = 0.002). There was an increase in genotoxic effects in rural workers compared with urban residents (comet assay, p < 0.001; CBMN assay, p < 0.001). In addition, there was a greater chance of genotoxic changes in rural workers exposed to pesticides based on the comet assay (odds ratio [OR] 7.6, 95 % confidence interval [CI] 6.6-15.9) and the CBMN assay (OR 22.7, 95 % CI 10.3-49.9). We found that individuals occupationally exposed to pesticides are more likely to have genotoxic effects. These findings are useful for the development of programs to monitor populations exposed to genotoxic substances and allow the development of strategies for the prevention, control, and surveillance of effects that result from occupational and environmental exposures to pesticides.

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During scaling of fermentations, choosing a bioreactor is fundamental to ensure the product's quality. This study aims to produce bioherbicides using Trichoderma koningiopsis fermentation, evaluating process parameters in an Airlift bioreactor. As a response, we quantified the production of enzymes involved in the bioherbicide activity (amylase, cellulase, laccase, lipase, and peroxidase). In addition, it evaluated the agronomic efficiency of the fermented extract optimized through tests that promoted soybean growth and nodulation, soybean seed germination, and in vitro phytopathogen control. As a result of optimizing the scaling bioprocess, it was possible to obtain an adequate fermentation condition, which, when applied to soybean seeds, had beneficial effects on their growth. It allowed the production of an enzyme cocktail. These results add a crucial biotechnological potential factor for the success of the optimized formulation in the Airlift bioreactor, in addition to presenting relevant results for the scientific community.

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The general population and workers are exposed to organophosphate insecticides, one of the leading chemical classes of pesticides used in rural and urban areas. This paper aims to conduct an integrative review of the most used analytical methods for identifying and quantifying dialkylphosphate-which are metabolites of organophosphate insecticides-in the urine of exposed workers, discussing their advantages, limitations and applicability. Searches utilized the PubMed, the Scientific Electronic Library Online and the Brazilian Digital Library of Theses and Dissertations databases between 2000 and 2021. Twenty-five studies were selected. The extraction methods most used were liquid-liquid extraction (LLE) (36%) and solid-phase extraction (SPE) (36%), with the SPE being more economical in terms of time and amount of solvents needed, and presenting the best percentage of recovery of analytes, when compared with LLE. Nineteen studies (76%) used the gas chromatography method of separation, and among these, 12 records (63%) indicated mass spectrometry used as a detection technology (analyzer). Studies demonstrate that dialkylphosphates are sensitive and representative exposure biomarkers for environmental and occupational organophosphate exposure.

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The general population and workers are exposed to organophosphate insecticides, one of the leading chemical classes of pesticides used in rural and urban areas, in the control of arboviruses and agriculture. These pesticides cause environmental/occupational exposure and associated risks to human and environmental health. The objective of this study was to carry out an integrative review of epidemiological studies that identified and quantified dialkylphosphate metabolites in the urine of exposed populations, focusing on the vector control workers, discussing the application and the results found. Searches utilized the Pubmed, Scielo, and the Brazilian Digital Library of Theses and Dissertations (BDTD) databases between 2000 and 2021. From the 194 selected studies, 75 (39%) were with children/adolescents, 48 (24%) with rural workers, 36 (19%) with the general population, 27 (14%) with pregnant women, and 9 (4%) with vector control workers. The total dialkylphosphate concentrations found in the occupationally exposed population were higher than in the general population. Studies demonstrate that dialkylphosphates are sensitive and representative exposure biomarkers for environmental and occupational organophosphate exposure. The work revealed a lack of studies with vector control workers and a lack of studies in developing countries.

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The literature is full of studies reporting environmental and health issues related to using traditional pesticides in food production and storage. Fortunately, alternatives have arisen in the last few decades, showing that organic agriculture is possible and economically feasible. And in this scenario, fungi may be helpful. In the natural environment, when associated with plants, these microorganisms offer plant-growth-promoting molecules, facilitate plant nutrient uptake, and antagonize phytopathogens. It is true that fungi can also be phytopathogenic, but even they can benefit agriculture in some way-since pathogenicity is species-specific, these fungi are shown to be useful against weeds (as bioherbicides). Finally, plant-associated yeasts and molds are natural biofactories, and the metabolites they produce while dwelling in leaves, flowers, roots, or the rhizosphere have the potential to be employed in different industrial activities. By addressing all these subjects, this manuscript comprehensively reviews the biotechnological uses of plant-associated fungi and, in addition, aims to sensitize academics, researchers, and investors to new alternatives for healthier and more environmentally friendly production processes.

