RESUMEN
As the most abundant biopolymer on earth, cellulose undergoes degradation by a diverse set of enzymes with varying specificities that act in synergism. An assay protocol was developed to detect and quantify activity of cellulose 1,4-ß-cellobiosidase (EC 3.2.1.91) in soil. The optimum pH and temperature for ß-cellobiosidase activity were approximately pH 5.5 and 60 °C, respectively. In the tested six soils, the Michaelis constants (Km) ranged from 0.08 to 0.51 mM, and maximum velocity (Vmax) ranged from 71.5 to 318.1 µmol kg soil-1 h-1. The temperature coefficient (Q10) ranged from 1.72 to 1.99 at non-denaturing temperatures from 10 to 50 °C, and the activation energy (Ea) ranged from 42.5 to 53.7 kJ mol-1. The assay procedure provided reproducible results with a coefficient of variance ≤4.7% and demonstrated a limit of quantification (LOQ) of 50.9 µmol p-nitrophenol release kg-1 soil h-1 for ß-cellobiosidase activity in soil. Notably, the developed assay protocol offers reproducibility and precision comparable to bench-scale assays while reducing costs associated with reagents, supplies, and labor.
Asunto(s)
Celulosa 1,4-beta-Celobiosidasa , Celulosa , Celulosa/metabolismo , Celulosa 1,4-beta-Celobiosidasa/metabolismo , Reproducibilidad de los Resultados , Temperatura , Suelo , CinéticaRESUMEN
Soil salinization is a serious environmental problem that affects agricultural productivity and sustainability worldwide. Organic amendments have been considered a practical approach for reclaiming salt-affected soils. In addition to improving soil physical and chemical properties, organic amendments have been found to promote the build-up of new halotolerant bacterial species and microbial diversity, which plays a critical role in maintaining soil health, carbon dynamics, crop productivity, and ecosystem functioning. Many reported studies have indicated the development of soil microbial diversity in organic amendments amended soil. But they have reported only the development of microbial diversity and their identification. This review article provides a comprehensive summary of the current knowledge on the use of different organic amendments for the reclamation of salt-affected soils, focusing on their effects on soil properties, microbial processes and species, development of soil microbial diversity, and microbial processes to tolerate salinity levels and their strategies to cope with it. It also discusses the factors affecting the microbial species developments, adaptation and survival, and carbon dynamics. This review is based on the concept of whether addition of specific organic amendment can promote specific halotolerant microbe species, and if it is, then which amendment is responsible for each microbial species' development and factors responsible for their survival in saline environments.