RESUMEN
Although certain pollutants can be biologically degraded by microorganisms, rendering their impact short-term, others can not be impaired, such that their effect persists. The present study evaluates the effectiveness of using a field-collected aphid, Aphis gossypii, as a bio-indicator for heavy metals in tomato farms in Riyadh and Hafar Al-Batin, Saudi Arabia. Heavy metals were selected (Cd, Cu, Zn, and Pb) and measured for comparative screening in field-collected plants, soil, and aphids using inductively coupled plasma-mass spectrometry (ICP-MS). Field-collected aphids from both studied regions were identified as Aphis gossypii. In Riyadh, there was no significant difference observed for Cd, Cu, and Zn for all experimental samples, while, Pb was showed differences among samples especially tomato leaves None of the studied samples in Hafar Al-Batin were showed statistically significant differences in Cd, in reverse to significant differences in the other heavy metals. Comparing concentrations of selected heavy metals between the two studied regions was showed that neither region showed a significant difference in heavy metals except for Cu. This study demonstrates that tomato leaf samples showed the highest concentrations of most studied heavy metals, followed by soil, then aphids. Aphids were utilized as a bio-indicator of heavy metals in the studied regions.
RESUMEN
The blowfly Chrysomya albiceps (Diptera: Calliphoridae) has been known to breed in both animal and human carcasses in different geographical regions and is considered an important post-mortem indicator in forensic entomology. Determining the age of its larvae is an essential tool for the accurate determination of post-mortem intervals (PMI). This study adopted a molecular-based approach for age determination of the immature stages of the blowfly, C. albiceps, by evaluating the genes 15_2, 2014192, EcdR, AR, hsp90, and Actin, during larval development at different temperatures. Targeted genes were amplified by real-time PCR (RT-PCR), and the resulting amplicons were normalized against the two housekeeping genes, rp49 and 19_2. Data showed that the expression profile of AR was constant throughout all larval developmental stages at all tested temperatures. 2014192 showed low expression levels throughout the 1st, 2nd, and 3rd larval stages. Hsp90 and EcdR showed different expression profiles. The expression of 15_2 was low at the tested temperatures and was undetectable on most days. Collectively, the results of this study indicate that larvae exhibit temperature-dependent and age-specific up- and down-regulation in the expression profile of the targeted genes. This may indicate a possibility to be utilized as age estimation markers for C. albiceps.
Asunto(s)
Calliphoridae/crecimiento & desarrollo , Perfilación de la Expresión Génica , Larva/crecimiento & desarrollo , Temperatura , Actinas/genética , Animales , Proteínas Portadoras/genética , Entomología Forense , Marcadores Genéticos , Proteínas HSP90 de Choque Térmico/genética , Proteínas de Insectos/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores de Esteroides/genéticaRESUMEN
Aspiculuris tetraptera a pinworm of mice, is an important parasite in institutions with mice colonies for both research and teaching purposes. Infection with this parasite has impact on biomedical research. This is likely due to the availability of the parasite's eggs in the environment, therefore can easily be transmitted and infection is generally asymptomatic. No information regarding the prevalence, morphology or phylogeny is available on A. tetraptera from Saudi Arabia. A group of 50 laboratory mice were investigated for the presence of A. tetraptera. Worms were described morphologically and molecular characterization was attempted using 18S rRNA and Cytochrome Oxidase Subunit I genes. The prevalence of A. tetraptera infestation in the laboratory mice examined was found to be 46%. Morphological description indicated that the worms belong to A. tetraptera and this was confirmed by molecular characterization. Both regions studied have shown that the worm under investigation grouped with A. tetraptera. 18S rDNA sequences obtained in the present study showed high identity with sequences from A. tetraptera while Cytochrome c Oxidase subunit I gene (COI) sequences showed intraspecific variation resulted into two haplotypes from the isolates in the present study. A. tetraptera was recorded for the first time from Saudi Arabia. Molecular characterization has shown, based on the COI sequences, that the Saudi isolates of A. tetraptera are distinct.