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A Cannabis sativa é uma planta que apresenta vários benefícios terapêuticos para animais, como tratamento da dor neuropática, inflamatória e osteoartrose. A dor é bastante recorrente na rotina clínica, sendo importante seu manejo para que seja ofertada uma melhor qualidade e conforto de vida para o paciente. O estudo objetivou identificar, a partir de evidências científicas, as características da utilização medicinal do uso de Cannabis Sativa no tratamento da dor crônica no cão, utilizando um dos seus princípios ativos, canabidiol (CBD). Foi feito uma revisão bibliográfica onde foi realizada a busca de estudos experimentais e relatos de caso em bases de dados eletrônicos, sendo incluídas fontes contendo a utilização do CBD em animais, que abordaram controle da dor, assim como escore avaliativo da dor antes, durante e após o tratamento proposto. Após eleger e analisar 54 estudos percebe-se que na medicina veterinária o uso do canabidio é insuficiente, uma vez que o foco da maior parte dos estudos clínicos é voltado para medicina humana. Ainda assim, a utilização de CBD mostrou-se eficaz, confirmando uma nova alternativa para o controle da dor em animais.(AU)

Cannabis sativa is a plant that has several therapeutic benefits for animals, such as the treatment of neuropathic and inflammatory pain and osteoarthritis. Pain is quite recurrent in the clinical routine, and its management is important to offer a better quality and comfort of life for the patient. The study aimed to identify, based on scientific evidence, the characteristics of the medicinal use of Cannabis Sativa in the treatment of chronic pain in dogs, using one of its active principles, cannabidiol (CBD). A bibliographical review was carried out in which experimental studies and case reports were searched in electronic databases, including sources containing the use of CBD in animals, which addressed pain control, as well as pain assessment score before, during and after the proposed treatment. After choosing and analyzing 54 studies, it is clear that in veterinary medicine the use of CBD is few, and the focus of clinical studies is on human medicine. The use of CBD proved to be effective, thus confirming a new alternative for pain control in animals.(AU)

El cannabis sativa es una planta que tiene varios beneficios terapéuticos para los animales, como el tratamiento del dolor neuropático e inflamatorio y la osteoartritis. El dolor es bastante recurrente en la rutina clínica, y su manejo es importante para ofrecer una mejor calidad y comodidad de vida al paciente. El estudio tuvo como objetivo identificar, con base en la evidencia científica, las características del uso medicinal de Cannabis Sativa en el tratamiento del dolor crónico en perros, utilizando uno de sus principios activos, el cannabidiol (CBD). Se realizó una revisión bibliográfica en la que se buscaron estudios experimentales y reportes de casos en bases de datos electrónicas, incluyendo fuentes que contengan el uso de CBD en animales, que abordaran el control del dolor, así como la puntuación de evaluación del dolor antes, durante y después del tratamiento propuesto. Después de elegir y analizar 54 estudios, queda claro que en medicina veterinaria el uso de cannabidio es insuficiente, ya que el foco de la mayoría de los estudios clínicos está en la medicina humana. Aun así, el uso de CBD demostró ser efectivo, confirmando una nueva alternativa para el control del dolor en animales.(AU)

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O herpesvírus da espécie Psittacid alphaherpesvirus 1 (PsHV-1), é o responsável pela doença aviária altamente infecciosa e aguda, descrita como "Doença de Pacheco" (DP). Diversas espécies de psitacídeos (papagaios da Amazônia, seguido por papagaios cinzentos africanos, papagaios comuns, araras, cacatuas e algumas espécies de periquitos), são suscetíveis à doença, principalmente àquelas oriundas de criadouros que deram entrada em centros de reabilitações em quaisquer regiões geográficas. Objetivou- se com o presente estudo avaliar e discutir as ocorrências da "Doença de Pacheco" em psitaciformes descritas no Brasil e em outros países, pretendendo-se discernir sobre as causas da infecção, discorrendo sobre as causas de contágio e disseminação, descrevendo brevemente a sintomatologia, possíveis lesões, diagnósticos, profilaxia e tratamento, a fim de evitar o contágio, minimizando a morbidade e mortalidade das aves. Trata-se de uma revisão bibliográfica, a qual foi realizada por meio de consultas à periódicos e livros mais recentes. Trata-se de uma revisão bibliográfica, em que foram utilizadas as bases de dados da SciELO, portal Capes e Google Acadêmico para realizar a revisão em artigos, monografias, teses e dissertações de vários autores e livros. Pouco se conhece, e nenhum registro ainda foi reportado para a doença no Brasil, apesar de sua ocorrência ser amplamente divulgada em diversos países. Os principais sinais clínicos são anorexia, sonolência, letargia, penas eriçadas, diarreia amarelada, regurgitação, inatividade e, às vezes, sinais nervosos, chegando, por fim, à morte súbita e rápida. Na necropsia, podem ser achados hepatomegalia, esplenomegalia e necrose. A profilaxia se concentra no controle da superpopulação e protocolo adequado de quarentena das aves. O tratamento indicado para o herpesvírus é o uso de nucleosídeo sintético, com atividade inibitória, o aciclovir, que tem apresentado bons resultados na redução das taxas de mortalidade.(AU)

The herpesvirus of the species Psittacid alphaherpesvirus 1 (PsHV-1), is responsible for the highly infectious and acute avian disease, described as "Pacheco's Disease" (PD). Several species of parrots (Amazon parrots, followed by African gray parrots, common parrots, macaws, cockatoos and some species of parakeets) are susceptible to the disease, especially those originating from breeding sites that have entered rehabilitation centers in any region. geographic. The aim of the present study was to evaluate and discuss the occurrences of "Pacheco's Disease" in parrots described in Brazil and in other countries, intending to discern the causes of the infection, discussing the causes of contagion and dissemination, briefly describing the symptomatology, possible lesions, diagnosis, prophylaxis and treatment, in order to avoid contagion, minimizing the morbidity and mortality of the birds. This is a bibliographic review, which was carried out through consultations with the most recent journals and books. This is a bibliographic review, in which the SciELO databases, Capes portal and Google Scholar were used to review articles, monographs, theses and dissertations by various authors and books. Little is known, and no record has yet been reported for the disease in Brazil, despite its occurrence being widely publicized in several countries. The main clinical signs are anorexia, drowsiness, lethargy, ruffled feathers, yellowish diarrhea, regurgitation, inactivity and, sometimes, nervous signs, finally leading to sudden and rapid death. At necropsy, hepatomegaly, splenomegaly and necrosis may be found. Prophylaxis focuses on overpopulation control and proper bird quarantine protocol. The treatment indicated for herpesvirus is the use of a synthetic nucleoside, with inhibitory activity, acyclovir, which has shown good results in reducing mortality rates.(AU)

El herpesvirus de la especie Psittacid alphaherpesvirus 1 (PsHV-1), es responsable de la enfermedad aviar aguda y altamente infecciosa, descrita como "Enfermedad de Pacheco" (EP). Varias especies de loros (loros amazónicos, seguidos de loros grises africanos, loros comunes, guacamayos, cacatúas y algunas especies de periquitos) son susceptibles a la enfermedad, en especial los que se originan en criaderos que han ingresado a centros de rehabilitación en cualquier región geográfica. El objetivo del presente estudio fue evaluar y discutir las ocurrencias de la "Enfermedad de Pacheco" en loros descritas en Brasil y en otros países, con la intención de discernir las causas de la infección, discutiendo las causas de contagio y diseminación, describiendo brevemente la sintomatología, posibles lesiones, diagnóstico, profilaxis y tratamiento, con el fin de evitar el contagio, minimizando la morbimortalidad de las aves. Se trata de una revisión bibliográfica, que se realizó mediante consultas a las revistas y libros más recientes. Se trata de una revisión bibliográfica, en la que se utilizaron las bases de datos SciELO, el portal Capes y Google Scholar para revisar artículos, monografías, tesis y disertaciones de diversos autores y libros. Se sabe poco y aún no se ha informado de ningún registro de la enfermedad en Brasil, a pesar de que su aparición es ampliamente publicitada en varios países. Los principales signos clínicos son anorexia, somnolencia, letargo, plumas erizadas, diarrea amarillenta, regurgitación, inactividad y, en ocasiones, signos nerviosos, que finalmente conducen a la muerte súbita y rápida. En la necropsia, se pueden encontrar hepatomegalia, esplenomegalia y necrosis. La profilaxis se centra en el control de la sobrepoblación y el protocolo adecuado de cuarentena de aves. El tratamiento indicado para el herpesvirus es el uso de un nucleósido sintético, con actividad inhibidora, el aciclovir, que ha mostrado buenos resultados en la reducción de la mortalidad.(AU)

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The importance of insects for angiosperm pollination is widely recognized. In fact, approximately 90% of all plant species benefit from animal-mediated pollination. However, only recently, a third part player in this story has been properly acknowledged. Microorganisms inhabiting floral nectar, among which yeasts have a prominent role, can ferment glucose, fructose, sucrose, and/or other carbon sources in this habitat. As a result of their metabolism, nectar yeasts produce diverse volatile organic compounds (VOCs) and other valuable metabolites. Notably, some VOCs of yeast origin can influence insects' foraging behavior, e.g., by attracting them to flowers (although repelling effects have also been reported). Moreover, when insects feed on nectar, they also ingest yeast cells, which provide them with nutrients and protect them from pathogenic microorganisms. In return, insects serve yeasts as transportation and a safer habitat during winter when floral nectar is absent. From the plant's point of view, the result is flowers being pollinated. From humanity's perspective, this ecological relationship may also be highly profitable. Therefore, prospecting nectar-inhabiting yeasts for VOC production is of major biotechnological interest. Substances such as acetaldehyde, ethyl acetate, ethyl butyrate, and isobutanol have been reported in yeast volatomes, and they account for a global market of approximately USD 15 billion. In this scenario, the present review addresses the ecological, environmental, and biotechnological outlooks of this three-party mutualism, aiming to encourage researchers worldwide to dig into this field.

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Aiming to broaden the base of knowledge about wild yeasts, four new indigenous strains were isolated from corn residues, and phylogenetic-tree assemblings on ITS and LSU regions indicated they belong to Meyerozyma caribbica. Yeasts were cultivated under full- and micro-aerobiosis, starting with low or high cell-density inoculum, in synthetic medium or corn hydrolysate containing glucose and/or xylose. Cells were able to assimilate both monosaccharides, albeit by different metabolic routes (fermentative or respiratory). They grew faster in glucose, with lag phases ~ 10 h shorter than in xylose. The hexose exhaustion occurred between 24 and 34 h, while xylose was entirely consumed in the last few hours of cultivation (44-48 h). In batch fermentation in synthetic medium with high cell density, under full-aerobiosis, 18-20 g glucose l-1 were exhausted in 4-6 h, with a production of 6.5-7.0 g ethanol l-1. In the xylose medium, cells needed > 12 h to consume the carbohydrate, and instead of ethanol, cells released 4.4-6.4 g l-1 xylitol. Under micro-aerobiosis, yeasts were unable to assimilate xylose, and glucose was more slowly consumed, although the ethanol yield was the theoretical maximum. When inoculated into the hydrolysate, cells needed 4-6 h to deplete glucose, and xylose had a maximum consumption of 57%. Considering that the hydrolysate contained ~ 3 g l-1 acetic acid, it probably has impaired sugar metabolism. Thus, this study increases the fund of knowledge regarding indigenous yeasts and reveals the biotechnological potential of these strains.

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Gasoline is a complex mixture of substances, including aromatic hydrocarbons such as benzene, toluene, ethylbenzene, and xylenes (BTEX). These compounds are emitted into the air, with the special relevance of benzene since it is provenly carcinogenic. The study aimed to assess BTEX concentrations in filling stations in the city of Rio de Janeiro, Brazil, and to calculate the cancer risk associated with such exposures. Two types of sampling were performed (stationary and mobile), adapted from methodology n. 1,501 (U.S. National Institute for Occupational Safety and Health) for aromatic hydrocarbons, in six filling stations in the West Zone of Rio de Janeiro. Stationary sampling was done near the fuel pumps, while mobile sampling was done in the breathing zone of the workers (station attendants) as they moved around the station. The samples were analyzed with gas chromatography flame ionization detector. The sampling results were used to calculate the health risk, using the indicators Hazard quotient (HQ) and Cancer risk (CR) to assess the possible non-carcinogenic and carcinogenic effects, respectively, in filling station workers. Environmental concentrations for the most of the BTEX compounds were below the recommended limits, except for benzene, a carcinogenic compound, which displayed concentrations far above the limits, leading to high cancer risk values. The results showed that there are health risks for filling station attendants, especially the risk of developing cancer from excessive exposure to benzene.

A gasolina é uma mistura complexa de substâncias, dentre elas estão hidrocarbonetos como o benzeno, tolueno, etilbenzeno e xilenos (BTEX), sendo esses compostos emitidos para a atmosfera, com destaque ao benzeno, por ser comprovadamente carcinogênico. Objetivamos avaliar as concentrações de BTEX no ar de postos de revenda de combustíveis no Município do Rio de Janeiro, Brasil, e calcular o risco de câncer associado a estas exposições. Foram realizados dois tipos de amostragem (fixa e móvel) adaptadas da metodologia nº 1.501 do Instituto Nacional de Segurança e Saúde Ocupacional (Estados Unidos), em seis postos de revenda de combustíveis na Zona Oeste da cidade. A amostragem fixa foi feita próxima às bombas de combustível, enquanto a móvel foi realizada através da coleta do ar na zona respiratória dos trabalhadores (frentistas), conforme estes se deslocavam pelo posto. As amostras foram analisadas por cromatografia gasosa com detecção por ionização em chama. Com os resultados das amostragens foram feitos os cálculos de risco à saúde, usando os indicadores Quociente de perigo (HQ) e Risco de câncer (CR), para avaliar os possíveis efeitos não-carcinogênicos e carcinogênicos, respectivamente, nos trabalhadores dos postos de revenda de combustíveis. As concentrações ambientais para a maioria dos compostos BTEX foram abaixo dos limites preconizados, menos para o benzeno, um composto carcinogênico, que apresentou concentrações muito acima dos limites, levando a altos valores de risco de câncer. Os resultados demonstraram que há riscos à saúde dos trabalhadores de postos de revenda de combustíveis, principalmente o risco de desenvolver câncer, devido à exposição excessiva ao benzeno.

La gasolina es una mezcla compleja de sustancias, entre ellas existen hidrocarburos como el benceno, tolueno, etilbenceno y xilenos (BTEX), emitiéndose estos compuestos a la atmósfera, donde se destaca el benceno, al tratarse de un carcinogénico comprobado. Los objetivos fueron evaluar las concentraciones de BTEX en el aire de puestos de reventa de combustibles, en el municipio de Río de Janeiro, Brasil, así como calcular el riesgo de cáncer asociado a estas exposiciones. Se realizaron dos tipos de muestra (fija y movible), adaptadas de la metodología nº 1.501 del Instituto Nacional de Seguridad y Salud Laboral (EE.UU.), en seis puestos de reventa de combustibles de la zona oeste de la ciudad. La muestra fija se realizó cerca de las bombas de combustible, mientras que la móvil se hizo mediante tomas de aire en la zona respiratoria de los trabajadores (de la gasolinera), a medida que estos se desplazaban por el lugar de trabajo. Las muestras se analizaron por cromatografía gaseosa con detección por ionización en llama. Junto a los resultados de las muestras, se realizaron cálculos de riesgo para la salud, usando los indicadores Cociente de peligro (HQ) y Riesgo de cáncer (CR), con el fin de evaluar los posibles efectos no-carcinogénicos y carcinogénicos, respectivamente, en los trabajadores de los puestos de reventa de combustibles. Las concentraciones ambientales para la mayoría de los compuestos BTEX estuvieron por debajo de los limites preconizados, menos en el caso del benceno, un compuesto carcinogénico, que presentó concentraciones muy por encima de los límites, llevando a altos valores de riesgo de cáncer. Los resultados demostraron que existen riesgos para la salud de los trabajadores de puestos de reventa de combustibles, principalmente, riesgo de desarrollar cáncer, debido a la exposición excesiva al benceno.

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Mangroves are ecosystems with unique characteristics due to the high salinity and amount of organic matter that house a rich biodiversity. Fungi have aroused much interest as they are an important natural source for the discovery of new bioactive compounds, with potential biotechnological and pharmacological interest. This review aims to highlight endophytic fungi isolated from mangrove plant species and the isolated bioactive compounds and their bioactivity against protozoa, bacteria and pathogenic viruses. Knowledge about this type of ecosystem is of great relevance for its preservation and as a source of new molecules for the control of pathogens that may be of importance for human, animal and environmental health.

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The objective of this study was to evaluate the ethanol production by Wickerhamomyces sp. using soybean straw and hull hydrolysates obtained by subcritical water hydrolysis and, afterward, the biogas production using the fermented hydrolysates. Ethanol was produced using the straw and hull hydrolysates diluted and supplement with glucose, reaching 5.57 ± 0.01 g/L and 6.11 ± 0.11 g/L, respectively. The fermentation in a bioreactor with changing the pH to 7.0 allowed achieving maximum ethanol production of 4.03 and 3.60 g/L for straw and hull hydrolysates at 24 h, respectively. The biogas productions obtained for the fermented hydrolysates of straw with and without changing the pH were 739 ± 37 and 652 ± 34 NmL/gVSad, respectively. The fermented hydrolysate of hull without changing the pH presented 620 ± 26 NmL/gVSad. The soybean residues produced biofuels, indicating these residues show potential as raw material for renewable energy production.

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Seawater (SW) and wastewater from shrimp production (WSP) were used as a solvent for the fermentation of papaya residues (Carica papaya) by Wickerhamomyces sp. UFFS-CE-3.1.2 and Saccharomyces cerevisiae CAT-1. For comparative purposes and evaluation of the effect of salinity, ultrapure water (UW) was used as control. Fermentative parameters were evaluated in Plackett-Burman planning to assess ethanol production's significant variables. Urea supplementation was the only variable not significant for the proposed process, suggesting that papaya residues contain all the nutrients needed for fermentation. The experiments conducted with the different water sources resulted in similar concentrations of ethanol. Maximum ethanol concentration was obtained after nine h of fermentation usingWickerhamomycessp. UFFS-CE-3.1.2 (27.31 ± 1.40 g L-1) and 12 h using S. cerevisiaeCAT-1 (24.53 ± 0.68 g L-1). This study demonstrated that SW and WSP could replace freshwater without affecting ethanol production. Papaya residues from the fruit and vegetable sectors can be considered a promising substrate source for ethanol production.

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Grazing intensity on palisade grass as a function of grazing height in a crop-livestock integration (CLI) system alters the structural soil quality and water infiltration. This study aimed to verify the magnitude of the stocking rate and shoot and root dry matter of palisade grass at the end of the fifth cattle grazing period of an experiment in the CLI system on the visual evaluation of soil structure (VESS), soil-water infiltration rate, and basic infiltration rate (BIR) as a function of grazing heights on Urochloa brizantha cv. MG 5 Vitória in the northwestern Paraná State, Brazil. The experimental design consisted of randomized blocks with three replications and 1-ha experimental plots on an Oxisol. Four treatments with grazing heights of 10, 20, 30, and 40 cm were used in the CLI system, controlled with variable stocking rates of Purunã cattle in the autumn/winter season on Urochloa brizantha cv. MG 5 Vitória in succession to soybean in the summer. The maximum soil water infiltration was stabilized at 30 cm of grazing height of the palisade grass under continuous grazing in the CLI system. The increase in the stocking rate decreased the shoot dry matter of palisade grass, soil water infiltration, and structural soil quality.

A intensidade de pastejo da braquiária em função da altura de pastejo em sistema de integração lavourapecuária (ILP) altera a qualidade estrutural do solo e a infiltração da água. Com este trabalho teve-se por objetivo verificar ao final do quinto período de pastejo bovino de um experimento em sistema de ILP, qual a magnitude da carga animal, matéria seca da parte aérea e de raízes de braquiária, sobre a avaliação visual da estrutura do solo (VESS), a taxa de infiltração de água no solo e a velocidade básica de infiltração (VIB) em função da alturas de pastejos em braquiária Urochloa brizantha cv. MG 5 Vitória, na Região Noroeste do Estado do Paraná, Brasil. O delineamento experimental é de blocos casualizados, com três repetições, em parcelas experimentais de 1 ha, em um Latossolo Vermelho distrófico. Foram utilizados quatro tratamentos com alturas de pastejo de 10, 20, 30 e 40 cm em sistema de ILP controlados com carga animal variável de bovinos da raça Purunã no período de outono/inverno, em braquiária Urochloa brizantha cv. MG 5 Vitória em sucessão a soja no verão. A máxima infiltração da água no solo se estabiliza em 30 cm de altura de pastejo da braquiária em pastejo contínuo em sistema ILP. O aumento da carga animal diminuiu a matéria seca da parte aérea de braquiária, infiltração de água e a qualidade estrutural do solo.

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Grazing intensity on palisade grass as a function of grazing height in a crop-livestock integration (CLI) system alters the structural soil quality and water infiltration. This study aimed to verify the magnitude of the stocking rate and shoot and root dry matter of palisade grass at the end of the fifth cattle grazing period of an experiment in the CLI system on the visual evaluation of soil structure (VESS), soil-water infiltration rate, and basic infiltration rate (BIR) as a function of grazing heights on Urochloa brizantha cv. MG 5 Vitória in the northwestern Paraná State, Brazil. The experimental design consisted of randomized blocks with three replications and 1-ha experimental plots on an Oxisol. Four treatments with grazing heights of 10, 20, 30, and 40 cm were used in the CLI system, controlled with variable stocking rates of Purunã cattle in the autumn/winter season on Urochloa brizantha cv. MG 5 Vitória in succession to soybean in the summer. The maximum soil water infiltration was stabilized at 30 cm of grazing height of the palisade grass under continuous grazing in the CLI system. The increase in the stocking rate decreased the shoot dry matter of palisade grass, soil water infiltration, and structural soil quality.(AU)

A intensidade de pastejo da braquiária em função da altura de pastejo em sistema de integração lavourapecuária (ILP) altera a qualidade estrutural do solo e a infiltração da água. Com este trabalho teve-se por objetivo verificar ao final do quinto período de pastejo bovino de um experimento em sistema de ILP, qual a magnitude da carga animal, matéria seca da parte aérea e de raízes de braquiária, sobre a avaliação visual da estrutura do solo (VESS), a taxa de infiltração de água no solo e a velocidade básica de infiltração (VIB) em função da alturas de pastejos em braquiária Urochloa brizantha cv. MG 5 Vitória, na Região Noroeste do Estado do Paraná, Brasil. O delineamento experimental é de blocos casualizados, com três repetições, em parcelas experimentais de 1 ha, em um Latossolo Vermelho distrófico. Foram utilizados quatro tratamentos com alturas de pastejo de 10, 20, 30 e 40 cm em sistema de ILP controlados com carga animal variável de bovinos da raça Purunã no período de outono/inverno, em braquiária Urochloa brizantha cv. MG 5 Vitória em sucessão a soja no verão. A máxima infiltração da água no solo se estabiliza em 30 cm de altura de pastejo da braquiária em pastejo contínuo em sistema ILP. O aumento da carga animal diminuiu a matéria seca da parte aérea de braquiária, infiltração de água e a qualidade estrutural do solo.(AU)

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A gasolina é uma mistura complexa de substâncias, dentre elas estão hidrocarbonetos como o benzeno, tolueno, etilbenzeno e xilenos (BTEX), sendo esses compostos emitidos para a atmosfera, com destaque ao benzeno, por ser comprovadamente carcinogênico. Objetivamos avaliar as concentrações de BTEX no ar de postos de revenda de combustíveis no Município do Rio de Janeiro, Brasil, e calcular o risco de câncer associado a estas exposições. Foram realizados dois tipos de amostragem (fixa e móvel) adaptadas da metodologia nº 1.501 do Instituto Nacional de Segurança e Saúde Ocupacional (Estados Unidos), em seis postos de revenda de combustíveis na Zona Oeste da cidade. A amostragem fixa foi feita próxima às bombas de combustível, enquanto a móvel foi realizada através da coleta do ar na zona respiratória dos trabalhadores (frentistas), conforme estes se deslocavam pelo posto. As amostras foram analisadas por cromatografia gasosa com detecção por ionização em chama. Com os resultados das amostragens foram feitos os cálculos de risco à saúde, usando os indicadores Quociente de perigo (HQ) e Risco de câncer (CR), para avaliar os possíveis efeitos não-carcinogênicos e carcinogênicos, respectivamente, nos trabalhadores dos postos de revenda de combustíveis. As concentrações ambientais para a maioria dos compostos BTEX foram abaixo dos limites preconizados, menos para o benzeno, um composto carcinogênico, que apresentou concentrações muito acima dos limites, levando a altos valores de risco de câncer. Os resultados demonstraram que há riscos à saúde dos trabalhadores de postos de revenda de combustíveis, principalmente o risco de desenvolver câncer, devido à exposição excessiva ao benzeno.

Gasoline is a complex mixture of substances, including aromatic hydrocarbons such as benzene, toluene, ethylbenzene, and xylenes (BTEX). These compounds are emitted into the air, with the special relevance of benzene since it is provenly carcinogenic. The study aimed to assess BTEX concentrations in filling stations in the city of Rio de Janeiro, Brazil, and to calculate the cancer risk associated with such exposures. Two types of sampling were performed (stationary and mobile), adapted from methodology n. 1,501 (U.S. National Institute for Occupational Safety and Health) for aromatic hydrocarbons, in six filling stations in the West Zone of Rio de Janeiro. Stationary sampling was done near the fuel pumps, while mobile sampling was done in the breathing zone of the workers (station attendants) as they moved around the station. The samples were analyzed with gas chromatography flame ionization detector. The sampling results were used to calculate the health risk, using the indicators Hazard quotient (HQ) and Cancer risk (CR) to assess the possible non-carcinogenic and carcinogenic effects, respectively, in filling station workers. Environmental concentrations for the most of the BTEX compounds were below the recommended limits, except for benzene, a carcinogenic compound, which displayed concentrations far above the limits, leading to high cancer risk values. The results showed that there are health risks for filling station attendants, especially the risk of developing cancer from excessive exposure to benzene.

La gasolina es una mezcla compleja de sustancias, entre ellas existen hidrocarburos como el benceno, tolueno, etilbenceno y xilenos (BTEX), emitiéndose estos compuestos a la atmósfera, donde se destaca el benceno, al tratarse de un carcinogénico comprobado. Los objetivos fueron evaluar las concentraciones de BTEX en el aire de puestos de reventa de combustibles, en el municipio de Río de Janeiro, Brasil, así como calcular el riesgo de cáncer asociado a estas exposiciones. Se realizaron dos tipos de muestra (fija y movible), adaptadas de la metodología nº 1.501 del Instituto Nacional de Seguridad y Salud Laboral (EE.UU.), en seis puestos de reventa de combustibles de la zona oeste de la ciudad. La muestra fija se realizó cerca de las bombas de combustible, mientras que la móvil se hizo mediante tomas de aire en la zona respiratoria de los trabajadores (de la gasolinera), a medida que estos se desplazaban por el lugar de trabajo. Las muestras se analizaron por cromatografía gaseosa con detección por ionización en llama. Junto a los resultados de las muestras, se realizaron cálculos de riesgo para la salud, usando los indicadores Cociente de peligro (HQ) y Riesgo de cáncer (CR), con el fin de evaluar los posibles efectos no-carcinogénicos y carcinogénicos, respectivamente, en los trabajadores de los puestos de reventa de combustibles. Las concentraciones ambientales para la mayoría de los compuestos BTEX estuvieron por debajo de los limites preconizados, menos en el caso del benceno, un compuesto carcinogénico, que presentó concentraciones muy por encima de los límites, llevando a altos valores de riesgo de cáncer. Los resultados demostraron que existen riesgos para la salud de los trabajadores de puestos de reventa de combustibles, principalmente, riesgo de desarrollar cáncer, debido a la exposición excesiva al benceno.

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This study aimed to evaluate the occurrence of the green tobacco sickness (GTS) and its associated factors in tobacco familiar farmers residing in Dom Feliciano, Rio Grande do Sul State, Brazil. A cross-sectional study was conducted evaluating the sickness in 354 small tobacco farmers, between October 2011 and March 2012. The urinary concentration of cotinine, a biomarker of exposure to nicotine, was determined during the tobacco harvest period. Subjects presenting cotinine urinary levels ≥ 50ng/mL, that had contact with tobacco leaves up to 48 hours before the sample collection and reported at least one disease symptom were deemed as cases. A non-conditional logistic analysis was performed to evaluate the association between GTS and the population characteristics. A total of 122 (34.5%) cases were identified, with 39% of them being smokers and 61% being males. The median cotinine urinary concentrations were 75.6ng/mL (74.1ng/mg of creatinine) for non-cases and 755.8ng/mL (632.1ng/mg of creatinine) for the cases (p-value ≤ 0.01). The multivariate analysis showed a positive association between GTS and sunlight exposure time, exposure to pesticides, worse health status, and inverse association with wood cultivation. This study presented a high GTS prevalence and suggest that the use of urinary cotinine is a significant biomarker to determine GTS cases, influencing in the distribution by sex. Once the tobacco production involves health hazards, is important to implement measures to prevent the harm caused to tobacco farmers, as set in articles 17 and 18 of the World Health Organization Framework Convention on Tobacco Control.

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Várias mudanças ocorrem no período da adolescência. O adolescente tem que desenvolver habilidades sociais, que são comportamentos que compõem o repertório do indivíduo para responder às demandas sociais adequadamente. Ele também enfrenta situações que podem desencadear estresse, que é a reação do organismo para reestabelecer seu equilíbrio após passar por situação estressora. O objetivo deste estudo foi verificar a relação de habilidades sociais e estresse com as variáveis: sexo, idade, tipo de escola e série. Foram utilizados os testes psicológicos ESA (Escala de Stress para Adolescentes) e IHSA-Del-Prette (Inventário de Habilidades Sociais para Adolescentes). Conforme os resultados encontrados no presente estudo, pode-se concluir que há correlação entre estresse e as variáveis sexo, idade e tipo de escola frequentada, assim como também entre a emissão de habilidades sociais e o tipo de escola frequentada. A hipótese de que as alunas teriam mais habilidades sociais que os alunos não foi confirmada, assim como também não se confirmou que os homens têm mais dificuldades na emissão das respostas do que as mulheres.(AU)

During adolescence many changes occur in adolescent life. They have to develop social skills, behaviors that composes the repertoire of an individual to respond the social demands adequately. They face some situations that can cause stress, which is the reaction of the body to restore its balance after going through stressor situation. The present study aimed to investigate the correlation of social skills and stress variables: sex, age, type of school attended and grade. For this was used Psychological tests ESA (Stress Scale for Adolescents) and IHSA-Del-Prette(Inventory of Social Skills for Adolescents). The obtained results permit to conclude that there is a correlation between stress and the variables gender, age and type of school attended, as well as between the emission of social skills and the type of school attended. The hypothesis that the female students had more social skills than the students was not confirmed, nor it was confirmed that men have more difficulties in issuing the answers than women.(AU)

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We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular ß-glucosidase activity [~ 200 U (g cells)-1 for 200 mM cellobiose and ~ 100 U (g cells)-1 for 2 mM pNPßG] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.7 g L-1 of ethanol. Kinetics of its cellobiase activity demonstrated a high-affinity hydrolytic system inside cells, with Km of 12.4 mM. Our data suggest that, unlike other fungal species that hydrolyze cellobiose extracellularly, both analyzed strains transport it to the cytoplasm, where it is then hydrolyzed by high-affinity intracellular ß-glucosidases. We believe this study increases the fund of knowledge regarding yeasts from Brazilian microbiomes.

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